CD133 positive progenitor endothelial cell lines from human cord blood

Paprocka, Maria; Krawczenko, Agnieszka; Duś, Danuta; Kantor, Aneta; Carreau, Aude; Grillon, Catherine; Kiéda, Claudine

doi:10.1002/cyto.a.21092

Cited by 46 publications

(53 citation statements)

References 27 publications

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“…For example, CD271+ cells in the MSCs isolated from deciduous dental pulp are unable to differentiate into osteogenic, chondrogenic and adipogenic lineages, but the fraction of CD271-cells can [Mikami et al, 2011]. In umbilical cord blood, endothelial progenitor cells also express CD271 [Paprocka et al, 2011] and that could be the reason of the limited differentiation exhibited by CB-CD271 cells in this study.…”

Section: Discussionmentioning

confidence: 75%

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Watson

Foo

et al. 2013

Cells Tissues Organs

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CD271 has been applied to isolate mesenchymal stem cells (MSCs) from bone marrow and other tissues. Umbilical cord blood is a unique resource of stem cells and endothelial progenitor cells. Isolation of MSCs from umbilical cord blood, however, has been inefficient and inconsistent. This study was designed to examine the potential application of CD271 as a marker for the isolation of MSCs from umbilical cord blood. CD271+ cells were isolated from umbilical cord blood and bone marrow using CD271 antibody-conjugated microbeads, and characterized in osteogenic, chondrogenic and adipogenic differentiation. CD271+ cells from umbilical cord blood were slow to proliferate compared with those isolated from bone marrow. While CD271+ cells from bone marrow differentiated into osteogenic, chondrogenic and adipogenic lineages, there were no sound indications of differentiation by CD271+ cells from umbilical cord blood under the same differentiation conditions applied to the CD271+ cells from bone marrow. The study also found that bone marrow CD271+ cells remarkably upregulated the expression of chondrogenic genes under chondrogenic differentiation induction. When implanted into bone defects in mice, CD271+ cells from bone marrow regenerated significant bone, but the counterparts in umbilical cord blood formed little bone in the bone defects. In conclusion, CD271 is an efficient marker for MSC isolation from bone marrow but has failed to isolate MSCs from umbilical cord blood. CD271+ cells in bone marrow are particularly chondrogenic. The property of CD271+ cells is unique but varies from different tissues.

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Section: Discussionmentioning

confidence: 75%

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Watson

Foo

et al. 2013

Cells Tissues Organs

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“…However, we did not test this feature in the present study. Similarly to the herein examined TAg cells, SV40 T-antigen immortalized endothelial progenitor cells (EPCs) from human cord blood were shown to be strongly positive for CD146 [28], as well as hTERT immortalized EPCs [29]. A recent study describes CD146 expression in mouse p19 ARF-/- , which show longer tumor latency as p53 -/- mice [30].…”

