Celecoxib plays a multiple role to peripheral blood lymphocytes and allografts in acute rejection in rats after cardiac transplantation

Zhang, Xuefeng; Zhang, Fan; Liu, Hongyu; Sun, Guodong; Liu, Zonghong; Lü, Hang; Chi, Chao; Li, Chunyu

doi:10.1097/00029330-200901020-00015

Cited by 58 publications

(1 citation statement)

References 24 publications

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“…Then, the cells were washed twice in PBS, fixed in 0.5% paraformaldehyde, and subjected to flow cytometry to detect the percentage of CD4 + and CD8 + lymphocyte subpopulations as previously described. 6 …”

Section: Methodsmentioning

confidence: 99%

Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo

Wang¹,

Li²,

He³

et al. 2014

TCRM

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PurposeTo determine the effects of RNA interference (RNAi) on chondrocyte proliferation, function, and immunological rejection after allogenic tissue-engineered cartilage transplantation within bone matrix gelatin scaffolds.MethodsSeven million rat normal and RNAi chondrocytes were harvested and separately composited with fibrin glue to make the cell suspension, and then transplanted subcutaneously into the back of Sprague Dawley rats after being cultured for 10 days in vitro. Untransplanted animals served as the control group. The allograft and immunological response were examined at 1, 2, 4, 8, and 12 months postoperatively with hematoxylin and eosin histochemical staining, immunohistochemical staining (aggrecan, type II collagen, class I and II major histocompatibility complex), and flow cytometry for peripheral blood cluster of differentiation 4+ (CD4+) and CD8+ T-cells.ResultsThere was no infection or death in the rats except one, which died in the first week. Compared to the control group, the RNAi group had fewer eukomonocytes infiltrated, which were only distributed around the graft. The ratio of CD4+/CD8+ T-cells in the RNAi group was significantly lower than the normal one (P<0.05). There were many more positively stained chondrocytes and positively stained areas around the cells in the RNAi group, which were not found in the control group.ConclusionThe aggrecanase-1 and aggrecanase-2 RNAi for chondrocytes decreased the immunological rejection effect.

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Section: Methodsmentioning

confidence: 99%

Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo

Wang¹,

Li²,

He³

et al. 2014

TCRM

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Radiosynthesis, biodistribution and scintigraphy of the ^99mTc‐Teicoplanin complex in artificially infected animal models

Shah¹,

Khan²,

Khan³

2010

Labelled Comp Radiopharmac

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Radiocomplexation of Teicoplanin (TIN), a new glycopeptide antibiotic with technetium-99m, was investigated. The 99m Tc-TIN complex was assessed for its radiochemical permanence, in vitro stability in serum, binding with methicillin-resistant Staphylococcus aureus (MRSA), biodistribution in Model Rats (MRT) and for scintigraphic precision in Model Rabbit (MRB). Radiochemically, a stable 99m Tc-TIN was observed with 98.9070.50% yield and remained staunch more than 90% up to 120 min, by mixing TIN, 1.5 mg in 0.5 ml of saline with 2.5 mCi sodium pertechnetate and 150 ml of stannous chloride dihydrate at pH 5.4. The 99m Tc-TIN was found stable in serum with an insignificant undesirable yield of free and unhydrolyzed technetium (5.2570.10 and 13.570.14%, n = 10) up to 120 min of incubation. The 99m Tc-TIN showed in vitro binding with MRSA in the range of 55-65%. The 99m Tc-TIN showed almost six-fold elevated uptake in the infected (IFT) muscle of the MRT as compared with the inflamed (IFM) and normal (NL) muscles. This higher uptake of the 99m Tc-TIN in the IFT was scintigraphically confirmed after the whole body scanning of the MRB. The radiochemical eternalness with high yield, in vitro stability in serum, binding with MRSA, significant biodistribution performance, and scintigraphic precision posed the 99m Tc-TIN, a new glycopeptide radiotracer for the infection imaging.

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Fonctions métaboliques de l’apeline

Castan-Laurell

Dray

Knauf

et al. 2013

Physiologie Et Physiopathologie Du Tissu Adipeux

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Celecoxib plays a multiple role to peripheral blood lymphocytes and allografts in acute rejection in rats after cardiac transplantation

Cited by 58 publications

References 24 publications

Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo

Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo

Radiosynthesis, biodistribution and scintigraphy of the ^99mTc‐Teicoplanin complex in artificially infected animal models

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Celecoxib plays a multiple role to peripheral blood lymphocytes and allografts in acute rejection in rats after cardiac transplantation

Cited by 58 publications

References 24 publications

Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo

Experimental study of tissue-engineered cartilage allograft with RNAi chondrocytes in vivo

Radiosynthesis, biodistribution and scintigraphy of the 99mTc‐Teicoplanin complex in artificially infected animal models

Fonctions métaboliques de l’apeline

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Resources

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Radiosynthesis, biodistribution and scintigraphy of the ^99mTc‐Teicoplanin complex in artificially infected animal models