Cd exposure-triggered metabolic disruption increases the susceptibility of Lymantria dispar (Lepidoptera: Erebidae) larvae to Mamestra brassicae nuclear polyhedrosis virus: A multi-omics study

Jiang, Dun; Tan, Mingtao; Zheng, Lin; Wu, Hongfei; Li, Yaning; Yan, Shanchun

doi:10.1016/j.ecoenv.2022.113280

Cited by 14 publications

(3 citation statements)

References 36 publications

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“…An early microarray study shows that genes related to retinol metabolism in Bombyx mori are stimulated by the infection of cytoplasmic polyhedrosis virus (BmCPV) ( 64 ). Recently, by RNA-seq, retinol metabolism pathway has been revealed responding to the infections of Zika virus in mosquito ( 65 ), and Nuclear Polyhedrosis Virus in moth ( 66 ). It implies that retinol metabolism probably plays a common antiviral role in insects.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Densovirus in Aphid, and Uncovering the Possible Antiviral Process During Its Infection

et al. 2022

Front. Immunol.

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Densoviruses (DVs) are single-stranded DNA viruses and exclusively happen in invertebrates. Most of DVs reported in insects are pathogenic to their native hosts, however, no pathogenic effect of them has been examined in vertebrates. Hence, DVs are the potential agents used in pest managements. Aphids are the primary vectors of plant viruses. In this study, we identified a novel DV in Chinese Sitobion miscanthi population, provisionally named “Sitobion miscanthi densovirus” (SmDV). Taxonomically, SmDV belongs to genus Hemiambidensovirus. In S. miscanthi, SmDV is hosted in diverse cells and can be horizontally transmitted via wheat feeding. Subject to SmDV, aphids activate their intrinsic antiviral autophagy pathway. Grouped with ascorbate and aldarate metabolism, chlorophyll metabolism, p450 related drug metabolism, and retinoid metabolism, aphids form a complex immune network response to the infection of SmDV. Obviously, it works as elder aphids still alive even they contain the highest examined concentration of SmDV. This study provides a foundation for the identifications of novel DVs, and further improves the understanding of the molecular interactions between insects and DVs.

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Section: Discussionmentioning

confidence: 99%

Identification of a Novel Densovirus in Aphid, and Uncovering the Possible Antiviral Process During Its Infection

et al. 2022

Front. Immunol.

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“…Refer to the concentration selection method of Jiang et al [28,29]. The Cd concentrations were 0 (CK), 3.89 (LD) and 51.69 (HD) mg/kg.…”

Section: Insect Rearing and CD Exposurementioning

confidence: 99%

Cadmium Exposure Impairs Development, Detoxification Mechanisms and Gene Expression of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

Zhao,

Miao,

Sheng

et al. 2024

Agronomy

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Cadmium (Cd) is the most serious heavy metal pollutant in the agricultural soil of China and can transfer and accumulate through the food chain and affect the growth, development and physiological processes of phytophagous insect. Glyphodes pyloalis Walker (G. pyloalis) is one of the most important pests of mulberry, and there are few studies on the adverse effects of heavy metals on insects, especially mulberry pests. To understand the toxicology of Cd exposure on G. pyloalis, we investigated the effects of three different concentrations of Cd (0, 3.89 and 51.69 mg/kg, labeled as control check (CK), low dose (LD) and high dose (HD)) on the development and detoxification mechanism of G. pyloalis and explored the molecular mechanism of Cd on G. pyloalis larvae using RNA-seq technology. Transcriptome analysis showed that compared with the CK, a total of 63 differentially expressed genes (DEGs) were identified in LD exposure, including 24 upregulated and 39 downregulated candidates. In CK versus HD groups, 395 upregulated DEGs and 436 downregulated DEGs were identified and the expression patterns of 12 genes related to detoxification and metabolism were verified using qPCR. These DEGs were relevant to multiple specific peroxisome and drug metabolism-cytochrome P450 pathways. Gene annotation and quantitative real-time PCR revealed that a high concentration of Cd significantly stimulated the expression of metabolic detoxification enzyme genes. The results revealed that Cd exposure changed the pupal weight, adult emergence rate and the activities of AKP, ACP and GST in G. pyloalis and induced notable adverse effects at the molecular level on detoxification and metabolism such as concentration and time differences. In addition, we silenced CYP12A2 by RNA interference. Bioassays showed that after silencing CYP12A2, the survival rate of G. pyloalis under Cd exposure was lower than that of the control group.

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“…Human activities such as mining, smelting, urban waste treatment, and electroplating continuously release heavy metal pollutants into water and soil, damaging the ecological environment [1]. In recent years, the issue of heavy metal pollution has been constantly emerging, and the problem of Zn 2+ pollution has gradually become apparent, arousing people's attention.…”

Section: Introductionmentioning

confidence: 99%

Integration of Physiological, Transcriptomic and Metabolomic Reveals Molecular Mechanism of Paraisaria dubia Response to Zn2+ Stress

Wang

Tong

et al. 2023

JoF

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Utilizing mycoremediation is an important direction for managing heavy metal pollution. Zn2+ pollution has gradually become apparent, but there are few reports about its pollution remediation. Here, the Zn2+ remediation potential of Paraisaria dubia, an anamorph of the entomopathogenic fungus Ophiocordyceps gracilis, was explored. There was 60% Zn2+ removed by Paraisaria dubia mycelia from a Zn2+-contaminated medium. To reveal the Zn2+ tolerance mechanism of Paraisaria dubia, transcriptomic and metabolomic were executed. Results showed that Zn2+ caused a series of stress responses, such as energy metabolism inhibition, oxidative stress, antioxidant defense system disruption, autophagy obstruction, and DNA damage. Moreover, metabolomic analyses showed that the biosynthesis of some metabolites was affected against Zn2+ stress. In order to improve the tolerance to Zn2+ stress, the metabolic mechanism of metal ion transport, extracellular polysaccharides (EPS) synthesis, and microcycle conidiation were activated in P. dubia. Remarkably, the formation of microcycle conidiation may be triggered by reactive oxygen species (ROS) and mitogen-activated protein kinase (MAPK) signaling pathways. This study supplemented the gap of the Zn2+ resistance mechanism of Paraisaria dubia and provided a reference for the application of Paraisaria dubia in the bioremediation of heavy metals pollution.

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Cd exposure-triggered metabolic disruption increases the susceptibility of Lymantria dispar (Lepidoptera: Erebidae) larvae to Mamestra brassicae nuclear polyhedrosis virus: A multi-omics study

Cited by 14 publications

References 36 publications

Identification of a Novel Densovirus in Aphid, and Uncovering the Possible Antiviral Process During Its Infection

Identification of a Novel Densovirus in Aphid, and Uncovering the Possible Antiviral Process During Its Infection

Cadmium Exposure Impairs Development, Detoxification Mechanisms and Gene Expression of Glyphodes pyloalis Walker (Lepidoptera: Pyralidae)

Integration of Physiological, Transcriptomic and Metabolomic Reveals Molecular Mechanism of Paraisaria dubia Response to Zn2+ Stress

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