cDNA sequencing and expression of Nramp1 (Slc11a1) in dogs phenotypically resistant or susceptible to visceral leishmaniasis

Bueno, Regiane Cristina Oliveira de Freitas; Neta, Alcina V. Carvalho; Xavier, Mariana N.; Oliveira, Raquel G.; Diniz, S.A.; Melo, Maria Norma; Santos, Renato L.

doi:10.1016/j.vetimm.2008.10.335

Cited by 11 publications

(7 citation statements)

References 36 publications

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“…A predisposition to CanL has been associated with the haplotype of T antigen epitope TAG-8-141 and with two single nucleotide polymorphisms (SNPs) (A4549G in intron 6 and C4859T in exon 8) located in the Slc11a1 gene in Boxer breed (Sanchez-Robert et al, 2005 ) and in different dog breeds (Sanchez-Robert et al, 2008 ), respectively. However, no significant differences in the expression of Slc11a1 gene between phenotypically resistant and susceptible dogs or between primary canine monocyte-derived macrophages from Leishmania -free dogs with higher or lower resistance to intracellular survival of the parasites were found in the studies performed by Bueno et al ( 2009 ) and Turchetti et al ( 2015 ). The presence of the beta chain allele of the dog leukocyte antigen DLA–DRB1 * 01502 has been associated with susceptibility to CanL (Quinnell et al, 2003b ) while the detection of SNPs 3, 4, 7 and 8 in the canine β -defensin-1 gene has been associated with susceptibility to L. infantum or L .…”

Section: Genetic Biomarkers Of Susceptibility To Leishmaniamentioning

confidence: 95%

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

Maia

Campino

2018

Front. Cell. Infect. Microbiol.

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Canine leishmaniosis (CanL) is a vector-borne disease caused by the protozoan Leishmania (Leishmania) infantum species [syn. L. (L.) infantum chagasi species in the Americas] which is transmitted by the bite of a female phlebotomine sand fly. This parasitosis is endemic and affect millions of dogs in Asia, the Americas and the Mediterranean basin. Domestic dogs are the main hosts and the main reservoir hosts for human zoonotic leishmaniosis. The outcome of infection is a consequence of intricate interactions between the protozoan and the immunological and genetic background of the host. Clinical manifestations can range from subclinical infection to very severe disease. Early detection of infected dogs, their close surveillance and treatment are essential to control the dissemination of the parasite among other dogs, being also a pivotal element for the control of human zoonotic leishmaniosis. Hence, the identification of biomarkers for the confirmation of Leishmania infection, disease and determination of an appropriate treatment would represent an important tool to assist clinicians in diagnosis, monitoring and in giving a realistic prognosis to subclinical infected and sick dogs. Here, we review the recent advances in the identification of Leishmania infantum biomarkers, focusing on those related to parasite exposure, susceptibility to infection and disease development. Markers related to the pathogenesis of the disease and to monitoring the evolution of leishmaniosis and treatment outcome are also summarized. Data emphasizes the complexity of parasite-host interactions and that a single biomarker cannot be used alone for CanL diagnosis or prognosis. Nevertheless, results are encouraging and future research to explore the potential clinical application of biomarkers is warranted.

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Section: Genetic Biomarkers Of Susceptibility To Leishmaniamentioning

confidence: 95%

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

Maia

Campino

2018

Front. Cell. Infect. Microbiol.

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“…Asymptomatic spontaneously-infected dogs have greater expression of genes encoding IFN-γ and tumour necrosis factor (TNF)-α and a lower parasite burden within lymph nodes, compared with symptomatic dogs with high parasite burdens in which there is greater expression of genes encoding the immunosuppressive cytokines IL-10 and transforming growth factor (TGF)-β [ 48 ]. In contrast, within the spleen there was no clear difference in IFN-γ, IL-4 and IL-12 gene expression between asymptomatic and symptomatic dogs [ 49 ], but splenic IL-10 mRNA expression was greater in symptomatic dogs [ 50 ]. There was no clear evidence for higher expression of IL-10 mRNA in the bone marrow or blood lymphocytes of infected versus uninfected or symptomatic versus asymptomatic dogs [ 51 , 52 ].…”

