CCT α is a novel biomarker for diagnosis of laryngeal squamous cell cancer

Abstract

Choline phosphate-based delivery systems can target the acidic tumor microenvironment. In this study, we set out to evaluate the diagnostic value of Choline phosphate cytidylyltransferase-α (CCTα) in laryngeal squamous cell cancer (LSCC). The expression of CCTα was detected using immunohistochemistry in 50 LSCC patients’ tissues and 16 vocal polyps as control group. Then, clinical data was collected and we used receiver operating characteristic curve (ROC) to estimate the potential of CCTα as diagnostic biomarker. We found CCTα levels to be significantly high in the tissues derived from LSCC patients, ( p < 0.001). Further, we observed a positive correlation of CCTα with tumor size ( p < 0.001), TNM stage ( p < 0.001), lymph node metastasis ( p < 0.001) as well as the grade of LSCC malignancy ( p < 0.001). Furthermore, AUC was determined to be 0.939 by ROC, and the optimal cutoff value 3.100, with 76.0% sensitivity and 100% specificity. We also found an epigenetic basis of CCTα over-expression in LSCC tissues with significantly reduced methylation of CCTα in LSCC tissues, compared to vocal polyps ( p < 0.001). These results support epigenetically-induced over-expression of CCTα as a potential diagnostic marker for LSCC.

Upregulation of CCT-3 Induces Breast Cancer Cell Proliferation Through miR-223 Competition and Wnt/β-Catenin Signaling Pathway Activation

“…In hepatocellular carcinoma, CCT-3, and CCT-8 have been proven as oncogenes that promote cancer cell proliferation (6,7). Notably, CCT-1 and CCT-2 are essential for the survival of breast cancer cells that make them susceptive oncogenes (8)(9)(10)(11). However, the clinical relevance and the function of CCT-3 in breast cancer remain largely unclear.…”

Emergence of Lipid Droplets in the Mechanisms of Carcinogenesis and Therapeutic Responses

“…Indeed, the overexpression of CKα, induced by certain oncogenic signaling pathways such as phosphatidylinositol 3-kinase (PI3K)/Ak strain transforming (Akt) [ 30 ], has been proven in many types of cancers, including CRC. In carcinoma of the larynx, overexpression of CCTα was positively correlated with the size and aggressiveness of the tumors, thus reducing the life expectancy of patients [ 31 ]. The same is true for the enzyme LPCAT1 in hepatocellular carcinoma [ 32 ] and breast cancer [ 33 ].…”

Chaperonin-containing TCP-1 subunit genes are potential prognostic biomarkers and are correlated with Th2 cell infiltration in lung adenocarcinoma: An observational study

“…[11] The biological functions of CCTs and their interacted proteins suggest their potential involvement in various human diseases, including cancer. CCTs have been reported to function as oncogenes in several human cancers, including breast cancer, [12][13][14] laryngeal squamous cell cancer, [15] esophageal squamous cell carcinoma, [16] metastatic LUAD, [17] thyroid neoplasia, [18] and hepatocellular carcinoma. [19][20][21] However, there is a lack of systematic, thorough analysis and understanding of CCTs in LUAD.…”