CD44 Is Involved in Mineralization of Dental Pulp Cells

“…In our previous study, we identified MSCs in dental pulps using immunohistochemistry (IHC) of CD44 and other putative markers. We found that CD44 was strongly expressed in odontoblasts . A clear expression was also observed on the presecretory ameloblasts, osteoblast, PDL fibroblasts and cementocytes in other studies .…”

supporting

confidence: 83%

Importance of CD44 in the proliferation and mineralization of periodontal ligament cells

Yeh

Yang

Yuan

2014

J of Periodontal Research

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“…Positive selection with CD44, transmembrane glycoprotein and marker of mesenchymal stemness, has permitted us to obtain and isolate putative stem cells that were highly enriched for this particular subset, which were used in our experiments (17,18). Moreover, we have purposely chosen CD44 membrane marker for selecting PDLSCs because of this protein is largely known to be crucial for the proliferation and mineralization of PDL cells and may play multiple important roles in the biological function of periodontal regeneration (19,20). Afterwards, we have established a CD44+ PDL clonal cell line, since this has permitted us to analyze a more homogeneous population and obtain experimental reproducibility, with the final aim to clarify the PDL-regenerative mechanism (21,22).…”

Section: Discussionmentioning

confidence: 99%

The effect of strontium chloride on human periodontal ligament stem cells

Romagnoli

Zonefrati

Galli

et al. 2017

ccmbm

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SummaryThe complete repair of periodontal structures remains an exciting challenge that prompts researchers to develop new treatments to restore the periodontium. Recent research has suggested strontium ion to be an attractive candidate to improve osteogenic activity. In this study, we have isolated a clonal finite cell line derived from human periodontal ligament (PDL) in order to assess whether and in which way different doses of SrCl2 (from 0.5 to 500 μg/ml) can influence both the proliferation and the mineralization process, for future application in oral diseases. PDL cells were cloned by dilution plating technique and characterized by FACS. Cell proliferation analysis and mineralization were performed by [ 3 H]-thymidine incorporation and spectrofluorometric assay. Results have evidenced that the higher SrCl2 concentrations tested, from 25 to 500 μg/ml, have increased the proliferation activity after only 24 h of treatment. Interestingly, the same higher concentrations have decreased the mineralization, which was conversely increased by the lower ones, from 0.5 to 10 μg/ml. Our findings suggest the possible use of SrCl2 in appropriate delivery systems that release, at different time points, the specific dose, depending on the biological response that we want to induce on periodontal ligament stem cells, providing a more efficient periodontal regeneration.

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“…Recently, it has been demonstrated that CD44 is expressed in odontogenic cells with active mineral deposition during tooth development. In case of suppression of CD44 expression in dental pulp cells, their mineralization activities were substantially decreased in both in vitro and in vivo assays [5]. CD117(c-kit)+/CD44+ stromal cells in the adult pulp core could be viewed as undifferentiated perivascular and perineural cells.…”

Section: Discussionmentioning

confidence: 99%