Celastrus paniculatus Willd. mitigates t-BHP induced oxidative and apoptotic damage in C2C12 murine muscle cells

Kandikattu, Hemanth Kumar; Venuprasad, M. P.; Jayashree, G.V.; Rachitha, P.; Krupashree, K.; Pal, Ajay; Khanum, Farhath

doi:10.1007/s10616-014-9733-0

Cited by 14 publications

(8 citation statements)

References 39 publications

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“…Lead, a ubiquitous heavy metal and a known toxicant also produces adverse effects on the brain via increased production of reactive oxygen species (ROS) and causes oxidative stress. 22 This, in turn, results in oxidative stress at the cellular level causing a reduction in the sarcolemmal calcium pump and Na + K + ATPase intracellular Ca 2+ overload. Lead can substitute for calcium ions (Ca 2+ ) and picomolar concentrations of Pb 2+ can replace micromolar concentrations of Ca 2+ in a protein kinase C (PKC) enzyme assay, a calcium-dependent process.…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of Celastrus paniculatus Seed Extract against Lead Acetate Induced Nephrotoxicity in Wistar Rats

Balaji¹,

Vijayakumar²,

Sivanesan³

et al. 2021

IJPER

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Objectives:The plant Celastrus paniculatus is used in the traditional medical practices of India to treat a plethora of diseases. Earlier research on the plant revealed several biological properties and interesting bioactive compounds with significant medicinal uses. Materials and Methods: In this study, the ethanolic extract of the seeds of the plant (EECP) has been investigated against lead acetate (LA) induced nephrotoxicity in Wistar rats. Thirty rats were divided into five groups (n=6) wherein group 1 that contained normal animals served as control while group 2 received LA (30mg/kgb.w/day, p.o.). Animals in groups 3 -5 received respectively the standard drug N-acetylcysteine (NAC, 200mg/kg b.w/day, p.o.) and EECP in two doses (400and 800mg/kgb.w/day, p.o.) together with LA (30mg/kg b.w/day, p.o.) for 28 consecutive days.On day 29, all the animals were sacrificed and the blood and kidney were collected for analysis. Results and Conclusion: LA significantly decreased the level of catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), reduced glutathione (GR), glutathione S-transferase (GST) and glutathione (GSH) and increased the level of hydrogen peroxide (H 2 O 2 ), hydroxyl radicals (OH -) and lipid peroxidation (LPO) as compared to those in control rats. Administration of EECP especially 800mg/kg b.w. significantly downregulated the serum urea, creatinine and KIM 1 levels and also the level of oxidative stress markers when compared to the LA group. Histological and immunohistochemistry (KIM 1) study showed a significant change in the cytoarchitecture of the renal tissue thereby revealing the pro-active role of C. paniculatus seeds in nephrotoxicity.

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Section: Discussionmentioning

confidence: 99%

Protective Effect of Celastrus paniculatus Seed Extract against Lead Acetate Induced Nephrotoxicity in Wistar Rats

Balaji¹,

Vijayakumar²,

Sivanesan³

et al. 2021

IJPER

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“…Pre‐treatment with the extract also controlled the responsible markers including superoxide dismutase and catalase amount facilitated by protein expression. Anti‐apoptotic responses by regulation of cytochrome‐C and thermal shock protein‐70 expression were seen that resulted in 43% inhibition of DNA distortion in muscle cells against t‐BHP (Kumar et al, 2015).…”

Section: Central Nervous System Activitiesmentioning

confidence: 99%

“…The extract confirmed its potential via restoration of mitochondrial membrane and diminished the production of reactive oxygen species and decreased lipid per oxidation.Pre-treatment with the extract also controlled the responsible markers including superoxide dismutase and catalase amount facilitated by protein expression. Anti-apoptotic responses by regulation of cytochrome-C and thermal shock protein-70 expression were seen that resulted in 43% inhibition of DNA distortion in muscle cells against t-BHP (Kumar et al, 2015).…”

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confidence: 99%

An extensive review on phytochemistry and pharmacological activities of Indian medicinal plantCelastrus paniculatusWilld

Nagpal

Garg

Arora

et al. 2022

Phytotherapy Research

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Celastrus paniculatus is a traditional herb belonging to the family Celastraceae and is widely used for a number of medicinal activities in the Indian Unani and Ayurvedic systems. In this study, the extensive literature search was carried out on phytochemistry, ethnobotanical uses and pharmacological activities of C. paniculatus (Willd.) in various scientific databases as well as patents. Research on phytochemical investigation has shown the presence of monoterpenes (linalool, α‐terpinyl acetate, nerol acetate), sesqueterpene esters (such as malkanguniol, malkangunin, valerenal, globulol, viridiflorol, cubenol and agarofuran derivatives), diterpenoids (such as phytone, isophytol), triterpenoids (such as lupeol, pristimerin, paniculatadiol, zeylasteral, zeylasterone, β‐amyrin, squalene), alkaloids (celapanin, celapanigin, celapagin, paniculatine, celastrine, maymyrsine), fatty acids, steroids (β‐sitosterol, carpesterol benzoate), flavonoids (paniculatin), benzoic acid, and vitamin C in this plant. All the reported pharmacological activities of this plant could be due to the presence of these phytochemicals. This plant possesses strong antioxidant activity which includes total flavonoid content, total phenolic content, nitric oxide scavenging activity and free radical scavenging activity. This plant possesses multiple pharmacological activities including cognition‐enhancing, neuroprotective, antipsychotic, anti‐depressant, antibacterial, anti‐arthritic, anti‐malarial, analgesic, anti‐inflammatory, anti‐fertility, cardiovascular, locomotor, anxiolytic, wound healing activity, anti‐spasmodic, hypolipidemic, anti‐cancerous and iron‐chelating activity with different extracts of this plant as well as various phytoconstituents present in this plant. The objective of this review article is to discuss in detail the reported ethnopharmacological uses, phytochemistry and various pharmacological activities of C. paniculatus.

