Cell adhesion on supported lipid bilayers functionalized with RGD peptides monitored by using a quartz crystal microbalance with dissipation

Zhu, Xiao-Hua; Wang, Zhao; Zhao, Ansha; Huang, Nan; Chen, Huadong; Zhou, Shuo; Xie, Xiaoguang

doi:10.1016/j.colsurfb.2014.01.032

Cited by 20 publications

(7 citation statements)

References 53 publications

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“…Further advances soon provided an ability to measure not only the mass adsorbed via changes in the frequency but also the viscoelastic properties of the adhered layer using the dissifactor D according to the Sauerbrey equation. 30,31 Thereaer, the ability to measure the mass and the viscoelastic properties of adsorbed materials in their native, hydrated states, quickly saw the technique applied to the study of biological systems, including protein and cellular interactions.…”

Section: Resultsmentioning

confidence: 99%

Facile immobilization of vascular endothelial growth factor on a tannic acid-functionalized plasma-polymerized allylamine coating rich in quinone groups

Xiong

Yang

et al. 2016

RSC Adv.

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Section: Resultsmentioning

confidence: 99%

Facile immobilization of vascular endothelial growth factor on a tannic acid-functionalized plasma-polymerized allylamine coating rich in quinone groups

Xiong

Yang

et al. 2016

RSC Adv.

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“…The technique enables in situ monitoring of adsorption without invasive labeling together with information about the viscoelastic properties of the adhered layer by recording the change in frequency and damping of an oscillating piezoelectric crystal . In biomaterial research, QCM‐D has previously been used to study protein adsorption, and also cell‐ and bacteria‐surface interactions . The adhesion and spreading behaviors of cells on various materials have been tested, such as titanium, gold, tantalum, chromium, polystyrene, PSox, silicon dioxide, supported lipid bilayers, and HA .…”

Section: Introductionmentioning

confidence: 99%

Using QCM-D to study the adhesion of human gingival fibroblasts on implant surfaces

Westas

Svanborg

Wallin

et al. 2015

J. Biomed. Mater. Res.

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Sealing the soft tissue-implant interface is one of the key issues in preventing transcutaneous implant-associated infections. A promising surface modification for improving osseointegration and possibly soft tissue integration is to coat the implant surface with hydroxyapatite (HA) nanoparticles. When new implant materials are developed, their ability to facilitate cell attachment and spreading are commonly investigated in vitro to establish their potential for good in vivo performance. However, commonly used techniques, such as microscopy methods, are time consuming, invasive, and subjective. This is the first study using quartz crystal microbalance with dissipation monitoring, where the real-time adhesion of biopsy-derived human gingival fibroblasts onto titanium and nanostructured HA was investigated. Experiments were performed for at least 16 h, and we found that cellular attachment and spreading kinetics can be followed in situ by observing the change in dissipation and frequency with time. Interestingly, a correlation between cell coverage and the magnitude of dissipation shift reached at the end of the experiment was found, but no such trend was observed for the frequency. Furthermore, the level of cell coverage was found to influence the cellular attachment and spreading behavior. No difference in cell response to the two surface types, Ti and nanostructured HA, was found.

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“…In the past several years, SLBs have gained attention as potential cell culture platforms. ,, However, it has been observed that SLBs generated from reconstituted lipids, as POPC, are not a favorable substrate for cell culture. Because phosphocholine lipids are zwitterionic, inert, and lack surface moieties essential for cell adhesion, cells are incapable of creating strong adhesions on these surfaces. ,,− To overcome this difficulty, several research groups have modified SLBs to enhance cell adherence with different approaches such as coating them with extracellular matrix (ECM) proteins, incorporating RGD moieties, and modifying with in situ peptides . However, all of these modified SLBs as cell culture platforms use a peripheral agent (antibody, peptide, ECM protein, etc.)…”

Section: Resultsmentioning

confidence: 99%

Supported Membrane Platform to Assess Surface Interactions between Extracellular Vesicles and Stromal Cells

Uribe

Liu

Mohamed

et al. 2020

ACS Biomater. Sci. Eng.

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Extracellular vesicles (EVs) are membrane-encapsulated particles secreted by eukaryotic cells that stimulate cell communication and horizontal cargo exchange. EV interactions with stromal cells can result in molecular changes in the recipient cell and, in some cases, lead to disease progression. However, mechanisms leading to these changes are poorly understood. A few model systems are available for studying the outcomes of surface interactions between EV membranes with stromal cells. Here, we created a hybrid supported bilayer incorporating EVs membrane material, called an extracellular vesicle supported bilayer, EVSB. Using EVSBs, we investigated the surface interactions between breast cancer EVs and adipose-derived stem cells (ADSCs) by culturing ADSCs on EVSBs and analyzing cell adhesion, spreading, viability, vascular endothelial growth factor (VEGF) secretion, and myofibroblast differentiation. Results show that cell viability, adhesion, spreading, and proangiogenic activity were enhanced, conditions that promote oncogenic activity, but cell differentiation was not. This model system could be used to develop therapeutic strategies to limit EV−ADSC interactions and proangiogenic conditions. Finally, this model system is not limited to the study of cancer but can be used to study surface interactions between EVs from any origin and any target cell to investigate EV mechanisms leading to cellular changes in other diseases.

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Cell adhesion on supported lipid bilayers functionalized with RGD peptides monitored by using a quartz crystal microbalance with dissipation

Cited by 20 publications

References 53 publications

Facile immobilization of vascular endothelial growth factor on a tannic acid-functionalized plasma-polymerized allylamine coating rich in quinone groups

Facile immobilization of vascular endothelial growth factor on a tannic acid-functionalized plasma-polymerized allylamine coating rich in quinone groups

Using QCM-D to study the adhesion of human gingival fibroblasts on implant surfaces

Supported Membrane Platform to Assess Surface Interactions between Extracellular Vesicles and Stromal Cells

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