CD1a- and CD83-positive dendritic cells as prognostic markers of metastasis development in early breast cancer patients

Giorello, María Belén; Matas, Ayelén; Marenco, Pablo; Davies, Kevin; Borzone, Francisco Raúl; Calcagno, María de Luján; García‐Rivello, Hernán; Wernicke, Alejandra; Martinez, Leandro Marcelo; Labovsky, Vivian; Chasseing, Norma Alejandra

doi:10.1007/s12282-021-01270-9

Cited by 26 publications

(19 citation statements)

References 45 publications

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“…IHC staining was performed using CD83 antibody, a marker of mature DCs (15,16). CD83 expression was significantly higher in RCC than in LCC (Figure 4B).…”

Section: Analysis Of Differences In Immune Infiltration In Rcc and Lccmentioning

confidence: 99%

Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer

Ding²,

Wu³

et al. 2022

Front. Oncol.

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BackgroundGrowing evidence suggests that colorectal cancer (CRC) should be considered a heterogeneous disease. The right side (RCC) and left side (LCC) colorectal cancer have different clinical characteristics and immune landscapes. The aim of this study was to analyze differential expression and prognostic correlation of immune-related factors between RCC and LCC.MethodsThe gene expression profile and clinical characteristics of CRC patients were retrieved from The Cancer Genome Atlas data portal (n=525). Using a deconvolution algorithm, immune cell infiltration in RCC and LCC based on the RNA-seq data was analyzed. Differentially expressed genes (DEGs) were obtained by performing differential gene expression analysis. Immune-related DEGs were derived by the intersection with immune-related factors downloaded from the IMMPORT database. To further validate the findings, we applied immunohistochemical (IHC) staining of a CRC tissue microarray (TMA). The distribution of immune cells in RCC and LCC and changes in the expression of immune molecules on their membranes were verified. The expression levels of circulating cytokines were measured by flow cytometry to detect the cytokines secreted by immune cells in RCC and LCC. Furthermore, to reveal the prognostic value of differential immune factors on RCC and LCC patients, survival analysis based on mRNA levels using TCGA cohort and survival analysis using protein levels was performed using our CRC patients.ResultsThe infiltration of immune cells differed between RCC and LCC, the infiltration degree of macrophages M0 was significantly higher in LCC, while the infiltration degree of differentiated macrophages M1 and M2, CD4+ T and CD8+ T cells was significantly higher in RCC. The expression of related molecules by immune cells also differed between RCC and LCC. The expression of 7 genes in RCC was higher than that in LCC, which were CCR5, CD209, CD8A, HCK, HLA-DPB1, HLA-DQA1, HLA-DRA, respectively. Meanwhile, the expression of 2 genes in LCC was higher than in RCC, which were IL-34 and PROCR. Patients with RCC having high expression of HLA-DQA1 mRNA or proteins had better survival and LCC patients with high expression of IL 34 mRNA or protein had better survival.ConclusionsIn this study, we comprehensively compared differences in immune cells and regulating factors between left and right colorectal cancer. Different expression patterns and their effects on survival were identified. The analysis of immune-related factors may provide a theoretical basis for precise immunotherapy of RCC and LCC.

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“…IHC staining was performed using CD83 antibody, a marker of mature DCs (15,16). CD83 expression was significantly higher in RCC than in LCC (Figure 4B).…”

Section: Analysis Of Differences In Immune Infiltration In Rcc and Lccmentioning

confidence: 99%

Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer

Ding²,

Wu³

et al. 2022

Front. Oncol.

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“…DC-LAMP (CD208), another marker of mature DCs and a lysosome-associated membrane glycoprotein, exhibits greater specificity for DCs [14]. CD1a and CD1c represent transmembrane glycoproteins that are homologous with MHC molecules but are involved in the presentation of nonpeptide antigens (lipids and glycolipids) [15,16]. Both are regarded as immature DC markers.…”

Section: Discussionmentioning

confidence: 99%

“…However, the TME considerably impacts dendritic cell functionality. It contains many immunosuppressive factors such as VEGF, IL-6, IL-10, PGE2, and LXRalfa that are delivered from cancerous and other residual cells [1,15] that limit DCs' migratory capacity and inhibit their maturation as well as their efficient antigen presentation [17,30]. Neoplasms trap dendritic cells by hindering their capabilities to travel to LNs and promoting apoptosis through FasL and TRAIL [30].…”

