cDNA microarray analysis of gene expression changes during pollination, pollen-tube elongation, fertilization, and early embryogenesis in rice pistils

Yoshida, Kaoru; Nakazono, Mikio; Fukuda, Hiroo; Demura, Taku; Watanabe, Masao

doi:10.1007/s00497-004-0238-4

Cited by 16 publications

(13 citation statements)

References 40 publications

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“…In remodeling of the cell wall in the tip region, a nonspecific esterase/lipase is secreted into the extracellular medium and functions in the process of wall softening (Yoshida et al, 2005); its down-regulation indicated decreased activity in the continuous process of cell wall softening and a consequent increase in cell wall rigidity. Our proteomic data further confirm that the cell wall of gymnosperm pollen tubes undergoes dramatic remodeling during the perturbation of Ca 2+ -CaM signaling.…”

Section: Proteins Involved In Polysaccharide Synthesis As the Tertiarmentioning

confidence: 99%

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

Chen

et al. 2008

Plant Physiology

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Ca 2+ -calmodulin (Ca 2+ -CaM) is a critical molecule that mediates cellular functions by interacting with various metabolic and signaling pathways. However, the protein expression patterns and accompanying serial cytological responses in Ca 2+ -CaM signaling deficiency remain enigmatic. Here, we provide a global analysis of the cytological responses and significant alterations in protein expression profiles after trifluoperazine treatment in Picea meyeri, which abrogates Ca 2+ -CaM signaling. Ninety-three differentially displayed proteins were identified by comparative proteomics at different development stages and were assigned to different functional categories closely related to tip growth machinery. The inhibition of Ca 2+ -CaM signaling rapidly induced an increase in extracellular Ca 2+ influx, resulting in dramatically increased cytosolic Ca 2+ concentrations and ultrastructural abnormalities in organelles as the primary responses. Secondary and tertiary alterations included actin filament depolymerization, disrupted patterns of endocytosis and exocytosis, and cell wall remodeling, ultimately resulting in perturbed pollen tube extension. In parallel with these cytological events, time-course experiments revealed that most differentially expressed proteins showed time-dependent quantitative changes (i.e. some signaling proteins and proteins involved in organelle functions and energy production changed first, followed by alterations in proteins related to cytoskeletal organization, secretory pathways, and polysaccharide synthesis). Taken together, Ca 2+ -CaM dysfunction induced serial cytological responses and temporal changes in protein expression profiles, indicating the pivotal role of Ca 2+ -CaM in the regulation of tip growth machinery.

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Section: Proteins Involved In Polysaccharide Synthesis As the Tertiarmentioning

confidence: 99%

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

Chen

et al. 2008

Plant Physiology

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“…The gene corresponding to clone TOBS008H02 encodes a ferredoxin 3 homolog and is specifically expressed at the reproductive organs, with a high expression level in ovaries. Interestingly, Yoshida et al (2005) also identified a rice pistil-specific gene encoding a ferredoxin III (Os3736; AC092557). Ferredoxins act as electron carriers in photosynthesis and play an important role in electron transfer processes.…”

Section: Real-time Rt-pcr Experiments and Validation Of The Macroarramentioning

confidence: 99%

“…Ferredoxins act as electron carriers in photosynthesis and play an important role in electron transfer processes. The pistil is a photosynthetic organ, and the elevation of ferredoxin transcript levels in pistils might be related to active electron transport in this organ (Yoshida et al, 2005) or involved in providing reductive agents to some specific metabolic process taking place in the reproductive organs.…”

Section: Real-time Rt-pcr Experiments and Validation Of The Macroarramentioning

confidence: 99%

“…Therefore, it is more likely that the differences encountered in gene expression in the female reproductive organs are related to the type of interaction between pollen and pistil in species with dry and wet stigmas. We have not done a detailed comparison with the rice pistil data sets (Yoshida et al, 2005;Li et al, 2007), since this is a monocotyledonous species and additional differences are expected in gene expression on top of the ones related to the strategy of pollen-pistil interaction.…”

Section: Wet and Dry Stigmas/styles Express Common As Well As Distincmentioning

confidence: 99%

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Analysis of theNicotiana tabacumStigma/Style Transcriptome Reveals Gene Expression Differences between Wet and Dry Stigma Species

