Ce3+-induced exopolysaccharide production by Bradyrhizobium sp. MAFF211645

Fitriyanto, Nanung Agus; Nakamura, Masashi; Muto, Shuji; Katô, Kôji; Yabe, Tomio; Iwama, Tetsuo; Kawai, Kazuhiro; Pertiwiningrum, Ambar

doi:10.1016/j.jbiosc.2010.09.008

Cited by 38 publications

(28 citation statements)

References 29 publications

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“…Several recent studies suggest that in methano- and methylotrophic bacteria, the REE switch might affect more genes than only the genes needed for the periplasmic oxidation system itself ( 16 , 22 , 43 ). Reports on physiological consequences are, however, inconsistent as some studies found no effects ( 13 , 16 , 23 , 44 ), whereas other studies reported a stimulating effect on biofilm formation, growth rates, and overall yields in the presence of REE ( 43 , 45 ). We think it is not unlikely that additional REE-mediated regulatory effects also exist in P. putida KT2440 in a context-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440

Wehrmann

Berthelot

Billard

et al. 2018

mSphere

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The function of lanthanides for methanotrophic and methylotrophic bacteria is gaining increasing attention, while knowledge about the role of rare earth elements (REEs) in nonmethylotrophic bacteria is still limited. The present study investigates the recently described differential expression of the two PQQ-EDHs of P. putida in response to lanthanides. We demonstrate that a specific TCS is crucial for their inverse regulation and provide evidence for a dual regulatory function of the LuxR-type response regulator involved. Thus, our study represents the first detailed characterization of the molecular mechanism underlying the REE switch of PQQ-EDHs in a nonmethylotrophic bacterium and stimulates subsequent investigations for the identification of additional genes or phenotypic traits that might be coregulated during REE-dependent niche adaptation.

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Section: Discussionmentioning

confidence: 99%

The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440

Wehrmann

Berthelot

Billard

et al. 2018

mSphere

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“…Lanthanides induced exopolysaccharide production in Bradyrhizobium sp. strain MAFF211645 ( 41 ). We did not recognize any difference in the colony morphology, motility, and biofilm formation ( Fig.…”

Section: Discussionmentioning

confidence: 99%

Lanthanide-Dependent Regulation of Methylotrophy in Methylobacterium aquaticum Strain 22A

Masuda

Suzuki

Fujitani

et al. 2018

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Lanthanides have been considered unimportant for biological processes. In methylotrophic bacteria, however, a methanol dehydrogenase (MDH) encoded by xoxF was recently found to be lanthanide dependent, while the classic-type mxaFI is calcium dependent. XoxF-type MDHs are more widespread in diverse bacterial genera, suggesting their importance for methylotrophy. Methylobacterium species, representative methylotrophic and predominating alphaproteobacteria in the phyllosphere, contain both types and regulate their expression depending on the availability of lanthanides. RNA-seq analysis showed that the regulation takes place not only for MDH genes but also the subsequent formaldehyde oxidation pathways and respiratory chain, which might be due to the direct oxidation of methanol to formate by XoxF. In addition, a considerable number of genes of unknown function, including AT-rich genes, were found to be upregulated in the presence of lanthanides. This study provides first insights into the specific reaction of methylotrophic bacteria to the presence of lanthanides, emphasizing the biological relevance of this trace metal.

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“…Several recent studies suggest that in methano- and methylotrophic bacteria, the REE switch might affect more genes than only those needed for the periplasmic oxidation system itself (Gu and Semrau, 2017; Good et al, 2018; Masuda et al, 2018). Reports on physiological consequences are, however, inconsistent as some studies found no effects (Nakagawa et al, 2012; Chu and Lidstrom, 2016; Vu et al, 2016; Masuda et al, 2018), whereas other studies reported a stimulating effect on biofilm formation, growth rates, and overall yields in the presence of REE (Fitriyanto et al, 2011b; Nakamura, et al, 2011; Good et al, 2018). We think it is not unlikely that additional REE-mediated regulatory effects do also exist in P. putida KT2440 in a context-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

The PedS2/PedR2 two-component system is crucial for the rare earth element switch in Pseudomonas putida KT2440

Wehrmann¹,

Berthelot

Billard

et al. 2018

Preprint

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In Pseudomonas putida KT2440, two pyrroloquinoline quinone-dependent ethanol dehydrogenases (PQQ-EDHs) are responsible for the periplasmic oxidation of a broad variety of volatile organic compounds (VOCs). Depending on the availability of rare earth elements (REEs) of the lanthanide series (Ln3+), we have recently described that the transcription of the genes encoding the Ca2+-utilizing enzyme PedE and the Ln3+-utilizing enzyme PedH are inversely regulated. With adaptive evolution experiments, site-specific mutations, transcriptional reporter fusions, and complementation approaches, we herein demonstrate that the PedS2/PedR2 (PP_2671/PP_2672) two-component system (TCS) plays a central role in the observed REE-mediated switch of PQQ-EDHs in P. putida. We provide evidence that in the absence of lanthanum (La3+), the sensor histidine kinase PedS2 phosphorylates its cognate LuxR-type response regulator PedR2, which in turn not only activates pedE gene transcription but is also involved in repression of pedH. Our data further suggests that the presence of La3+ lowers kinase activity of PedS2, either by the direct binding of the metal ions to the periplasmic region of PedS2 or by an uncharacterized indirect interaction, leading to reduced levels of phosphorylated PedR2. Consequently, the fading pedE expression and concomitant alleviation of pedH repression causes – in conjunction with the transcriptional activation of the pedH gene by a yet unknown regulatory module – the Ln3+-dependent transition from PedE to PedH catalysed oxidation of alcoholic VOCs.IMPORTANCEThe function of lanthanides for methano- and methylotrophic bacteria is gaining increasing attention, while knowledge about the role of rare earth elements (REEs) in non-methylotrophic bacteria is still limited. The present study investigates the recently described differential expression of the two PQQ-EDHs of P. putida in response to lanthanides. We demonstrate that a specific TCS is crucial for their inverse regulation and provide evidence for a dual regulatory function of the LuxR-type response regulator involved. Thus, our study represents the first detailed characterization of the molecular mechanism underlying the REE switch of PQQ-EDHs in a non-methylotrophic bacterium and stimulates subsequent investigations for the identification of additional genes or phenotypic traits that might be co-regulated during REE-dependent niche adaptation.

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Ce3+-induced exopolysaccharide production by Bradyrhizobium sp. MAFF211645

Cited by 38 publications

References 29 publications

The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440

The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440

Lanthanide-Dependent Regulation of Methylotrophy in Methylobacterium aquaticum Strain 22A

The PedS2/PedR2 two-component system is crucial for the rare earth element switch in Pseudomonas putida KT2440

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