CDPKs and 14-3-3 Proteins: Emerging Duo in Signaling

Ormancey, Mélanie; Thuleau, Patrice; Mazars, Christian; Cotelle, Valérie

doi:10.1016/j.tplants.2016.11.007

Cited by 58 publications

(34 citation statements)

References 88 publications

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“…7 D ). Increasing evidence supports a role of these proteins in signal transduction during plant immune responses at diverse levels ( 55 , 56 ), and our data suggest that NRG1 is required for their up-regulation during plant immune responses. Another interesting finding is that the helper NLR ADR1, which, along with NRG1, is part of the RPW8-like CC domain subclass, is up-regulated only in the nrg1 mutant upon XopQ expression ( Fig.…”

Section: Discussionsupporting

confidence: 71%

NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in Nicotiana benthamiana

Seong

Thomazella

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

193

259

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SignificancePlants employ nucleotide-binding leucine-rich repeat (NLR) immune receptors to recognize pathogen effectors and to activate effector-triggered immunity (ETI). The Toll/IL-1 receptor-NLR (TNL) protein (Roq1) recognizes the effectors XopQ and HopQ1 in an Enhanced Disease Susceptibility 1 (EDS1)-dependent way in Nicotiana benthamiana. Interestingly, we found that the coiled-coil NLR protein N requirement gene 1 (NRG1) is required for activation of ETI by the TNLs Roq1 and Recognition of Peronospora parasitica 1. NRG1 interacts with EDS1 and acts downstream of Roq1 and EDS1 to mediate XopQ/HopQ1-triggered ETI. In addition, Roq1, EDS1, and NRG1 mediate XopQ-triggered transcriptional changes in N. benthamiana and regulate resistance to Xanthomonas and Pseudomonas species that carry the effectors XopQ or HopQ1. This study suggests that NRG1 may be a conserved key component in TNL-mediated signaling pathways.

show abstract

Section: Discussionsupporting

confidence: 71%

NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in Nicotiana benthamiana

Seong

Thomazella

et al. 2018

Proc. Natl. Acad. Sci. U.S.A.

193

259

View full text Add to dashboard Cite

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“…A greater number of dual localized proteins were in larger complexes in the membrane fraction compared with the cytosol. GRFs function as signaling proteins ( 70 ) and multiple isoforms were identified in this study. In the cytosol, all the GRFs coeluted with a R app of ∼3, but the membrane-associated GRFs showed specificity with unique peaks.…”

Section: Resultsmentioning

confidence: 98%

Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling

McBride

Chen

Reick

et al. 2017

Molecular & Cellular Proteomics

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Membrane-associated proteins are required for essential processes like transport, organelle biogenesis, and signaling. Many are expected to function as part of an oligomeric protein complex. However, membrane-associated proteins are challenging to work with, and large-scale data sets on the oligomerization state of this important class of proteins is missing. Here we combined cell fractionation of Arabidopsis leaves with nondenaturing detergent solubilization and LC/MS-based profiling of size exclusion chromatography fractions to measure the apparent masses of >1350 membrane-associated proteins. Our method identified proteins from all of the major organelles, with more than 50% of them predicted to be part of a stable complex. The plasma membrane was the most highly enriched in large protein complexes compared with other organelles. Hundreds of novel protein complexes were identified. Over 150 proteins had a complicated localization pattern, and were clearly partitioned between cytosolic and membrane-associated pools. A subset of these dual localized proteins had oligomerization states that differed based on localization. Our data set is an important resource for the community that includes new functionally relevant data for membrane-localized protein complexes that could not be predicted based on sequence alone. Our method enables the analysis of protein complex localization and dynamics, and is a first step in the development of a method in which LC/MS profile data can be used to predict the composition of membrane-associated protein complexes.

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“…Thus, CaM has an effect on transmitting the calcium signals to downstream proteins and regulating plant growth and development. CDPKs, which are calcium sensors, are also implicated in the growth of plants, such as pollen development and gravitropism, and their response to environment stresses, such as pathogens (Ormancey et al, 2017 ). The MAPK cascade consists of interlinked MAPK, MAPKK, and MAPKKK and such cascades play important roles in signal transduction of plant hormone, biotic stress and abiotic stress (Wang et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and Differential Expression Profiling Analysis of the Mechanism of Ca2+ Regulation in Peanut (Arachis hypogaea) Pod Development

et al. 2017

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Calcium not only serves as a necessary nutrient for plant growth but also acts as a ubiquitous central hub in a large number of signaling pathways. Free Ca2+ deficiency in the soil may cause early embryo abortion, which eventually led to abnormal development of peanut pod during the harvest season. To understand the mechanisms of Ca2+ regulation in pod development, transcriptome analysis of peanut gynophores and pods was performed by comparing the treatments between free Ca2+ sufficiency and free Ca2+ deficiency using Illumina HiSeq™ 2000. 9,903,082,800 nt bases are generated totally. After assembly, the average length of 102,819 unigenes is 999 nt, N50 is 1,782 nt. RNA-seq based gene expression profilings showed a large number of genes at the transcriptional level changed significantly between the aerial pegs and underground swelling pods under free Ca2+ sufficienct or deficiency treatments, respectively. Genes encoding key members of Ca2+ signaling transduction pathway, enzymes for hormone metabolism, cell division and growth, transcriptional factor as well as embryo development were highlighted. This information provides useful information for our further study. The results of digital gene expression (DGE) indicated that exogenous calcium might contribute to the development of peanut pod through its signal transduction pathway, meanwhile, promote the normal transition of the gynophores to the reproductive development.

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CDPKs and 14-3-3 Proteins: Emerging Duo in Signaling

Cited by 58 publications

References 88 publications

NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in Nicotiana benthamiana

NRG1 functions downstream of EDS1 to regulate TIR-NLR-mediated plant immunity in Nicotiana benthamiana

Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling

Transcriptome and Differential Expression Profiling Analysis of the Mechanism of Ca2+ Regulation in Peanut (Arachis hypogaea) Pod Development

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