Quiapim

Brito²,

Bernardes³

et al. 2008

Plant Physiology

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The success of plant reproduction depends on pollen-pistil interactions occurring at the stigma/style. These interactions vary depending on the stigma type: wet or dry. Tobacco (Nicotiana tabacum) represents a model of wet stigma, and its stigmas/styles express genes to accomplish the appropriate functions. For a large-scale study of gene expression during tobacco pistil development and preparation for pollination, we generated 11,216 high-quality expressed sequence tags (ESTs) from stigmas/ styles and created the TOBEST database. These ESTs were assembled in 6,177 clusters, from which 52.1% are pistil transcripts/ genes of unknown function. The 21 clusters with the highest number of ESTs (putative higher expression levels) correspond to genes associated with defense mechanisms or pollen-pistil interactions. The database analysis unraveled tobacco sequences homologous to the Arabidopsis (Arabidopsis thaliana) genes involved in specifying pistil identity or determining normal pistil morphology and function. Additionally, 782 independent clusters were examined by macroarray, revealing 46 stigma/style preferentially expressed genes. Real-time reverse transcription-polymerase chain reaction experiments validated the pistilpreferential expression for nine out of 10 genes tested. A search for these 46 genes in the Arabidopsis pistil data sets demonstrated that only 11 sequences, with putative equivalent molecular functions, are expressed in this dry stigma species. The reverse search for the Arabidopsis pistil genes in the TOBEST exposed a partial overlap between these dry and wet stigma transcriptomes. The TOBEST represents the most extensive survey of gene expression in the stigmas/styles of wet stigma plants, and our results indicate that wet and dry stigmas/styles express common as well as distinct genes in preparation for the pollination process.

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“…The transcriptional profiles of pollen (Becker et al, 2003;Twell, 2003, 2004;Pina et al, 2005; for review, see Becker and Feijó , 2007), embryo sac (Yu et al, 2005;Johnston et al, 2007;Jones-Rhoades et al, 2007;Steffen et al, 2007), pistil (Scutt et al, 2003;Tung et al, 2005), and embryo or seed developmental stages (Chen et al, 2001;Hennig et al, 2004;Yoshida et al, 2005, Le et al, 2010 were recently analyzed. More notably, recent progress in the isolation of Arabidopsis (Arabidopsis thaliana) gametes allowed genomewide expression profiling of purified sperm cells (Borges et al, 2008) as well as of isolated egg and central cells (Wuest et al, 2010).…”

mentioning

confidence: 99%

Whole Genome Analysis of Gene Expression Reveals Coordinated Activation of Signaling and Metabolic Pathways during Pollen-Pistil Interactions in Arabidopsis

et al. 2011

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Plant reproduction depends on the concerted activation of many genes to ensure correct communication between pollen and pistil. Here, we queried the whole transcriptome of Arabidopsis (Arabidopsis thaliana) in order to identify genes with specific reproductive functions. We used the Affymetrix ATH1 whole genome array to profile wild-type unpollinated pistils and unfertilized ovules. By comparing the expression profile of pistils at 0.5, 3.5, and 8.0 h after pollination and applying a number of statistical and bioinformatics criteria, we found 1,373 genes differentially regulated during pollen-pistil interactions. Robust clustering analysis grouped these genes in 16 time-course clusters representing distinct patterns of regulation. Coregulation within each cluster suggests the presence of distinct genetic pathways, which might be under the control of specific transcriptional regulators. A total of 78% of the regulated genes were expressed initially in unpollinated pistil and/or ovules, 15% were initially detected in the pollen data sets as enriched or preferentially expressed, and 7% were induced upon pollination. Among those, we found a particular enrichment for unknown transcripts predicted to encode secreted proteins or representing signaling and cell wall-related proteins, which may function by remodeling the extracellular matrix or as extracellular signaling molecules. A strict regulatory control in various metabolic pathways suggests that fine-tuning of the biochemical and physiological cellular environment is crucial for reproductive success. Our study provides a unique and detailed temporal and spatial gene expression profile of in vivo pollen-pistil interactions, providing a framework to better understand the basis of the molecular mechanisms operating during the reproductive process in higher plants.

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cDNA microarray analysis of gene expression changes during pollination, pollen-tube elongation, fertilization, and early embryogenesis in rice pistils

Cited by 16 publications

References 40 publications

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

Combined Proteomic and Cytological Analysis of Ca2+-Calmodulin Regulation inPicea meyeriPollen Tube Growth

Analysis of theNicotiana tabacumStigma/Style Transcriptome Reveals Gene Expression Differences between Wet and Dry Stigma Species

Whole Genome Analysis of Gene Expression Reveals Coordinated Activation of Signaling and Metabolic Pathways during Pollen-Pistil Interactions in Arabidopsis

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