CD24 is an independent prognostic marker of survival in nonsmall cell lung cancer patients

Abstract

Originally identified as a B-cell marker, expression of the cell surface molecule CD24 has meanwhile been observed in a variety of human malignancies. It appears to function as a ligand of P-Selectin, an adhesion molecule that is present in activated platelets and endothelial cells. We aimed to determine the rate of CD24 expression in our nonsmall cell lung cancer (NSCLC) collection and to clarify its correlation with clinicopathological parameters including patients' survival. A total of 89 NSCLC were analysed immunohistochemically using a monoclonal CD24 antibody (clone 24C02) and a standard detection system (LSAB, DAKO) on NSCLC tissue microarrays (TMA). The staining was semiquantitatively scored (0, 1+, 2+, 3+) and grouped into high (2+, 3+)-and low (0, 1+)-level expression for statistical analysis. A high level of CD24 expression was observed in 45% of the cases, preferentially adenocarcinomas. Patients whose tumours had a high CD24 expression showed a significantly shorter median survival time of 23 months vs 38 months (P ¼ 0.033, log-rank test). Similarly tumour, grading, nodal status and clinical stage were significant prognostic markers in univariate survival analysis. Importantly, in the Cox regression-based multivariate analysis, CD24 expression (P ¼ 0.025) together with tumour stage (P ¼ 0.006) and grade (P ¼ 0.011) proved to be independent prognostic parameters. We hypothesise that the decreased survival of NSCLC patients with strongly CD24-positive tumours is related to an enhanced propensity of haematogenous metastasis formation, which might be P-Selectin mediated.

Repressor roles for TCF-4 and Sfrp1 in Wnt signaling in breast cancer

“…Although CD24 has been reported to be a marker for human breast cancers (Kristiansen et al, 2003) we did not observe any increased expression in human breast tumors that we analysed (data not shown) and we are not purporting a functionally significant role it in neoplastic transformation by Wnts. In this study, we simply used CD24 as an endogenous marker for Wnt signaling in MCF10A cells in order to study the role of TCF-4 as a transcriptional repressor.…”

CD24: from A to Z

“…96,97 In non-small cell lung cancer, CD24 expression is an independent marker for the overall survival of cancer patients. 102 Moreover, in esophageal squamous cell carcinoma, CD24 expression correlates with tumor lymph node metastasis, tumor grade and survival time. 88 Similar observations were found in many types of cancer, including cholangiocarcinoma, 90,91 urothelial carcinoma, 93,94 ovarian cancer 95 and prostate carcinomas.…”

Increased expression of ALCAM/CD166 in pancreatic cancer is an independent prognostic marker for poor survival and early tumour relapse

“…Finally, sections were counterstained with haematoxylin, dehydrated in graded concentrations of ethanol and mounted. The staining intensity of each slide was scored as absent: 0, weak: 1, medium: 2 and strong: 3 as described before (Kristiansen et al, 2003) on a blind basis by three independent researchers (CK, HW, NM) and two pathologists (CM, FB). Besides, for ALCAM, clinical specimen were characterised as membranous-positive or as non-membranous staining.…”