CEA, AFP and CA 19-9 analysis in peritoneal fluid to differentiate causes of ascites formation

Kaleta, Erin J.; Tolan, Nicole V.; Ness, Karl A.; O’Kane, Dennis J.; Algeciras‐Schimnich, Alicia

doi:10.1016/j.clinbiochem.2013.02.010

Cited by 31 publications

(24 citation statements)

References 14 publications

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“…Other reports (Tuzun et al, 2009;Kaleta et al, 2013) showed that the diagnostic sensitivity, specificity and accuracy of ascites CEA were 31%, 90% and >98%, respectively. AFP, as kind of serum glycoprotein synthesized by liver and yolk sac in the early stage of fetal development, will disappear gradually with the ages, and it is the most classical marker for hepatic cancers.…”

Section: Discussionmentioning

confidence: 99%

“…AFP, as kind of serum glycoprotein synthesized by liver and yolk sac in the early stage of fetal development, will disappear gradually with the ages, and it is the most classical marker for hepatic cancers. A research (Kaleta et al, 2013) indicated that AFP was obviously higher in malignant ascites than in benign ascites, with diagnostic sensitivity and specificity being 17% and 95%, respectively. CA125, as a kind of macromolecular poly-glycoprotein, will increase evidently in the blood of patients when the cellular structures are damaged and changed in malignant tissues.…”

Section: Discussionmentioning

confidence: 99%

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Tumor Markers in Serum and Ascites in the Diagnosis of Benign and Malignant Ascites

Zhu¹,

Ling²,

Wei³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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Objective: To evaluate the values of 4 tumor markers in serum and ascites and their ascites/serum ratios in the identification and diagnosis of benign and malignant ascites. Materials and Methods: A total of 76 patients were selected as subjects and divided into malignant ascites group (45 cases) and benign ascites group (31 cases). Samples of ascites and serum of all hospitalized patients were collected before treatment. The levels of carcinoembryonic antigen (CEA), alpha fetoprotein (AFP), cancer antigen 125 (CA125) and carbohydrate antigen 19-9 (CA19-9) were detected by chemiluminescence (CLIA) . Results: CEA, AFP and CA19-9 in both serum and ascites as well as CA125 in ascites were evidently higher in the malignant ascites group than in the benign ascites group (P<0.01). Malignant ascites was associated with elevated ascites/serum ratios for AFP and CA125 (P<0.01). The areas under receiver operating characteristic (AUROCs) of CEA and CA125 in ascites and the ratios of ascites/serum of AFP, CEA, CA125 and CA19-9 were all >0.7, suggesting certain values, while those of ascites CA19-9 and serum CEA were 0.697 and 0.629 respectively, indicating low accuracy in the identification and diagnosis of benign and malignant ascites. However, the AUROCs of the remaining indexes were <0.5, with no value for identification and diagnosis. Compared with single index, the sensitivity of combined detection increased significantly (P<0.05), in which the combined detection of CEA, CA19-9 and CA125 in ascites as well as the ratio of ascites/serum of CEA, CA19-9, CA125 and AFP had the highest sensitivity (98.4%) but with relevantly low specificity. Both sensitivity and specificity of combined detection should be comprehensively considered so as to choose the most appropriate index. Conclusions: Compared with single index, combined detection of tumor markers in serum and ascites can significantly improve the diagnostic sensitivity and specificity.Keywords: CEA -alpha fetoprotein -CA125 -carbohydrate antigen 19-9 -benign and malignant ascites -diagnosis

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tumor Markers in Serum and Ascites in the Diagnosis of Benign and Malignant Ascites

Zhu¹,

Ling²,

Wei³

et al. 2015

Asian Pacific Journal of Cancer Prevention

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“…From previous studies, methods such as relative lymphocyte count, MA tumor markers, and telomerase activity were examined. [22][23][24][25][26] All of these new methods have their own advantages; however, cytology is still the recognized gold standard in clinical practice. Cytology provides direct insight into the cell components of MA.…”

