CD34 cells in somatic, regenerative and cancer stem cells: Developmental biology, cell therapy, and omics big data perspective

Abstract

The transmembrane phosphoglycoprotein protein CD34 has conventionally been regarded as a marker for hematopoietic progenitors. Its expression on these cells has been leveraged for cell therapy applications in various hematological disorders. More recently, the expression of CD34 has also been reported on cells of nonhematopoietic origin. The list includes somatic cells such as endothelial cells, fibrocytes and interstitial cells and regenerative stem cells such as corneal keratocytes, muscle satellite cells, and muscle-derived stem cells. Furthermore, its expression on some cancer stem cells (CSCs) has also been reported. Till date, the functional roles of this molecule have been implicated in a multitude of cellular processes including cell adhesion, signal transduction, and maintenance of progenitor phenotype. However, the complete understanding about this molecule including its developmental origins, its embryonic connection, and associated functions is far from complete. Here, we review our present understanding of the structure and putative functions of the CD34 molecule based upon our literature survey. We also probed various biological databases to retrieve data related to the expression and associated molecular functions of CD34. Such information, upon synthesis, is hence likely to provide the suitability of such cells for cell therapy.Moreover, we have also covered the existing cell therapy and speculated cell therapy applications of CD34 + cells isolated from various lineages. We have also attempted here to speculate the role(s) of CD34 on CSCs. Finally, we discuss number of large-scale proteomics and transcriptomics studies that have been performed using CD34 + cells.

Decreased m6A Modification of CD34/CD276(B7-H3) Leads to Immune Escape in Colon Cancer

“…post-transcriptional or post-translational regulatory mechanism (Kraan et al, 2014;Picarda et al, 2016;Kapoor et al, 2020). Our study confirmed that the expression of CD34 was positively correlated with FTO expression, while the expression of CD276 was positively correlated with the expression of ALKBH5.…”

“…The ligand of CD276 and its immunomodulatory effect have not been fully determined ( Stodden et al, 2008 ). Although CD34/CD276 is widely expressed in mRNA in CRC, the expression of the protein is limited in steady-state, which indicates that there is an important post-transcriptional or post-translational regulatory mechanism ( Kraan et al, 2014 ; Picarda et al, 2016 ; Kapoor et al, 2020 ). Our study confirmed that the expression of CD34 was positively correlated with FTO expression, while the expression of CD276 was positively correlated with the expression of ALKBH5.…”

CT based radiomic approach on first line pembrolizumab in lung cancer

“…Ganeshan B. and colleagues, in a previous study on NSCLC 11 , showed how the texture feature MPP has strong correlation with a peculiar characteristic of the tumor detected by the staining with pimonidazole, an exogenous marker of hypoxia. Moreover, MPP also correlates inversely with tumor CD34 expression which is a marker of angiogenesis expressed both on hematopoietic and somatic cells 28 . Similar results were also confirmed by another interesting study performed by Hayano K. and colleagues where Authors assessed the performance of predicting survival with TA in patients with NSCLC treated with antiangiogenetic chemotherapy and assessed with both CT and PET/CT 29 .…”

Immunophenotypic and molecular profile of cancer stem‐cell markers in ex vivo canine transmissible venereal tumour (CTVT)

“…Likewise, CD34 has been detected at the transcript level in several cancers, including but not limited to renal, thyroid, breast and stomach cancer. However, at the protein level, CD34 is detected in fewer cancer types such as colorectal, thyroid and melanoma (Kapoor et al, 2019), and this condition may be similar in animals, which could be another explanation for the findings. CD34 + has been correlated…”