Section: Discussionmentioning

confidence: 99%

Distribution of DC Subtypes: CD83+, DC-LAMP+, CD1a+, CD1c+, CD123+, and DC-SIGN+ in the Tumor Microenvironment of Endometrial Cancers—Correlation with Clinicopathologic Features

Dyduch

Miążek

Laskowicz³

et al. 2023

IJMS

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Treatment options for endometrial cancer (EC) do not provide satisfactory survival improvement for advanced cases, hence the interest in novel therapies utilizing immunological regulatory mechanisms. Measures to modify the functionality of dendritic cells (DCs) found in TME are intensively investigated, given that DCs play a crucial role in inducing antitumor immunity. Samples of malignant endometrial neoplasms obtained from 94 patients were immunohistochemically stained with selected antibodies. Counts of positively identified DCs were correlated with clinical advancement and histological malignancy of cancers. The most prominent DC subtypes were immature DC-SIGN+ or CD123+. Mature CD83+ DCs were the fewest. We found a significant divergence of grade value distribution between cancers of different DCs’ CD1a+ counts. The DC-LAMP+ count was positively associated with grade. Cancers with the least DC CD1c+ or DC CD123+ had higher pT scores than ones that were more heavily infiltrated. ECs can suppress immune cells, hence the predominance of immature DCs in our samples. Associations between DC counts and clinicopathological features of EC were observed only for a few subsets, which was plausibly due to the low diversity of the obtained samples or the small group size. Predictive abilities of particular DC immune subsets within EC’s TME remain ambiguous, which calls for further research.

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“…This process is associated with high expression of costimulatory receptors on DCs. CD83 is usually known as markers for mature DCs [ 28 , 29 ], and costimulatory receptors including CD40, CD80, and CD86 also played important roles in DC-initiated T cell proliferation [ 30 ]. In the present study, we created an IFI44L-overexpression environment through plasmid transfection into normal monocytes and induced them differentiating into Mo-DCs to simulate the changes in the human body.…”

Section: Discussionmentioning

confidence: 99%

Epigenetic Regulation of IFI44L Expression in Monocytes Affects the Functions of Monocyte-Derived Dendritic Cells in Systemic Lupus Erythematosus

Luo

et al. 2022

Journal of Immunology Research

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Background. Interferon-inducible 44 like (IFI44L) is a newly discovered interferon-induced gene and has been reported to overexpress in systemic lupus erythematosus (SLE). However, little is known about the mechanism and function of IFI44L overexpression in SLE. In this study, we aimed to investigate the epigenetic mechanism of IFI44L overexpression in SLE monocyte and its potential functions contributing to the pathogenesis of SLE. Methods. We collected peripheral blood from 20 SLE patients and 20 healthy controls. Expression of IFI44L in monocytes and effects of different signal transducers and activators of transcription (STAT) pathway inhibitors on IFI44L expression were detected. Recruitment of ten-eleven translocation protein (TET) by STAT and methylation of IFI44L promoter were evaluated. Effects of IFI44L overexpression on the expression of surface markers on monocyte-derived dendritic cells (Mo-DCs) were analyzed. T cell differentiation mediated by Mo-DCs and related cytokines production were also analyzed. Results. Expression level of IFI44L was significantly increased in SLE monocyte. IFI44L expression was decreased most significantly in STAT3 inhibitor compared with other inhibitors. STAT3 regulated IFI44L expression and interacted with TET2 which induced DNA demethylation of IFI44L promoter. Overexpression of IFI44L in monocyte enhanced the maturation and functions of Mo-DC by upregulating costimulatory receptors and inducing Th1/Th17-related cytokines when cocultured with naïve CD4+ T cells. Conclusion. TET2 recruited by STAT3 induces DNA demethylation of IFI44L promoter which promotes IFI44L overexpression in monocyte contributing to the pathogenesis of SLE by enhancing the maturation and functions of Mo-DC. IFI44L is expected to become a new target for treatment of SLE.

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CD1a- and CD83-positive dendritic cells as prognostic markers of metastasis development in early breast cancer patients

Cited by 26 publications

References 45 publications

Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer

Differential Expression and Prognostic Correlation of Immune Related Factors Between Right and Left Side Colorectal Cancer

Distribution of DC Subtypes: CD83+, DC-LAMP+, CD1a+, CD1c+, CD123+, and DC-SIGN+ in the Tumor Microenvironment of Endometrial Cancers—Correlation with Clinicopathologic Features

Epigenetic Regulation of IFI44L Expression in Monocytes Affects the Functions of Monocyte-Derived Dendritic Cells in Systemic Lupus Erythematosus

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