Section: Resultsmentioning

confidence: 99%

Comparative Study of MSCA-1 and CD146 Isolated Periosteal Cell Subpopulations

Umrath

Thomalla

Pöschel

et al. 2018

Cell Physiol Biochem

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Background/Aims: Periosteal tissue is a valuable source of multipotent stem cells for bone tissue engineering. To characterize these cells in detail, we generated an immortalized human cranial periosteal cell line and observed an increased MSCA-1 and CD146 expression, as well as an earlier and stronger mineralization compared to the parental cells. Further, we detected a higher osteogenic potential of MSCA-1^high compared to MSCA-1^low cranial periosteal cell (CPC) fractions. In the present study, a possible synergism of MSCA-1 and CD146 for periosteal cell mineralization was investigated. Methods: MSCA-1/CD146 positive and negative CPCs were magnetically isolated (MACS) or sorted by flow cytometry (FACS) and subjected to osteogenic differentiation. The expression of osteogenic marker genes in the four subpopulations was analyzed by quantitative real-time PCR. Furthermore, the co-expression of osteogenic markers/antigens was analyzed by multispectral imaging flow cytometry (ImageStream, AMNIS). The mineralization potential was assessed by the quantification of alizarin stainings. Results: While the total cell yield after separation was higher using MACS compared to the FACS approach, the isolation of MSCA-1^+/- and CD146^+/- subpopulations was more efficient with the FACS separation. The accuracy of the FACS separation of the four distinguished cell subpopulations was confirmed by multispectral imaging flow cytometry. Further, we detected increasing levels of MSCA-1 and CD146 during in vitro differentiation in all subpopulations. However, MSCA-1 expression was significantly higher in the MSCA-1⁺/CD146⁺ and MSCA-1⁺/ CD146^- subpopulations, while CD146 expression remained clearly lower in these fractions. Significantly higher gene expression levels of osteogenic markers, ALP and RUNX2, were detected in MSCA-1⁺ compared to MSCA-1^- CPCs at different time points during in vitro differentiation. Staining and quantification of calcium phosphate precipitates revealed a significantly higher mineralization potential of MACS separated MSCA-1⁺ and CD146^- CPCs, compared to their respective counterparts. FACS sorted CPCs displayed earlier mineralization in both MSCA-1⁺ fractions (d13), while later (d28) only the CD146⁺/MSCA-1^- fraction had a significantly lower calcium phosphate concentration compared to all other fractions. Conclusion: Our results demonstrate, that MSCA-1⁺ cells isolated from CPCs represent a subpopulation with a higher osteogenic potential. In contrast, we found a lower osteogenic potential in CD146⁺ CPCs. In conclusion, only MSCA-1, but not CD146, is a suitable marker for the isolation of osteoprogenitors from CPCs.

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“…Two cell lines, namely HEPC-CB.1 (55.1) and HEPC-CB.2 (55.2), characterised previously by Paprocka and coworkers [18], were isolated from human cord blood. The EP 55.1 cells were cultured in OptiMEM medium (Invitrogen) supplemented with 3 % FBS, 2 mM glutamine and antibiotics (100 U/ml penicillin, 100 lg/ml streptomycin), under a humidified atmosphere of 5 % CO 2 in air at 37°C.…”

Section: Cell Culture Conditionmentioning

confidence: 99%

“…Paprocka and co-workers described two cell lines of human endothelial cells, isolated from the cord blood and successfully immortalised. These cell lines, named initially HEPC-CB.1 and HEPC.CB-2, express a panel of stem and endothelial cell markers but not the markers of mature, differentiated endothelial cells [18]. EPCs are involved in ischaemic regeneration by angiogenesis and myogenesis modulation and remodelling of the ischaemic cardiac tissue [16,19].…”

Section: Introductionmentioning

confidence: 99%

The role of FGF2 in migration and tubulogenesis of endothelial progenitor cells in relation to pro-angiogenic growth factor production

et al. 2015

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In recent years, special attention has been paid to finding new pro-angiogenic factors which could be used in gene therapy of vascular diseases such as critical limb ischaemia (CLI). Angiogenesis, the formation of new blood vessels, is a complex process dependent on different cytokines, matrix proteins, growth factors and other pro- or anti-angiogenic stimuli. Numerous lines of evidence suggest that key mediators of angiogenesis, vascular endothelial growth factor (VEGF) and hepatocyte growth factor (HGF) together with fibroblast growth factor2 (FGF2) are involved in regulation of the normal and pathological process of angiogenesis. However, less information is available on the complex interactions between these and other angiogenic factors. The aim of this study was to characterise the effect of fibroblast growth factor2 on biological properties of human endothelial progenitor cells with respect to the expression level of other regulatory cytokines. Ectopic expression of FGF2 in EP cells stimulates their pro-angiogenic behaviour, leading to increased proliferation, migration and tube formation abilities. Moreover, we show that the expression profile of VEGF and other pro-angiogenic cytokines, such as HGF, MCP2, and interleukins, is affected differently by FGF2 in EPC. In conclusion, we provide evidence that FGF2 directly affects not only the biological properties of EP cells but also the expression pattern and secretion of numerous chemocytokines. Our results suggest that FGF2 could be applied in therapeutic approaches for CLI and other ischaemic diseases of the vascular system in vivo.

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CD133 positive progenitor endothelial cell lines from human cord blood

Cited by 46 publications

References 27 publications

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

CD271 as a Marker for Mesenchymal Stem Cells in Bone Marrow versus Umbilical Cord Blood

Comparative Study of MSCA-1 and CD146 Isolated Periosteal Cell Subpopulations

The role of FGF2 in migration and tubulogenesis of endothelial progenitor cells in relation to pro-angiogenic growth factor production

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