CD3-associated heterodimeric polypeptides on suppressor hybridomas define biologically active inhibitory cells.

Weiner, David B.; Hanna, Nabil; Bluestone, Jeffrey A.; Coligan, John E.; Williams, William V.; Greene, Mark I.

doi:10.1073/pnas.85.16.6077

Cited by 13 publications

(8 citation statements)

References 33 publications

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“…(11). This observation has been confirmed in other suppressor T-cell systems (12,24,25). Recently, a series of Ts hybridomas was prepared by fusion with the TcR a-73-BW1100 thymoma; all Ts, hybridomas from this fusion express Ts donor-derived TcR a/,8 complexes (10).…”

Section: Discussionsupporting

confidence: 72%

“…Subsequent analyses of Ts hybridomas indicated that Ts do express TcR a-chain mRNA derived from the Ts donor (3,4) and that some express the CD3-TcR a/(3 complex on their surface (5)(6)(7)(8)(9)(10)(11). Selection of Ts hybridomas for expression of surface CD3 resulted in an increase in antigen binding (11) and suppressive bioactivity (11,12). Molecular analysis of three 4-hydroxy-3-nitrophenyl acetyl (NP)-specific suppressor effector (Ts3) hybridomas indicated that each expressed a donor-derived TcR a chain and a TcR (3 chain derived from the BW5147 fusion partner (4).…”

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confidence: 99%

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T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

Kuchroo

Byrne

Atsumi

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionsupporting

confidence: 72%

mentioning

confidence: 99%

T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

Kuchroo

Byrne

Atsumi

et al. 1991

Proc. Natl. Acad. Sci. U.S.A.

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“…A possible resolution of the controversy surrounding TCR in regulatory T cells may come from the recent results of Weiner and colleagues (24). They found that a very small population (1-3%) of T3' cells in their cloned suppressorinducer T-cell hybridoma, F12, was responsible for all of the production of antigen-specific factor by this cell.…”

Section: Discussionmentioning

confidence: 99%

Specific inhibition of cell-surface T-cell receptor expression by antisense oligodeoxynucleotides and its effect on the production of an antigen-specific regulatory T-cell factor.

Zheng

Sahai

Kilgannon

et al. 1989

Proc. Natl. Acad. Sci. U.S.A.

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We have used antisense oligodeoxynucleotides corresponding to genes encoding the variable (V) region of the T-cell receptor (TCR) a and j3 chains (V. and Vp) to control TCR expression in T-cell hybridomas. Two hybridomas, A1.1 and B1.1, recognize a synthetic polypeptide antigen designated poly 18 {poly[Glu-Tyr-Lys-(Glu-Tyr-Ala)51} together with I-Ad. We have found that TCR function (production of lymphokines in response to antigen) and T3 expression were removed after protease treatment of the cells and were fully recovered 48 hr later. However, when antisense oligodeoxynucleotides corresponding to the appropriate TCR V genes were present after protease treatment, little or no recovery of TCR function or T3 expression was observed. This effect was specific for the TCR V genes utilized by the T cell: antisense oligodeoxynucleotides corresponding to the TCR V regions of AM.1 had no effect on TCR expression in B1.1 and vice versa. Thus, antisense oligodeoxynucleotides can be used to temporarily block expression of a TCR gene in a T-cell hybridoma. This technique was then applied to a paradoxical phenomenon in Al.1 cells. We had observed previously that Al.1 releases an antigen-specific immunoregulatory activity that shows the same antigenic fine specificity as is displayed by the TCR of AL.L. We now report that antisense oligodeoxynucleotides corresponding to the AM.1 V. gene blocked the production of this soluble antigen-specific activity by the cell. Antisense oligodeoxynucleotides corresponding to All Vp, on the other hand, had no effect on the production of this antigen-specific activity. We discuss these observations in the context of recent findings on the nature of T cell-derived antigen-specific regulatory factors.T lymphocytes specifically recognize foreign antigen together with self major histocompatibility complex (MHC) molecules through the cell-surface T-cell receptor (TCR) complex. This complex is composed of the TCR a and p chains, which are responsible for antigen and MHC specificity (1), and the T3 molecules, which may be responsible for transducing the membrane signal (2). a Chain, p chain, and T3 are only expressed as a complex; in the absence ofany one component, cell-surface expression does not occur (2).A helper T-cell hybridoma (A1.1) has been described (3) that expresses TCR a and 8 molecules specific for a synthetic polypeptide designated poly 18 {poly[Glu-Tyr-Lys-(Glu-TyrAla)5]} and, in the presence of specific antigen and I-Ad, releases lymphokines. This T-cell hybridoma also constitutively produces a poly 18-specific cell-free factor involved in antigen-specific induction of suppression, which we call "suppressor-inducer factor" (4). Analysis has revealed (4) that the factor produced by Al. 1 displayed the same antigenic fine specificity exhibited by the TCR on the Al.1 cell surface.Further, an anti-TCR antiserum was found to bind the antigen-specific factor (5). These results led us to speculate that at least some of the genes encoding the TCR a and 83 chains may be responsibl...

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“…Our efforts to understand suppressor T cells and their factor(s) began several years ago and have continued to some extent in several systems (22)(23)(24)(25). Recent interest in this area once again is obvious.…”

Section: Discussionmentioning

confidence: 99%

Suppressor T cells regulate the nonanergic cell population that remains after peripheral tolerance is induced to the Mls-1 antigen in T cell receptor Vβ8.1 transgenic mice

Maeda

Fujimoto

Greene

2000

Proc. Natl. Acad. Sci. U.S.A.

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CD3-associated heterodimeric polypeptides on suppressor hybridomas define biologically active inhibitory cells.

Cited by 13 publications

References 33 publications

T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

T-cell receptor alpha chain plays a critical role in antigen-specific suppressor cell function.

Specific inhibition of cell-surface T-cell receptor expression by antisense oligodeoxynucleotides and its effect on the production of an antigen-specific regulatory T-cell factor.

Suppressor T cells regulate the nonanergic cell population that remains after peripheral tolerance is induced to the Mls-1 antigen in T cell receptor Vβ8.1 transgenic mice

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