Cell activation by CpG ODN leads to improved electrofusion in hybridoma production

A method for inducing antigen-specific IgG production by in vitro immunization

“…The order of these steps appears to be important for obtaining good results. For the first antigen stimulation, CpG ODN (Bal et al, 2011;Kato et al, 2011) is applied with the antigen and effectively stimulates the B cells, especially the antibody-producing ones. In the expansion step, the T and B cells are stimulated directly.…”

Dielectrophoresis for Bioparticle Manipulation

“…Compared with the conventional sample preparation, DEP simplifies the enrichment steps and improves the efficiency of sample preparation. For cell fusion, the DEP technique can be used to improve the pairing precision of cells, which has made great contributions to crossbreeding [39], cancer immunotherapy [40], biomedical research [41,42] and other fields. Besides, the DEP separation technique has been used widely in the purification of vaccine and protein [43,44], cancer diagnosis [45] and cell sorting [46,47], which lays a solid foundation for the diagnosis and treatment of disease [48].…”

“…It has been widely applied in crossbreeding [39], cancer immunotherapy [40], biomedical research [41,42] and other fields. The most important step in cell fusion is accurate cell pairing, and the DEP technique has been used to improve the pairing precision of cells.…”

Inhibition of Gaussia luciferase Activity by a Monoclonal Antibody

“…Gluc was expressed and purified as described previously [21]. Briefly, expression vectors for Gluc were constructed using a pET-30a vector (Novagen, San Diego, CA).…”

“…We have developed a highly efficient electrofusion method that enables direct screening for antibodies with desired functions to establish a functional antibody against the enzyme [21]. We succeeded in establishing functional monoclonal antibodies against Gluc, which are capable of inhibiting its enzymatic activity.…”