cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis

Wang, Chunyan; Zhao, Jianmin; Mu, Changkao; Wang, Qing; Wu, Huifeng

doi:10.1016/j.fsi.2012.11.020

Cited by 33 publications

(10 citation statements)

References 23 publications

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“…To date, only a limited number of GST s have been characterized in mussels. GST pi and omega were identified in P. viridis [ 19 ], while alpha, pi and sigma classes have been identified in M. galloprovincialis [ 45 , 46 ], and pi class GST was found in M. edulis [ 47 ]. Characterization of GSTs has been conducted for other marine bivalves, such as oysters ( C. gigas [ 48 ] and C. ariakensis [ 49 ]), and clams ( R. philippinarum [ 50 – 52 ], Laternula elliptica [ 53 , 54 ], Mercenaria mercenaria [ 55 ], Solen grandis [ 56 ] and A. farreri [ 57 ]).…”

Section: Resultsmentioning

confidence: 99%

“…A recent study on P. viridis showed that GST pi and omega classes showed higher transcriptional levels in the hepatopancreas than in the gonad, gills and mantle [ 19 ]. It was also reported that GST pi and GST sigma 1 in M. galloprovincialis were more highly expressed in the hepatopancreas than in the gills; GSTs alpha, sigma 2 and sigma 3 were found to be more abundant in hemocytes than in the hepatopancreas, gills, mantle, gonad and muscle [ 45 , 46 ]. We also noticed strong tissue-specific expression patterns for the GST genes of P. viridis (Table 5 ).…”

Section: Resultsmentioning

confidence: 99%

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De novo transcriptome analysis of Perna viridis highlights tissue-specific patterns for environmental studies

Leung

Mak

et al. 2014

BMC Genomics

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BackgroundThe tropical green-lipped mussel Perna viridis is a common biomonitor throughout the Indo-Pacific region that is used for environmental monitoring and ecotoxicological investigations. However, there is limited molecular data available regarding this species. We sought to establish a global transcriptome database from the tissues of adductor muscle, gills and the hepatopancreas of P. viridis in an effort to advance our understanding of the molecular aspects involved during specific toxicity responses in this sentinel species.ResultsIllumina sequencing results yielded 544,272,542 high-quality filtered reads. After de novo assembly using Trinity, 233,257 contigs were generated with an average length of 1,264 bp and an N50 length of 2,868 bp; 192,879 assembled transcripts and 150,111 assembled unigenes were obtained after clustering. A total of 93,668 assembled transcripts (66,692 assembled genes) with putative functions for protein domains were predicted based on InterProScan analysis. Based on similarity searches, 44,713 assembled transcripts and 25,319 assembled unigenes were annotated with at least one BLAST hit. A total of 21,262 assembled transcripts (11,947 assembled genes) were annotated with at least one well-defined Gene Ontology (GO) and 5,131 assembled transcripts (3,181 assembled unigenes) were assigned to 329 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The quantity of assembled unigenes and transcripts obtained from male and female mussels were similar but varied among the three studied tissues, with the highest numbers recorded in the gills, followed by the hepatopancreas, and then the adductor muscle. Multivariate analyses revealed strong tissue-specific patterns among the three different tissues, but not between sexes in terms of expression profiles for annotated genes in various GO terms, and genes associated with stress responses and degradation of xenobiotics. The expression profiles of certain selected genes in each tissue type were further validated using real-time quantitative polymerase chain reaction assays and a similar tissue-specific trend was seen.ConclusionsThe extensive sequence data generated from this study will provide a valuable molecular resource for facilitating environmental studies with P. viridis, and highlight the importance of tissue-specific approaches in the future.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-804) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

De novo transcriptome analysis of Perna viridis highlights tissue-specific patterns for environmental studies

Leung

Mak

et al. 2014

BMC Genomics

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“…GSTs are multifunctional isoenzymes involved in the cellular detoxification of reactive electrophilic compounds, regulation of redox homeostasis and innate immune responses in mussels (Wang et al, 2013). After 7 days of exposure to Cd-based QDs, GST activity increased in the mussel gills (1.7-fold) and digestive gland (1.6-fold) (p<0.05; Fig.…”

Section: Antioxidant Responsesmentioning

confidence: 93%

Tissue specific responses to cadmium-based quantum dots in the marine mussel Mytilus galloprovincialis

et al. 2015

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“…The GST gene family is poorly characterized in marine invertebrates, with a few genes being identified for instance in Unio tumidus, Laternula elliptica, Corbicula fluminea and M. galloprovincialis (Doyen et al, 2005(Doyen et al, , 2008Kim et al, 2009;Wang et al, 2013). As a result, few studies have addressed the functions of this family of enzymes at the gene level.…”

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of gill GSTs in Mytilus galloprovincialis from the North of Portugal and Galicia evidences variations at protein isoform level with a possible relation with water quality

Azevedo

Guzmán-Guillén

Martins

et al. 2015

Marine Environmental Research

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cDNA cloning and mRNA expression of four glutathione S-transferase (GST) genes from Mytilus galloprovincialis

Cited by 33 publications

References 23 publications

De novo transcriptome analysis of Perna viridis highlights tissue-specific patterns for environmental studies

De novo transcriptome analysis of Perna viridis highlights tissue-specific patterns for environmental studies

Tissue specific responses to cadmium-based quantum dots in the marine mussel Mytilus galloprovincialis

Proteomic profiling of gill GSTs in Mytilus galloprovincialis from the North of Portugal and Galicia evidences variations at protein isoform level with a possible relation with water quality

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