cDNA and genomic DNA sequence of the 21.3 kDa subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa

Nehls, Uwe; Hemmer, Stefanie; Röhlen, D. A.; Pas, Jakub; Preis, Dagmar; Sackmann, U.; Weiss, Hanns

doi:10.1016/0167-4781(91)90074-v

Cited by 16 publications

(10 citation statements)

References 5 publications

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“…Second, exhaustive investigation of the composition of these samples has revealed only a small number of known impurities, in some preparations, which are present at much lower levels than the established subunits of complex I. Third, subunit B14.7 is a homologue of subunit 21.3b from complex I of N. crassa (21).…”

Section: The Subunit Composition Of Complex I From Bovine Heartmentioning

confidence: 99%

“…5). This suggests that subunit B14.7 is part of the membrane arm of complex I, a location reported previously for the N. crassa 21.3b subunit (21). Subunit ESSS-Subunits of subcomplex I␤ were separated by reverse-phase HPLC as shown in Fig.…”

Section: Table I Identification Of Peptides From Subunits B147 and Ementioning

confidence: 99%

“…Thus, the preceding methionine is likely to be the translation initiator. Subunit B14.7 is a homologue of the complex I subunit 21.3b from N. crassa (21); both proteins have similar hydrophobicity profiles with four possible transmembrane spans (see Fig. 5).…”

Section: Table I Identification Of Peptides From Subunits B147 and Ementioning

confidence: 99%

“…Subunit B14.7 has an acetylated N terminus. It is a homologue of subunit 21.3b in complex I from N. crassa (21) and is related to three proteins, Tim17, Tim22, and Tim23, which are involved in protein translocation across the inner mitochondrial membrane (22,23). A mitochondrial import sequence has been cleaved post-translationally from the * This work was supported by the Medical Research Council.…”

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confidence: 99%

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Definition of the Nuclear Encoded Protein Composition of Bovine Heart Mitochondrial Complex I

Carroll

Shannon

Fearnley

et al. 2002

Journal of Biological Chemistry

150

121

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Mitochondrial NADH:ubiquinone oxidoreductase (complex I) from bovine heart is a complicated multisubunit, membrane-bound assembly. Seven subunits are encoded by mitochondrial DNA, and the sequences of 36 nuclear encoded subunits have been described. The subunits of complex I and two subcomplexes (I␣ and I␤) were resolved on one-and two-dimensional gels and by reverse-phase high performance liquid chromatography. Mass spectrometric analysis revealed two previously unknown subunits in complex I, named B14.7 and ESSS, one in each subcomplex. Coding sequences for each protein were identified in data bases and were confirmed by cDNA cloning and sequencing. Subunit B14.7 has an acetylated N terminus, no presequence, and contains four potential transmembrane helices. It is homologous to subunit 21.3b from complex I in Neurospora crassa and is related to Tim17, Tim22, and Tim23, which are involved in protein translocation across the inner membrane. Subunit ESSS has a cleaved mitochondrial import sequence and one potential transmembrane helix. A total of 45 different subunits of bovine complex I have now been characterized.

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Section: The Subunit Composition Of Complex I From Bovine Heartmentioning

confidence: 99%

Section: Table I Identification Of Peptides From Subunits B147 and Ementioning

confidence: 99%

Section: Table I Identification Of Peptides From Subunits B147 and Ementioning

confidence: 99%

mentioning

confidence: 99%

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Definition of the Nuclear Encoded Protein Composition of Bovine Heart Mitochondrial Complex I

Carroll

Shannon

Fearnley

et al. 2002

Journal of Biological Chemistry

150

121

View full text Add to dashboard Cite

show abstract

“…One of these genes (At2g42210) encoding a protein of unknown function was subsequently shown to encode the complex I subunit B14.7 (Meyer et al, 2008;Klodmann et al, 2010;. Complex I from N. crassa, humans, and Chlamydomonas reinhardtii have also been shown to contain a subunit that is similar to the PRAT family proteins, namely, N. crassa 21.3b (GenBank/P25710), human NDUFA11, and C. reinhardtii v2.0/C_180167 (GenBank/AAS58499) (Nehls et al, 1991;Carroll et al, 2002;Murray et al, 2003). In Arabidopsis, this protein has been identified to be a single peripheral subunit of complex I in two independent studies, both of which identified B14.7 in stoichiometric amounts by proteomic analysis (Meyer et al, 2008;Klodmann et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Dual Location of the Mitochondrial Preprotein Transporters B14.7 and Tim23-2 in Complex I and the TIM17:23 Complex in Arabidopsis Links Mitochondrial Activity and Biogenesis

et al. 2012

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Three-dimensional structure of NADH-dehydrogenase from Neurospora crassa by electron microscopy and conical tilt reconstruction

Guénebaut

Vincentelli

Mills

et al. 1997

Journal of Molecular Biology

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143

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cDNA and genomic DNA sequence of the 21.3 kDa subunit of NADH: ubiquinone reductase (complex I) from Neurospora crassa

Cited by 16 publications

References 5 publications

Definition of the Nuclear Encoded Protein Composition of Bovine Heart Mitochondrial Complex I

Definition of the Nuclear Encoded Protein Composition of Bovine Heart Mitochondrial Complex I

Dual Location of the Mitochondrial Preprotein Transporters B14.7 and Tim23-2 in Complex I and the TIM17:23 Complex in Arabidopsis Links Mitochondrial Activity and Biogenesis

Three-dimensional structure of NADH-dehydrogenase from Neurospora crassa by electron microscopy and conical tilt reconstruction

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