CD4 and CD8 expression by human and mouse thymic dendritic cells

Winkel, Kenneth D.; Sotzik, Frank; Vremec, David; Cameron, Paul; Shortman, Ken

doi:10.1016/0165-2478(94)90178-3

Cited by 53 publications

(41 citation statements)

References 24 publications

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“…However, the expression of CD8␣ by the lymphoid-related DC does not appear to be essential for either their development or their function (18,20), so it may be a relic of their particular developmental history. No CD8 expression has been detected on human DC (21).…”

Section: Endritic Cells (Dc) 3 Collect Transport and Then Presentmentioning

confidence: 99%

“…In what at that time appeared as a striking contrast, a proportion of human and rat DC stained strongly for surface CD4 (21,23,24). However, Salomon et al (13) have since reported CD4 staining of a subgroup of mouse lymph node DC, and Pulendran et al (25) have noted the occurrence of CD4 staining on a subgroup of Flt3 ligand-treated mouse spleen DC.…”

Section: Endritic Cells (Dc) 3 Collect Transport and Then Presentmentioning

confidence: 99%

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CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

Vremec

Pooley

Hochrein

et al. 2000

The Journal of Immunology

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719

691

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The dendritic cells (DC) of mouse spleen and thymus were examined for expression of CD4 and CD8. Provided care was taken to avoid selective extraction or selective depletion of DC subpopulations, three main types of DC were detected in mouse spleen: a major new population of CD4+8− DEC-205low CD11bhigh DC, together with the previously described CD4−8− DEC-205low CD11bhigh DC and CD4−8αα+ DEC-205high CD11blow DC. The CD4 on the surface of the CD4+ splenic DC subpopulation was produced by the DC themselves, and CD4 RNA transcripts were present. Likewise, the CD8α on the surface of the splenic CD8+ DC was shown to be a product of the DC themselves, in agreement with earlier evidence. All three spleen DC types would be considered as mature, based on expression of CD80, CD86, and CD40 as well as on T cell stimulating function. Mouse thymuses appeared to contain two DC types; both were DEC-205highCD11blow, but they differed in the level of CD8αα expression. However, as well as this authenticated marker expression, immunofluorescent staining was also found to reflect a series of artifacts, due to the autofluorescence of contaminating cells and due to pickup of CD4 and CD8αβ. By constructing mice chimeric for the hemopoietic lineages using mixtures of wild-type bone marrow with CD4null or CD8αnull bone marrow, a marked pickup by thymic DC of Ags derived from thymocytes was demonstrated.

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Section: Endritic Cells (Dc) 3 Collect Transport and Then Presentmentioning

confidence: 99%

Section: Endritic Cells (Dc) 3 Collect Transport and Then Presentmentioning

confidence: 99%

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

Vremec

Pooley

Hochrein

et al. 2000

The Journal of Immunology

Self Cite

719

691

View full text Add to dashboard Cite

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“…For isolation and analysis of DCs in murine tissue, organs were disrupted by enzymatic digestion, as described previously (20,21). Briefly, lymph nodes and tumors were finely minced and digested for 20 min at room temperature in RPMI 1640 containing 2% FCS, HEPES, 1 mg/ml collagenase type II (Worthington Biochemical), and 1 g/ml grade II bovine pancreatic DNase I (Boehringer Mannheim).…”

Section: Collagenase-dnase Digestion For DC Analysismentioning

confidence: 99%

Dual Control of Antitumor CD8 T Cells through the Programmed Death-1/Programmed Death-Ligand 1 Pathway and Immunosuppressive CD4 T Cells: Regulation and Counterregulation

Currie

Prosser

McDonnell

et al. 2009

The Journal of Immunology

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Tumors have evolved multiple mechanisms to evade immune destruction. One of these is expression of T cell inhibitory ligands such as programmed death-ligand 1 (PD-L1; B7-H1). In this study, we show that PD-L1 is highly expressed on mesothelioma tumor cells and within the tumor stroma. However, PD-L1 blockade only marginally affected tumor growth and was associated with the emergence of activated programmed death-1+ ICOS+ CD4 T cells in tumor-draining lymph nodes, whereas few activated CD8 T cells were present. Full activation of antitumor CD8 T cells, characterized as programmed death-1+ ICOS+ Ki-67+ and displaying CTL activity, was only observed when CD4 T cells were depleted, suggesting that a population of suppressive CD4 T cells exists. ICOS+ foxp3+ regulatory T cells were found to be regulated through PD-L1, identifying one potentially suppressive CD4 T cell population. Thus, PD-L1 blockade activates antitumor CD8 T cell most potently in the absence of CD4 T cells. These findings have implications for the development of PD-L1-based therapies.

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“…Recently, targeting antigens to DCs by conjugating the antigen to an antibody specific for a certain subset of DCs is suggested as a promising therapy for patients with cancer and for boosting or dampening immune responses. Although the unique ability of CD8α + DCs to cross-present antigens to CD8 + cytotoxic T cells has drawn considerable interest, the relatively poor availability of human lymphoid tissues, the lack of CD8α expression by human DCs (Winkel et al, 1994), and the lack of distinct surface markers to define human DC subsets has hampered direct application of murine results to humans. Under steady state conditions, human DCs in blood can be classified into three groups, based on their reaction with monoclonal antibodies generated against blood DCs: BDCA1 + (blood dendritic cell antigen 1, CD1c) DCs, BDCA2 (LILRA4) +…”

Section: The Human Equivalents Of Cd8αmentioning

confidence: 99%

“…The first distinction of DC subset was based on the expression of a surface marker, CD8α Ardavin et al, 1993;Crowley et al, 1989;Maraskovsky et al, 1996;Nussenzweig et al, 1981;Vremec et al, 1992;Winkel et al, 1994;Wu et al, 1996). Unlike T cells, which express heterodimers of CD8α and β chains, DCs express CD8α homodimers.…”

Section: Introductionmentioning

confidence: 99%

The Bloodline of CD8α+ Dendritic Cells

Kang

2012

Molecules and Cells

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CD4 and CD8 expression by human and mouse thymic dendritic cells

Cited by 53 publications

References 24 publications

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

CD4 and CD8 Expression by Dendritic Cell Subtypes in Mouse Thymus and Spleen

Dual Control of Antitumor CD8 T Cells through the Programmed Death-1/Programmed Death-Ligand 1 Pathway and Immunosuppressive CD4 T Cells: Regulation and Counterregulation

The Bloodline of CD8α+ Dendritic Cells

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