cDNA cloning, expression and antibacterial activity of lysozyme C in the blue shrimp (Litopenaeus stylirostris)

Protection of blue shrimp (Litopenaeus stylirostris) against the White Spot Syndrome Virus (WSSV) when injected with shrimp lysozyme

“…The enzymatic activity of the molecule does not appear to be necessary for this effect, and instead appears to be linked to the basic nature of the lysozyme molecule [35]. Compared with HEWL, the shrimp lysozyme has several novel structures; these are only found in the shrimp lysozyme [25,36,37]. These results suggest that the Lslzm protein could significantly improve shrimp protection against WSSV challenge and decrease the amount of viral infection by enhancing the innate immune response of shrimp to infection.…”

“…The recombinant protein was prepared as described for Escherichia coli BL21, which contains the Lslzm protein [25]. PCR was used to amplify a c-type lysozyme gene fragment that encoded the open reading frame.…”

Shrimp invertebrate lysozyme i-lyz: Gene structure, molecular model and response of c and i lysozymes to lipopolysaccharide (LPS)

“…Therefore, lysozymes attack Gram-positive bacteria. The c-lyz (similar to chicken hen egg white lysozyme) has been described in several crustaceans including the Pacific white (whiteleg) shrimp Litopenaeus vannamei [5], Kuruma prawn Marsupenaeus japonicus [6], Chinese shrimp Penaeus chinensis [7], giant tiger shrimp Penaeus monodon [8,9], banana shrimp Fenneropenaeus merguiensis [10], swimming crab Portunus trituberculatus [11] and the Pacific blue shrimp Litopenaeus stylirostris [12,13].…”

Molecular cloning and characterization of a novel c-type lysozyme gene in swimming crab Portunus trituberculatus

“…Moreover, recombinant lysozyme of kuruma shrimp Marsupenaeus japonicus displayed lytic activities against several Vibrio species, including V. penaeicida (a pathogenic bacteria to the kuruma shrimp) [23]. Recently, some c-type lysozymes have been cloned from shrimps, such as black tiger shrimp Penaeus monodon [17], pacific blue shrimp L. stylirostris [15] and banana prawn Fenneropenaeus merguiensis [16]. However, no lysozyme has been reported in crab.…”