CD20 expression: A risk stratification factor for newly diagnosed multiple myeloma with t(11;14)

Jian, Yuan; Zhang, Zhiyao; Zhou, Huixing; Yang, Guangzhong; Geng, Chuanying; Wang, Huijuan; Gao, Wen; Chen, Wen-Ming

doi:10.3389/fonc.2022.1061438

Cited by 4 publications

(3 citation statements)

References 33 publications

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“…26 Although CD20 is a transmembrane phosphoprotein commonly expressed at different stages of B lymphocyte differentiation, its atypical expression in plasmacytic neoplasms is poorly described, and it has already occurred in cases of MM in humans and extramedullary plasmacytomas in dogs. 24,27 The presence of hypercalcemia, Bence-Jones proteinuria, and extensive bone lysis are considered negative prognostic factors in the dog, 5 but none of these alterations were observed in the present case reports. However, the presence of CD20 marking in neoplastic plasma cells from the cell blocks of two animals may be related to longer survival times after diagnosis and initiation of adequate treatment.…”

Section: Discussionmentioning

confidence: 45%

“…However, the presence of CD20 marking in neoplastic plasma cells from the cell blocks of two animals may be related to longer survival times after diagnosis and initiation of adequate treatment. A study of a specific cytogenetic group of humans newly diagnosed with MM proposes that unusual CD20 expression may be a favorable prognostic factor with a tendency toward longer survival, 27 which, despite the small number of cases showed in this study, suggests that the use of cell block and immunohistochemistry may also be relevant in the investigation of a possible prognostic factor in dogs with MM, which should be studied.…”

Section: Discussionmentioning

confidence: 76%

“…The absence of staining for PAX5, CD79a excluded the presence of B lymphocytes and CD3 of T lymphocytes, although expression of MUM1 associated with CD3 in peripheral T‐cell lymphoma or CD20 in diffuse large B‐cell lymphoma has recently been reported in dogs 26 . Although CD20 is a transmembrane phosphoprotein commonly expressed at different stages of B lymphocyte differentiation, its atypical expression in plasmacytic neoplasms is poorly described, and it has already occurred in cases of MM in humans and extramedullary plasmacytomas in dogs 24,27 …”

Section: Discussionmentioning

confidence: 99%

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Multiple myeloma in dogs: Use of the cell block technique as a new diagnostic tool

Valente,

Peleteiro,

Dias

et al. 2024

Veterinary Clinical Pathol

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BackgroundThe diagnosis of multiple myeloma (MM) in dogs may be challenging and complex. The cell blocks are a diagnostic technique that allows the characterization of neoplastic cells and, therefore, might help in the diagnosis of atypical MM.ObjectiveThe objective of the present work is to describe three clinical cases in which the cell blocks and immunohistochemistry contributed to the definitive diagnosis of canine MM.MethodsThree dogs, one female and two males, with different clinical signs, were presented for consultation with anemia, hyperproteinemia with monoclonal gammopathy, and the presence of plasmacytosis in the bone marrow. Cytologic analysis of the spleen was performed in two dogs and was suggestive of the presence of lymphocytes or plasma cells of a neoplastic nature in one of the cases and plasma cell hyperplasia associated with extramedullary hematopoiesis in the other. Given the hypotheses of lymphoid neoplasms with a plasma cell phenotype, cell blocks from aspiration punctures were performed for immunohistochemical analysis with anti‐CD3, CD20, CD79αcy, PAX5, and MUM1 antibodies.ResultsThe results revealed positive staining for MUM1 in 80% of the cells in the spleen cell block and for CD20 and MUM1 in 70% of the cells in the bone marrow cell blocks, with negative staining for the other antibodies. The immunophenotyping results allowed the diagnosis of MM in the three cases and excluded other lymphoid neoplasms.ConclusionsThis work reinforces the importance of using cell blocks in the diagnosis of neoplasms by demonstrating their potential to aid the diagnosis of MM.

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