Cd/Zn exposure interactions on metallothionein response in Eisenia fetida (Annelida, Oligochaeta)

Using of chicken antibodies for metallothionein detection in human blood serum and cadmium‐treated tumour cell lines after dot‐ and electroblotting

“…The detection limit of our method is ten times lower compared with that of Demuynck et al [78], where this method was used for MT determination in earthworms as an environmental marker [78,79]. Goncalves et al utilized DIA with mouse monoclonal antibody MT 1/2 UC1MT for detection of MT in cerebrospinal fluid [80].…”

“…MTs containing heavy metals, especially zinc, form covalently bond aggregates, which can be physiologically important for subcellular retention and/or storage of the protein, but the physiological importance of the aggregation remains still unclear [78,79,83,84]. Demuynck et al determined MT content in earthworms exposed to Cd.…”

Uptake and elimination kinetics of metals in soil invertebrates: A review

“…The results also indicate that different species may regulate their Zn body burden at different levels. For example, among earthworms, E. fetida regulates its Zn body concentrations between 100 and 200 mg g À1 dry body weight (Lock and Janssen, 2001;Demuynck et al, 2007) while internal Zn concentrations for E. andrei ranged between 85 and 496 (Van Gestel et al, 1993;Peijnenburg et al, 1999a;Smith et al, 2010), L. rubellus between 416 and 1871 (Ireland, 1979;Hobbelen et al, 2006), D. veneta at approx. 100 (Marinussen et al, 1997b), A. tuberculata up to 400 (Neuhauser et al, 1995), Drawida willsi between 116 and 125 (Panda et al, 1999), and Aporrectodea caliginosa between 676 and 1958 mg g À1 dry body weight (Hobbelen et al, 2006).…”

“…Zn is efficiently regulated in soil organisms by active increase in elimination rate and/or by decreasing uptake rate Lock and Janssen, 2001;Hobbelen et al, 2006;Demuynck et al, 2007;Veltman et al, 2007). Spurgeon and Hopkin (1999) mentioned that the detoxification of Zn in earthworms occurs through excretion.…”

Subcellular distribution of Cd and Zn and MT mRNA expression in the hepatopancreas of Sinopotamon henanense after single and co-exposure to Cd and Zn

“…Additionally, Cd is of environmental concern attributing to its high toxicity (Li et al, 2010), while Zn as an essential element shares comparable chemical and physical properties with Cd (Brzóska and Moniuszko-Jakoniuk, 2001;Thévenod, 2010). So it is crucial to understand how metal interactions influence the aquatic organisms (Demuynck et al, 2007). Studies determining the bioaccumulation of metals in aquatic animals have often been focused on the tissue level, and studies on metal interactions are no exception (Devineau and Amiard-Triquet, 1985;Rainbow et al, 2000;Goto and Wallace, 2007).…”