CD166 expression in dentigerous cyst, keratocystic odontogenic tumor and ameloblastoma

Abstract

BackgroundCD166 is a glycoprotein of an immunoglobulin super family of adhesion molecules that has been associated with aggressive characteristics and high recurrence rate of tumors. Different odontogenic lesions exhibit considerable histological variation and different clinical behavior. In an attempt to clarify the mechanisms underlying this different behavior, the present study investigates the immunohistochemical expression of CD166 in these lesions.Material and MethodsIn this study 69 formalin-fixed, paraffin embedded tissue blocks of odontogenic lesion consist of 15 unicystic ameloblastoma (UA), 17 solid ameloblastoma (SA), 18 keratocystic odontogenic tumors (KCOT), and 19 dentigerous cysts (DC) were reviewed by immunohistochemistry for CD166 staining.ResultsIn this study, CD166 immune staining was evident in all specimen groups except dentigerous cyst. In positive cases, protein localization was cytoplasmic and/or membranous. CD166 expression was seen in76.5% (13) of SA, 73.5% (11) of UA, and 66.7% (12) of KCOTs. Statistical analysis showed that CD166 expression levels were significantly higher in ameloblastoma (SA and UA) and KCOTs than dentigerous cyst (P <0.001), but there was no statistically significant difference between CD166 expression in the other groups (P>0.05).ConclusionsThis data demonstrates that overexpression of CD166 may have a role in the pathogenesis of ameloblastoma and KCOT. Key words:CD166, ameloblastoma, dentigerous cyst, odontogenic keratocyst.

Immunohistochemical Expression of Cortactin, E-Cadherin, and MDM2 Proteins in Solid Ameloblastoma versus Odontogenic Keratocyst (An immunohistochemical study)

“…In the current study, the mean value of area percent of E-cadherin immunostaining was higher in OKC than SAB with no statistically significant difference between them. These results are in line with other's results (42) confirmed that adhesion molecules as (CD166) were expressed in ameloblastoma and OKC with no statistically significant difference between them. This could be explained by decreased to the negative reaction of E-cadherin in peripheral basal cells of both lesions where they express EMT phenotype and have the potential of focal invasion.…”

The expression of BMI-1 in ameloblastoma and dentigerous cyst

“…16,20 This reflects the low activity in the proliferative component of the lining and consequently the known indolent course of DC. 3,5,16,21,22 An original finding in our research is the dual staining pattern, nuclear and cytoplasmic, with a predominance of the former pattern in the Am group of neoplastic nature. The cytoplasmic immunoreaction in the current study is justified by the study which proved the presence of BMI-1 in the inner mitochondrial membrane.…”

“…2,3 A dentigerous cyst (DC) is the most common type of developmental odontogenic cyst with an indolent behavior and low recurrence rate. 4,5 These cysts are usually asymptomatic and are detected by routine radiographic examination except in large cases. 6 Larger cysts make great resorption of bone leading to thinning of cortex and eggshell cracking on palpation.…”

The Stem Cell Expression Profile of Odontogenic Tumors and Cysts: A Systematic Review and Meta-Analysis

“…AMBL was found to express ABCG2 [ 13 ], ALDH1 [ 34 ], BMI1 [ 13 , 40 ], CD133 [ 13 ], CD166 [ 41 ], CD44 [ 12 , 31 , 44 ], and c-Myc [ 45 ] in peripheral and/or central cells of the parenchyma at variable extent and intensity levels, as well as CD34 in spindle-shaped stomal cells [ 10 ] and LGR5 [ 39 ] and NANOG [ 10 , 14 ] in both epithelial and stromal tumor components. AMBL cases were completely negative for OCT4 in four studies [ 10 , 12 , 28 , 32 ], and 100% positive in epithelial cells in two studies [ 14 , 31 ].…”

“…In contrast, five studies revealed SOX2 positivity in the majority of peripheral and/or central cells of epithelial islands [ 6 , 14 , 33 , 38 ] and/or stomal cells [ 6 , 10 ]. In most studies reporting stem cell marker expression data in different histopathological variants of AMBL, no significant differences were observed between the microscopic variants [ 6 , 13 , 34 , 41 , 42 , 45 ]. Unicystic AMBL expressed CD166 in the cystic lining [ 41 ], CD34 and NANOG in stromal cells [ 10 ], and SOX2 in epithelial [ 38 ] and stromal cells [ 10 ], while it was OCT4 negative [ 10 ].…”