Cdk Activity Couples Epigenetic Centromere Inheritance to Cell Cycle Progression

Silva, Mariana C. C.; Bodor, Dani L.; Stellfox, Madison E.; Martins, Nuno M. C.; Hochegger, Helfrid; Foltz, Daniel R.; Jansen, Lars

doi:10.1016/j.devcel.2011.10.014

Cited by 164 publications

(218 citation statements)

References 52 publications

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“…First, we blocked cells by nocodazole (Supplemental Fig. S7A) to determine whether PTM establishment on new histones requires passage through mitosis, as shown for incorporation of the histone variant CENP-A (Silva et al 2012). We found that H3K9me3 and H3K27me3 acquisition was impeded in nocodazole ( Fig.…”

Section: Cell Cycle Withdrawal Impacts Histone Ptm Levelsmentioning

confidence: 89%

Two distinct modes for propagation of histone PTMs across the cell cycle

et al. 2015

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Epigenetic states defined by chromatin can be maintained through mitotic cell division. However, it remains unknown how histone-based information is transmitted. Here we combine nascent chromatin capture (NCC) and triple-SILAC (stable isotope labeling with amino acids in cell culture) labeling to track histone modifications and histone variants during DNA replication and across the cell cycle. We show that post-translational modifications (PTMs) are transmitted with parental histones to newly replicated DNA. Di-and trimethylation marks are diluted twofold upon DNA replication, as a consequence of new histone deposition. Importantly, within one cell cycle, all PTMs are restored. In general, new histones are modified to mirror the parental histones. However, H3K9 trimethylation (H3K9me3) and H3K27me3 are propagated by continuous modification of parental and new histones because the establishment of these marks extends over several cell generations. Together, our results reveal how histone marks propagate and demonstrate that chromatin states oscillate within the cell cycle.

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Section: Cell Cycle Withdrawal Impacts Histone Ptm Levelsmentioning

confidence: 89%

Two distinct modes for propagation of histone PTMs across the cell cycle

et al. 2015

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“…It is not yet known what chaperone incorporates Cse4 into euchromatin. In most organisms, CENP-A is deposited in G1 when Cdk1 activity is low (Jansen et al 2007;Silva et al 2012), and the Figure 4 Schematic of the yeast centromere. The conserved structure is 120 bp and contains three elements, CDEI, CDEII, and CDEIII.…”

Section: Centromeric Chromatinmentioning

confidence: 99%

The Composition, Functions, and Regulation of the Budding Yeast Kinetochore

2013

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The propagation of all organisms depends on the accurate and orderly segregation of chromosomes in mitosis and meiosis. Budding yeast has long served as an outstanding model organism to identify the components and underlying mechanisms that regulate chromosome segregation. This review focuses on the kinetochore, the macromolecular protein complex that assembles on centromeric chromatin and maintains persistent load-bearing attachments to the dynamic tips of spindle microtubules. The kinetochore also serves as a regulatory hub for the spindle checkpoint, ensuring that cell cycle progression is coupled to the achievement of proper microtubule-kinetochore attachments. Progress in understanding the composition and overall architecture of the kinetochore, as well as its properties in making and regulating microtubule attachments and the spindle checkpoint, is discussed. TABLE OF CONTENTS Abstract 817Introduction 818

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“…Upon anaphase onset, released from the CDKbased inhibition and promoted by the Polo kinase 1 (Plk1)-based stimulation, the Mis18 complex (consisting Mis18a, Mis18b and Mis18 binding protein 1) localizes to the centromere to license the process of new CENP-A recruitment. 7,39 The Mis18 complex is crucial for the recruitment of Holliday junction recognition protein (HJURP), a chaperon that binds to CENP-A in a prenucleosomal complex, to the centromere to execute its nucleosome assembly functions for new CENP-A. 5,6,40 Notably, the localization of HJURP at G1 centromeres appears to be transient, as HeLa cells yield a 2»3 hr time window in early G1 in which HJURP can be detected on the centromere.…”

Section: A New Model Of Epigenetic Regulation Of Centromeric Nucleosomentioning

confidence: 99%

Formin-mediated epigenetic maintenance of centromere identity

Liu

Mao

2016

Small GTPases

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Accurate chromosome segregation in mammalian cells is guided by the centromere, a specialized chromosome region defined by the histone H3 variant centromere protein A (CENP-A). It is not well understood how cells maintain CENP-A levels at centromeres while continuously going through genome replications and cell divisions. A MgcRacGAP-dependent small GTPase molecular switch has been shown as essential for centromeric CENP-A maintenance. By using quantitative imaging, pulse-chase and live cell analysis, a recent work has suggested that the diaphanous formin mDia2, a well-established small GTPase effector, functions downstream of this small GTPase pathway to maintain CENP-A levels at centromeres. A constitutively active mDia2 construct is able to rescue the CENP-A loading defect caused by MgcRacGAP depletion. This study has uncovered an unsuspected role of the cytoskeleton protein mDia2 as an effector of the MgcRacGAP-dependent small GTPase signaling inside the nucleus to participate in the epigenetic regulation of centromere maintenance during cell cycle.

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Cdk Activity Couples Epigenetic Centromere Inheritance to Cell Cycle Progression

Cited by 164 publications

References 52 publications

Two distinct modes for propagation of histone PTMs across the cell cycle

Two distinct modes for propagation of histone PTMs across the cell cycle

The Composition, Functions, and Regulation of the Budding Yeast Kinetochore

Formin-mediated epigenetic maintenance of centromere identity

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