CD4 and CD8 molecules can physically associate with the same T-cell receptor.

Gallagher, Pauline; Groth, Barbara Fazekas de St; Miller, J. F. A. P.

doi:10.1073/pnas.86.24.10044

Cited by 40 publications

(21 citation statements)

References 41 publications

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“…Several reports indicate that a minor population of CD8 molecules on resting T cells interacts with the TCR or members of the CD3 complex, as evidenced by immunoprecipitation (9,10). Although we show that interactions between TCR and CD8 (Fig.…”

Section: Discussionsupporting

confidence: 47%

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Monomeric Class I Molecules Mediate TCR/CD3ε/CD8 Interaction on the Surface of T Cells

Block

Johnson

Mendez-Fernandez

et al. 2001

The Journal of Immunology

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Both CD8 and the TCR bind to MHC class I molecules during physiologic T cell activation. It has been shown that for optimal T cell activation to occur, CD8 must be able to bind the same class I molecule that is bound by the TCR. However, no direct evidence for the class I-dependent association of CD8 and the TCR has been demonstrated. Using fluorescence resonance energy transfer, we show directly that a single class I molecule causes TCR/CD8 interaction by serving as a docking molecule for both CD8 and the TCR. Furthermore, we show that CD3ε is brought into close proximity with CD8 upon TCR/CD8 association. These interactions are not dependent on the phosphorylation events characteristic of T cell activation. Thus, MHC class I molecules, by binding to both CD8 and the TCR, mediate the reorganization of T cell membrane components to promote cellular activation.

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Section: Discussionsupporting

confidence: 47%

“…Immunoprecipitation studies have revealed that the majority of CD3 ␦-chains and a minority of TCR␤-chains and CD3␥-, ⑀-, and -chains associate with CD4 or CD8 on resting T cells (9,10). However, there are conflicting reports regarding which interactions between the coreceptors and TCR/CD3 are affected during T cell activation.…”

mentioning

confidence: 99%

Monomeric Class I Molecules Mediate TCR/CD3ε/CD8 Interaction on the Surface of T Cells

Block

Johnson

Mendez-Fernandez

et al. 2001

The Journal of Immunology

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“…Tumor antigens can be presented on both MHC (major histocompatibility complex) class I and II molecules by the "cross-presentation" mechanism, and it was reported that CD4 C and CD8 C co-receptors could physically associate with the same TCR. 33,34 Therefore, we assume that the most enriched CDR3 sequence in CD8 C killer T cells and CD4 C helper cells in P203 would recognize the same tumor antigen presented by MHC class I or class II, and might enhance the host immune system synergistically.…”

Section: Discussionmentioning

confidence: 99%

Characterization of T cell repertoire of blood, tumor, and ascites in ovarian cancer patients using next generation sequencing

et al. 2015

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Tumor-infiltrating lymphocytes (TILs) play an important role in regulating the host immune response and are one of key factors in defining tumor microenvironment. Some studies have indicated that T cell infiltration in malignant ascites is associated with clinical outcome, but few studies have performed detailed characterization of T cell diversity or clonality in malignant effusions. We have applied a next generation sequencing method to characterize T cell repertoire of a set of primary cancers, ascites, and blood from 12 ovarian cancer patients and also analyzed the T cell subtype populations in malignant fluids from 3 ovarian cancer patients. We observed enrichment of certain T cells in tumors and ascites, but most of the enriched T cell receptor (TCR) sequences in tumors and ascites were not common. Moreover, we analyzed TCR sequences of T cell subtypes (CD4 C , CD8 C , and regulatory T cells) isolated from malignant effusions and also found clonal expansion of certain T cell populations, but the TCR sequences were almost mutually exclusive among the three subgroups. Although functional studies of clonally expanded T cell populations are definitely required, our approach offers a detailed characterization of T cell immune microenvironment in tumors and ascites that might differently affect antitumor immune response.

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“…In addition to the affinity of the TCR-pMHC interaction, binding of the CD4 and CD8 coreceptors to MHC class II or I molecules, respectively (20)(21)(22), can influence the overall avidity of the TCR-pMHC association (11,(22)(23)(24)(25). Moreover, the CD4 and CD8 coreceptors are important for signal initiation because they associate to the tyrosine kinase Lck (26), which is required for critical early events in TCR signaling (27).…”

Section: +mentioning

confidence: 99%

Ca2+ Release from the Endoplasmic Reticulum of NY-ESO-1–Specific T Cells Is Modulated by the Affinity of TCR and by the Use of the CD8 Coreceptor

Chen

Morgan²,

Stewart-Jones

et al. 2010

The Journal of Immunology

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CD4 and CD8 molecules can physically associate with the same T-cell receptor.

Abstract: The expression of the cell-surface glycoproteins CD4 and CD8 on functionally mature T cells is usually mutually exclusive and correlates with class II and class I major histocompatibility complex (MHC) restriction, respectively.

Cited by 40 publications

References 41 publications

Monomeric Class I Molecules Mediate TCR/CD3ε/CD8 Interaction on the Surface of T Cells

Monomeric Class I Molecules Mediate TCR/CD3ε/CD8 Interaction on the Surface of T Cells

Characterization of T cell repertoire of blood, tumor, and ascites in ovarian cancer patients using next generation sequencing

Ca2+ Release from the Endoplasmic Reticulum of NY-ESO-1–Specific T Cells Is Modulated by the Affinity of TCR and by the Use of the CD8 Coreceptor

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