Cedrus libani tar prompts reactive oxygen species toxicity and DNA damage in colon cancer cells

Temiz, Ebru; Eği, Kadir; Koyuncu, İsmail; Yüksekdağ, Özgür; Kurt, Yusuf; Tiken, Murat; Akmeşe, Şükrü

doi:10.1007/s11033-022-07631-7

Cited by 4 publications

(1 citation statement)

References 51 publications

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“…Research over the past decade has highlighted that cancer is a metabolic disease due to different levels of mitochondrial dysfunctions and metabolic changes, [28,50] with mitochondria being essential in regulating cellular energy production, intrinsic-cell death signals, and generating ROS. [51,52] The main metabolic changes in the mitochondria of tumor cells include altered aerobic glycolysis, [53] irregular pH gradient, [54][55][56] increased ROS production, [57] and impaired enzyme activities. [28] The impact of JMBE on cellular effects, organelle integrity, and ROS was analyzed using specific staining techniques in flow cytometry.…”

Section: Discussionmentioning

confidence: 99%

A mixed‐apoptotic effect of Jurinea mesopotamica extract on prostate cancer cells: a promising source for natural chemotherapeutics

Koyuncu,

Temiz,

Seker

et al. 2024

Chemistry & Biodiversity

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This research investigates the potential use of Jurinea mesopotamica Hand.‐Mazz. (Asteraceae) in cancer treatment. In this study, a plant extract was prepared using all parts of J. mesopotamica, and its effect on the proliferation of cancer and normal cells was tested using the MTT method. It was found to have a selective cytotoxic effect on prostate cancer cells, with the lowest IC50 (half‐maximal inhibitory concentration) of 10µg/mL found in the butanol extract (JMBE). The extract suppressed the proliferation of prostate cancer cells (67%), disrupted organelle integrity (49%), increased reactive oxidative stress (66%), and triggered cell death (51%). In addition, apoptotic gene expressions and protein levels increased, and the profile of amino acids related to energy metabolism was elevated. Based on LC‐MS/MS results, the plant contained higher levels of flavonoids, including isoquercitrin, cosmosiin, astragalin, nicotiflorin, luteolin, and apigenin. These results suggest that J. mesopotamica has a selective effect on prostate cancer due to its high flavonoid content and might be a promising natural alternative for cancer treatment.

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Section: Discussionmentioning

confidence: 99%

A mixed‐apoptotic effect of Jurinea mesopotamica extract on prostate cancer cells: a promising source for natural chemotherapeutics

Koyuncu,

Temiz,

Seker

et al. 2024

Chemistry & Biodiversity

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Targeting Yes‐Associated Protein (YAP) in Breast Cancer: In Silico Molecular Dynamics, Luminescence‐Based In Vitro, and In Vivo Validation of Rauvolfia tetraphylla‐Derived Inhibitors

Balavaishnavi,

Kamaraj,

Nithya

et al. 2024

Luminescence

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The study aims to elucidate the pharmacological mechanism of Rauvolfia tetraphylla against breast cancer through a comprehensive, multi‐faceted approach. This includes molecular docking, molecular dynamics, and experimental validation. Initial screening via ADME analysis and network pharmacology identified key compounds and potential targets. Protein–protein interaction (PPI) network analysis pinpointed Yes‐associated protein‐1 (YAP) as a crucial target. Molecular docking revealed that three compounds—ajmaline, reserpine, and serpentine—exhibited strong binding affinities with YAP, with scores of −6.5 to −6.7 kcal/mol. Molecular dynamics simulations were conducted to assess the stability of these interactions further. Experimental validation showed R. tetraphylla inhibited breast cancer cell proliferation, with an IC50 of 348.69 μg/mL, while demonstrating cytoprotective effects on Vero cells (IC50: 1056.23 μg/mL). Migration assays indicated an 88.5% reduction in cell migration, and increased ROS levels signaled elevated stress in cancer cells. Apoptosis was confirmed by AO/EtBr staining. In vivo validation in a DMBA‐induced mouse model confirmed significant tumor growth inhibition, supported by changes in YAP expression and histopathological analysis. These findings highlight R. tetraphylla as a promising therapeutic candidate against breast cancer, offering insights into its mechanisms and potential for future drug development and clinical applications.

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Metabolic reprogramming in sepsis-associated acute kidney injury: insights from lipopolysaccharide-induced oxidative stress and amino acid dysregulation

Turk,

Temiz,

Koyuncu

2024

Mol Biol Rep

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Cedrus libani tar prompts reactive oxygen species toxicity and DNA damage in colon cancer cells

Cited by 4 publications

References 51 publications

A mixed‐apoptotic effect of Jurinea mesopotamica extract on prostate cancer cells: a promising source for natural chemotherapeutics

A mixed‐apoptotic effect of Jurinea mesopotamica extract on prostate cancer cells: a promising source for natural chemotherapeutics

Targeting Yes‐Associated Protein (YAP) in Breast Cancer: In Silico Molecular Dynamics, Luminescence‐Based In Vitro, and In Vivo Validation of Rauvolfia tetraphylla‐Derived Inhibitors

Metabolic reprogramming in sepsis-associated acute kidney injury: insights from lipopolysaccharide-induced oxidative stress and amino acid dysregulation

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