CdTe‐Based QDs: Preparation, Cytotoxicity, and Tumor Cell Death by Targeting Transferrin Receptor

Gallo, Juan; Garcı́a, Isabel; Genicio, Nuria; Penadés, Soledad

doi:10.1002/ppsc.201300291

Cited by 5 publications

(4 citation statements)

References 47 publications

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“…The gold NPs were characterized by DCS and EM. The synthesis of mercaptopropionic acid (MPA)-protected CdTe QDs was performed following the procedure reported by Penadeś et al 47 The QDs were purified by precipitation with acetone. Finally, the QDs were separated by centrifugation and dialyzed 48 h against PBS buffer.…”

Section: Methodsmentioning

confidence: 99%

Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles

et al. 2017

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Biomolecules adsorbed on nanoparticles are known to confer a biological identity to nanoparticles, mediating the interactions with cells and biological barriers. However, how these molecules are presented on the particle surface in biological milieu remains unclear. The central aim of this study is to identify key protein recognition motifs and link them to specific cell-receptor interactions. Here, we employed an immuno-mapping technique to quantify epitope presentations of two major proteins in the serum corona, low-density lipoprotein and immunoglobulin G. Combining with a purpose-built receptor expression system, we show that both proteins present functional motifs to allow simultaneous recognition by low-density lipoprotein receptor and Fc-gamma receptor I of the corona. Our results suggest that the "labeling" of nanoparticles by biomolecular adsorption processes allows for multiple pathways in biological processes in which they may be "mistaken" for endogenous objects, such as lipoproteins, and exogenous ones, such as viral infections.

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Section: Methodsmentioning

confidence: 99%

Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles

et al. 2017

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“…The synthesis of mercaptopropionic acid protected CdTe quantum dots was performed following the procedure reported by Penades et al 50. A solution of 0.2 mmol of CdCl 2 in 40 ml of deoxygenated water was mixed with 0.34 mmol of MPA.…”

Section: Methodsmentioning

confidence: 99%

In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry

et al. 2016

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Nanoparticles interacting with, or derived from, living organisms are almost invariably coated in a variety of biomolecules presented in complex biological milieu, which produce a bio-interface or ‘biomolecular corona' conferring a biological identity to the particle. Biomolecules at the surface of the nanoparticle–biomolecule complex present molecular fragments that may be recognized by receptors of cells or biological barriers, potentially engaging with different biological pathways. Here we demonstrate that using intense fluorescent reporter binders, in this case antibodies bound to quantum dots, we can map out the availability of such recognition fragments, allowing for a rapid and meaningful biological characterization. The application in microfluidic flow, in small detection volumes, with appropriate thresholding of the detection allows the study of even complex nanoparticles in realistic biological milieu, with the emerging prospect of making direct connection to conditions of cell level and in vivo experiments.

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“…A range of probes were used to observe and measure the extent of cellular poration: propidium iodide was used initially for proof-of-concept work, while characterization of the pore structure and the dynamics of payload delivery was accomplished using CdTe quantum dots (QDs) of a range of diameters, between approximately 3 nm7nm [12,13]. Propidium iodide penetration was observed throughout targeted cells almost immediately, but QD experiments revealed far more interesting behavior.…”

Section: Resultsmentioning

confidence: 99%

A Novel, All-Optical Tool for Controllable and Non-Destructive Poration of Cells with Single-Micron Resolution

Casey

Wylie

Gallo

et al. 2015

Optics in the Life Sciences

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Abstract:We demonstrate controllable poration within ≈1 µm regions of individual cells, mediated by a near-IR laser interacting with thin-layer amorphous silicon substrates. This technique will allow new experiments in single-cell biology, particularly in neuroscience.As our understanding of the fundamental mechanistic processes underpinning biology expands, so does the need for high-precision tools to allow the dissection of the heterogeneity and stochastic processes that dominate at the single-and sub-cellular level. Here, we demonstrate a highly controllable and reproducible optical technique for inducing poration within specific regions of a target cell's plasma membrane, permitting localized delivery of payloads, depolarization and lysis experiments to be conducted in unprecedented detail. Experiments support a novel mechanism for the process, based upon a thermally-induced change triggered by the interactions of a near-IR laser with a biocompatible thin film substrate at powers substantially below that used in standard optoporation experiments.

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CdTe‐Based QDs: Preparation, Cytotoxicity, and Tumor Cell Death by Targeting Transferrin Receptor

Cited by 5 publications

References 47 publications

Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles

Identification of Receptor Binding to the Biomolecular Corona of Nanoparticles

In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry

A Novel, All-Optical Tool for Controllable and Non-Destructive Poration of Cells with Single-Micron Resolution

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