Cell adhesion and response to synthetic nanopatterned environments by steering receptor clustering and spatial location

Cavalcanti‐Adam, Elisabetta Ada; Aydin, Daniel; Hirschfeld-Warneken, Vera C.; Spatz, Joachim P.

doi:10.2976/1.2976662

Cited by 112 publications

(98 citation statements)

References 85 publications

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“…Basement membranes are not the same in all tissues but differ in the geometry of 5-200 nm size pits, pores, protrusions, and fibers so that nanostructured biomaterials can mimic the spatial organization and separation distances which are characteristic of each specific basement membrane. 42 In the present study, rough and porous surfaces in which pillars and grooves are separated by a distance ,58 nm, which corresponds to the maximum distance required for correct establishment of focal adhesions, 25,43 were obtained from silicon and compared not only to flat silicon but also to plastic surfaces which are routinely utilized for traditional 2D cell cultures.…”

Section: Discussionmentioning

confidence: 99%

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

Zennaro¹,

Rastaldi²,

Bakeine³

et al. 2016

IJN

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Although it is well recognized that cell–matrix interactions are based on both molecular and geometrical characteristics, the relationship between specific cell types and the three-dimensional morphology of the surface to which they are attached is poorly understood. This is particularly true for glomerular podocytes – the gatekeepers of glomerular filtration – which completely enwrap the glomerular basement membrane with their primary and secondary ramifications. Nanotechnologies produce biocompatible materials which offer the possibility to build substrates which differ only by topology in order to mimic the spatial organization of diverse basement membranes. With this in mind, we produced and utilized rough and porous surfaces obtained from silicon to analyze the behavior of two diverse ramified cells: glomerular podocytes and a neuronal cell line used as a control. Proper differentiation and development of ramifications of both cell types was largely influenced by topographical characteristics. Confirming previous data, the neuronal cell line acquired features of maturation on rough nanosurfaces. In contrast, podocytes developed and matured preferentially on nanoporous surfaces provided with grooves, as shown by the organization of the actin cytoskeleton stress fibers and the proper development of vinculin-positive focal adhesions. On the basis of these findings, we suggest that in vitro studies regarding podocyte attachment to the glomerular basement membrane should take into account the geometrical properties of the surface on which the tests are conducted because physiological cellular activity depends on the three-dimensional microenvironment.

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Section: Discussionmentioning

confidence: 99%

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

Zennaro¹,

Rastaldi²,

Bakeine³

et al. 2016

IJN

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“…The mean speed decreases with time for all surfaces, most likely due to the decrease in the cell-free gap area. The ability of cells to migrate is a response that is coupled to cell adhesion, [65] where stronger cell adhesion coincides with a less migratory behavior displayed by the cells. Vinculin is necessary for binding cell surface integrin receptors to the ECM www.advancedsciencenews.com www.advhealthmat.de adhesion molecules, which in part controls the process of cell spreading and movement.…”

Section: Cell Migrationmentioning

confidence: 99%

“…[62][63][64] The feature sizes described in these studies are in the range of 80-300 nm and higher, which is over a density threshold corresponding to a spacing of integrin-adhesive RGD ligands per unit area, which is ≈70 nm. [13,65] Some common effects of ordered nanotopographies with feature sizes exceeding 70 nm on cells include (1) reduced area of adhesion complexes, (2) increased filopodia, (3) biased orientation, and (4) constant traction forces. By contrast, increased adhesion has been reported on random nanoposts.…”

Section: Cell Adhesionmentioning

confidence: 99%

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Zhukova

Hiepen

Knaus

et al. 2017

Adv Healthcare Materials

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Surface structuring of titanium-based implants is known to modulate the behavior of adherent cells, but the influence of different nanotopographies is poorly understood. The aim is to investigate preosteoblast proliferation, adhesion, morphology, and migration on surfaces with similar surface chemistry but distinct nanotopographical features. Sonochemical treatment and anodic oxidation are employed to fabricate disordered, mesoporous titania (TMS) and ordered titania nanotubular (TNT) topographies on titanium, respectively. Morphological evaluation reveals that cells are polygonal and well-spread on TMS, but display an elongated, fibroblast-like morphology on TNT surfaces, while they are much flatter on glass. Both nanostructured surfaces impair cell adhesion, but TMS is more favorable for cell growth due to its support of cell attachment and spreading in contrast to TNT. A quantitative wound healing assay in combination with live-cell imaging reveals that cell migration on TMS surfaces has a more collective character than on other surfaces, probably due to a closer proximity between neighboring migrating cells on TMS. The results indicate distinctly different cell adhesion and migration on ordered and disordered titania nanotopographies, providing important information that can be used in optimizing titanium-based scaffold design to foster bone tissue growth and repair while allowing for the encapsulation of drugs into porous titania layer

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“…Their results showed that cell activities, such as cell spreading, stress ber formation, and focal contacts were maximized on nanodot arrays with spacing of 28 and 58 nm, but were decreased on arrays with spacing of 73 and 85 nm. 8,41,42) These results indicated that cell adhesion sites should be located in optimal arrangements for enhancement of cell activities. Cell interactions with extracellular surfaces are mediated by clustering of integrins into focal adhesion complexes and activation of intracellular signaling cascades to the nucleus and cytoskeleton.…”

Section: Cell Morphologymentioning

confidence: 96%

Cell Activity on Type 316L Stainless Steel with Self-Organized Nanopores Formed by Anodic Polarization

et al. 2016

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In this study, we evaluated the osseoconductivity of Type 316L stainless steel with self-organized nanopores of three different average diameters (26, 90, and 177 nm), formed by anodic polarization. The proliferation, alkaline phosphatase activity, and morphology of MC3T3-E1 mouse osteoblast-like cells, cultured on the self-organized nanopores were evaluated. The cell densities on samples with the nanopores were higher than those on mechanically nished surfaces that were mirror-polished or ground with #2000 SiC paper. In particular, the highest cell density and alkaline phosphatase activity were obtained on the nanoporous sample with the smallest diameter of 26 nm. Cells on the samples with 26 nm nanopores extended further and spread more lopodia compared with cells on samples with the other surface morphologies. Therefore, we concluded that self-organized nanopores with an optimal diameter (e.g., 26 nm) on Type 316L stainless steel could enhance long-term cell activity.

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Cell adhesion and response to synthetic nanopatterned environments by steering receptor clustering and spatial location

Abstract: (2008) Cell adhesion and response to synthetic nanopatterned environments by steering receptor clustering and spatial location, HFSP Journal, 2:5, 276-285,

Cited by 112 publications

References 85 publications

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Cell Activity on Type 316L Stainless Steel with Self-Organized Nanopores Formed by Anodic Polarization

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