CD301b+ Dermal Dendritic Cells Drive T Helper 2 Cell-Mediated Immunity

Abstract

Summary Unlike other types of T helper (Th) responses, whether the development of Th2 cells requires instruction from particular subset of dendritic cells (DCs) remains unclear. By using an in vivo depletion approach, we have shown that DCs expressing CD301b were required for the generation of Th2 cells after subcutaneous immunization with ovalbumin (OVA) along with papain or alum. CD301b+ DCs are distinct from epidermal or CD207+ dermal DCs (DDCs) and were responsible for transporting antigen injected subcutaenoulsy with Th2-type adjuvants. Transient depletion of CD301b+ DCs resulted in less effective accumulation and decreased expression of CD69 by polyclonal CD4+ T cells in the lymph node. Moreover, despite intact cell division and interferon-γ production, CD301b+ DC depletion led to blunted interleukin-4 production by OVA-specific OT-II transgenic CD4+ T cells and significantly impaired Th2 cell development upon infection with Nippostrongylus brasiliensis. These results reveal CD301b+ DDCs as the key mediatorsof Th2 immunity.

B Cell–Intrinsic MyD88 Signaling Is Essential for IgE Responses in Lungs Exposed to Pollen Allergens

“…However, recent advances revealed that Tfh cells, rather than Th2 cells or other Th subsets, are important for controlling humoral immunity (43). In addition, previous studies reported the differential regulation of Th2 cell development and Tfh cell-controlled IgE production (43,44). In the current study, Tfh cell development was abrogated in Myd88 2/2 mice with ragweed pollen exposure in the lung.…”

Novel insights into the role of immune cells in skin and inducible skin-associated lymphoid tissue (iSALT)

“…As mentioned above, Langerin + dDCs are shown to induce 1 cells during cutaneous Candida albicans infection [44]. On the other hand, in the sensitization phase of CHS, various literatures indicate the importance and redundancy of di erent DC types, including LCs, Langerin + dDCs, and CD11b + cDCs [45,52,55,56]. e hapten dose and the number of DCs seem to be important, as discussed in the chapter of LCs.…”

“…XCR1 + cDCs are the only subset to express high levels of Langerin/CD207 among dDCs, and are o en called as Langerin + dDCs. LCs and CD11b -cDCs are also shown to express CD301b, also known as macrophage galactose C-type lectin type 2 (Mgl2) [45,52]. Other several di erent surface markers are used to distinguish each population (Tab.…”

“…e importance of CD11b + dDC in the generation of 2 immune responses has also been suggested [52,57]. e function and characteristics of DN cDCs remain uninvestigated.…”

CD11b+DCs rediscovered: implications for vaccination

“…Similarly, the intestine-specific DC population co-expressing CD11b and CD103 is involved in priming Th2 responses during Nippostrongylus brasiliensis infection. Alongside, cutaneous CD11b + DCs induce polarization of Th2 cells after subcutaneous immunization with ovalbumin along with papain or alum, as well as in a model of contact hypersensitivity [14][15][16]. In two of these studies [15,16], the macrophage galactose-type C-type lectin 2 (MGL2/CD301b) was used as a surrogate marker for CD11b + DCs.…”