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“…ROS production was measured by uorogenic dye 2',7'-dichlorodihydro uorescein diacetate (DCFH 2DA ), which is oxidized by intracellular ROS, estimated according to Kumar et al (2015). The SH-SY5Y cells were seeded in 24-well plates (5 x 10 4 cells/ml) and exposed to Fb1 (150μM) following pretreatment with apocynin (100μM) and incubated for 24h.…”

Section: Estimation Of Intracellular Rosmentioning

confidence: 99%

“…Mitochondrial membrane potential (MMP) was determined uorometrically using cationic uorescent dye rhodamine 123, estimated according to Kumar et al (2015) which enters the mitochondrial matrix resulting in photo luminescent quenching which depends on MMP. The SH-SY5Y cells were seeded in 24well plates (5 x 10 4 cells/ml) and exposed to Fb1 (150μM) following pretreatment with apocynin (100μM) and incubated for 24h.…”

Section: Mitochondrial Membrane Potential (Mmp) Assaymentioning

confidence: 99%

Apocynin exerts neuroprotective effects in fumonisin b1 induced neurotoxicity via attenuation of oxidative stress and apoptosis

Krupashree

Rachitha

2022

Preprint

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The mycotoxin fumonisin b1 (Fb1) is produced by fusarium verticillioides, which commonly infects corn and other agricultural products. Fb1 found to be a source of vast range of toxic effects like hepatotoxicity, neurotoxicity and carcinogenicity in a number of animal species. However, the information available currently regarding neurotoxic effects exerted by Fb1 is scanty. Hence, the present study was aimed to evaluate the neurotoxic effects of Fb1 and the possible mechanisms of toxicity in mice and as well as the role of cytotoxic, oxidative stress and apoptosis in neuroblastoma (SH-SY5Y) cell line. In this context, the toxicity of Fb1 was examined in male albino mice. Apocynin with 25,50,100mg/kg b/wt was pretreated for 7 days oral administration. Fb1 6.75 mg/kg b/wt was injected subcutaneously for 5 days. A significant elevation of 5-HT was observed in mice treated with Fb1 in whole brain showed biogenic amines may reflect Fb1 neurotoxicity which was attenuated by pretreatment of apocynin. Fb1 treatment increased oxidative damage in the brain, as evidenced by a decrease in GSH level along with significant increases in ROS, lipid peroxidation, protein carbonyl. Apocynin supplementation significantly ameliorated these biological parameter changes. In addition, apocynin pretreatment normalized the degenerative changes in histology studies. The cytotoxicity of Fb1 was evaluated by MTT and LDH assay showed that, Fb1 induced dose-dependent cell death in SH-SY5Y cells and IC50 value was determined as 150µM. Fb1 showed elevated reactive oxygen species, depolarized mitochondrial membrane potential and TEM observation showed vacuolation in SH-SY5Y cells. Further, apoptotic changes revealed in DAPI staining and DNA damage in comet assay. To overcome these toxicological effects, apocynin was pretreated followed by Fb1 exposure for 24 h. Pretreatment with apocynin resulted in significant increase in cell viability, restored membrane integrity, reactive oxygen species level was maintained and neutralized apoptotic changes. The protein expression CAT, GPx downregulated with Fb1 treatment and apoptotic markers caspase-3 and caspase-8 was upregulated. Pretretment with apocynin restored the antioxidant enzymes and overexpressed apocynin markers also altered. Collectively, these results suggest that ROS is the main upstream signal leading to increased Fb1 mediated neurotoxicity in mice and SH-SY5Y cells. Use of an antioxidant apocynin reversed the toxin-induced oxidative stress and apoptosis by its antioxidant potency.

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Celastrus paniculatus Willd. mitigates t-BHP induced oxidative and apoptotic damage in C2C12 murine muscle cells

Cited by 14 publications

References 39 publications

Protective Effect of Celastrus paniculatus Seed Extract against Lead Acetate Induced Nephrotoxicity in Wistar Rats

Protective Effect of Celastrus paniculatus Seed Extract against Lead Acetate Induced Nephrotoxicity in Wistar Rats

An extensive review on phytochemistry and pharmacological activities of Indian medicinal plantCelastrus paniculatusWilld

Apocynin exerts neuroprotective effects in fumonisin b1 induced neurotoxicity via attenuation of oxidative stress and apoptosis

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