Section: The Immunology Of Canine Vector-borne Diseasesmentioning

confidence: 99%

“…the Ibizian hound and dogs raised in endemic areas) [ 59 ] has been explored by characterizing associations with genes of the canine MHC (the DLA system; specifically the DLA class II allele DLA-DRB1*01502) [ 60 ] and genes identified as associated with resistance in other species ( Slc11a1 encoding the natural resistance associated protein 1 [NRAMP1]) [ 61 ]. A recent study has failed to confirm sequence differences in Slc11a1 between symptomatic and asymptomatic naturally-infected dogs [ 49 ]. The most exciting current investigation of canine leishmaniosis is that which forms one of the work packages of the European Union-funded LUPA project http://www.eurolupa.org/ , in which susceptibility and resistance is being examined via GWAS studies of significant numbers of dogs with clinically well-characterized infection status and disease.…”

Section: The Immunology Of Canine Vector-borne Diseasesmentioning

confidence: 99%

The immunopathology of canine vector-borne diseases

Day

2011

Parasites Vectors

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The canine vector-borne infectious diseases (CVBDs) are an emerging problem in veterinary medicine and the zoonotic potential of many of these agents is a significant consideration for human health. The successful diagnosis, treatment and prevention of these infections is dependent upon firm understanding of the underlying immunopathology of the diseases in which there are unique tripartite interactions between the microorganism, the vector and the host immune system. Although significant advances have been made in the areas of molecular speciation and the epidemiology of these infections and their vectors, basic knowledge of the pathology and immunology of the diseases has lagged behind. This review summarizes recent studies of the pathology and host immune response in the major CVBDs (leishmaniosis, babesiosis, ehrlichiosis, hepatozoonosis, anaplasmosis, bartonellosis and borreliosis). The ultimate application of such immunological investigation is the development of effective vaccines. The current commercially available vaccines for canine leishmaniosis, babesiosis and borreliosis are reviewed.

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“…In the present study three previously described SNPs (SNP1 T151C, SNP2 Α180G και SNP3 G318A) in the promoter of the canine SLC11A1 gene were further analyzed in a case-control study of 148 dogs. Statistical analyses revealed a signifi cant association either for the SNP2 Α180G in heterozygosity (AG) or for the SNP3 G318A in homozygosity (AA) with susceptibility to CanL while in other studies all three of these SNPs were implicated in the control of infection [13,14,22].…”

Section: Discussionmentioning

confidence: 93%

Promoter Polymorphisms of the Canine SCL11A1 Gene are Correlated with Susceptibility to Canine Leishmaniosis

et al. 2020

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In enzootic areas the prevalence estimates of canine leishmaniosis are high whereas only a proportion of dogs exhibit the clinical disease, thus implying a role of host genetics. The type of the triggered immune response remains a crucial determining factor for the diverse outcome of this parasitosis. The Solute Carrier Family 11 member 1 (SLC11A1) is a protein, which plays a central role in macrophage function and is implicated in the regulation of the immune response. An extended study with 73 resistant and 75 susceptible to Leishmania dogs was conducted. A fragment of the promoter region of the canine SLC11A1 gene was amplified and digested providing the different genotypes for three previously recorded single-nucleotide polymorphisms (SNPs) (SNP1 T151C, SNP2 Α180G, SNP3 G318A) for each animal. Statistical analyses revealed that SNP2 Α180G in heterozygosity (AG) as well as SNP3 G318A in homozygosity (AA) are correlated with susceptibility to canine leishmaniosis.

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cDNA sequencing and expression of Nramp1 (Slc11a1) in dogs phenotypically resistant or susceptible to visceral leishmaniasis

Cited by 11 publications

References 36 publications

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

Biomarkers Associated With Leishmania infantum Exposure, Infection, and Disease in Dogs

The immunopathology of canine vector-borne diseases

Promoter Polymorphisms of the Canine SCL11A1 Gene are Correlated with Susceptibility to Canine Leishmaniosis

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