Section: Discussionmentioning

confidence: 99%

Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification

Zeng

Peng

Yuan

et al. 2015

IJN

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Purpose The aims of this study are to establish a new method for simultaneously detecting the interactions between cancer cells and immunocytes in malignant ascites (MA) and to propose a new model for MA classification. Methods A quantum dot (QD)-based multiplexed imaging technique was developed for simultaneous in situ imaging of cancer cells, lymphocytes, and macrophages. This method was first validated in gastric cancer tissues, and then was applied to MA samples from 20 patients with peritoneal carcinomatosis from gastrointestinal and gynecological origins. The staining features of MA and the interactions between cancer cells and immunocytes in the ascites were further analyzed and correlated with clinical features. Results The QD-based multiplexed imaging technique was able to simultaneously show gastric cancer cells, infiltrating macrophages, and lymphocytes in tumor tissue, and the technique revealed the distinctive features of the cancer tumor microenvironment. When this multiplexed imaging protocol was applied to MA cytology, different features of the interactions and quantitative relations between cancer cells and immunocytes were observed. On the basis of these features, MA could be classified into immunocyte-dominant type, immunocyte-reactive type, cancer cell-dominant type, and cell deletion type; the four categories were statistically different in terms of the ratio of cancer cells to immunocytes ( P <0.001). Moreover, in the MA, the ratio of cancer cells to immunocytes was higher for patients with gynecological and gastric cancers than for those with colorectal cancer. Conclusion The newly developed QD-based multiplexed imaging technique was able to better reveal the interactions between cancer cells and immunocytes. This advancement allows for better MA classification and, thereby, allows for treatment decisions to be more individualized.

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“…8 The specificity of this method is very high, but it has low sensitivity (40-60%) due to the lack of cell exfoliation, which is common to all malignancies. 5 This low sensitivity sometimes leads to invasive procedures, including laparoscopy, to acquire peritoneal tissues. hydronephrosis.…”

Section: Introductionmentioning

confidence: 99%

“…1 Ascites fluid analysis by paracentesis can provide useful information for determining the cause of ascites. 2,3 Several potential parameters for the diagnosis of malignant ascites have been evaluated to date, including protein levels in ascites, ascites/serum concentration ratio of protein (protein A/S), lactate dehydrogenase (LDH) levels in ascites, ascites/serum concentration ratio of LDH (LDH A/S), 4 carbohydrate antigen 19-9 in ascites, 5 serum-ascites albumin gradient, 6 fibronectin in ascites, and cholesterol in ascites. 7 However, until now, no parameter was able to completely differentiate the cause of malignant ascites; currently, the gold standard for diagnosing malignant ascites is the presence of tumor cells in ascites.…”

Section: Introductionmentioning

confidence: 99%

Diagnostic Value of Carcinoembryonic Antigen in Ascites for Colorectal Cancer with Peritoneal Carcinomatosis

Song

Choi

Kim

et al. 2018

Korean J Gastroenterol

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CEA, AFP and CA 19-9 analysis in peritoneal fluid to differentiate causes of ascites formation

Cited by 31 publications

References 14 publications

Tumor Markers in Serum and Ascites in the Diagnosis of Benign and Malignant Ascites

Tumor Markers in Serum and Ascites in the Diagnosis of Benign and Malignant Ascites

Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification

Diagnostic Value of Carcinoembryonic Antigen in Ascites for Colorectal Cancer with Peritoneal Carcinomatosis

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CEA, AFP and CA 19-9 analysis in peritoneal fluid to differentiate causes of ascites formation

Cited by 31 publications

References 14 publications

Tumor Markers in Serum and Ascites in the Diagnosis of Benign and Malignant Ascites

Tumor Markers in Serum and Ascites in the Diagnosis of Benign and Malignant Ascites

Quantum dot-based multiplexed imaging in&nbsp;malignant ascites: a new model for malignant ascites classification

Diagnostic Value of Carcinoembryonic Antigen in Ascites for Colorectal Cancer with Peritoneal Carcinomatosis

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Quantum dot-based multiplexed imaging in malignant ascites: a new model for malignant ascites classification