Cell Adhesion to Biomaterials: Concept of Biocompatibility

Lotfi, Marzieh; Nejib, M.; Naceur, M.

doi:10.5772/53542

Cited by 57 publications

(71 citation statements)

References 54 publications

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“…7,8 Some cells prefer to attach to hydrophilic surfaces, while others have characteristic attraction towards hydrophobic ones. [20][21][22] Generally, hydrophobic surfaces are considered to be more protein-absorbent than hydrophilic ones, due to the strong hydrophobic interactions. 9,23 However, this could cause conformational changes or, sometimes, denaturation of proteins and, thus, lower the proliferation of cells on such surfaces.…”

Section: Introductionmentioning

confidence: 99%

Wettability studies of topologically distinct titanium surfaces

Kulkarni

Patil-Sen

Junkar

et al. 2015

Colloids and Surfaces B: Biointerfaces

121

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The contact area of the water droplet on hydrophobic titanium planar surface (foil) was found to decrease during evaporation, whereas the contact area of the droplet on hydrophobic nanorough titanium surfaces practically remained unaffected until the complete evaporation.This demonstrates that the surface morphology and roughness at the nanoscale level substantially affect the titanium dioxide surface-water droplet interaction, opposing to previous observations for microscale structured surfaces. The difference in surface topographic nanofeatures of nanostructured titanium surfaces could be correlated not only with the time-dependency of the contact area, but also with time-dependency of the contact angle and electrochemical properties of these surfaces.

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Section: Introductionmentioning

confidence: 99%

Wettability studies of topologically distinct titanium surfaces

Kulkarni

Patil-Sen

Junkar

et al. 2015

Colloids and Surfaces B: Biointerfaces

121

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“…In Figure , it is possible to note that aluminium foils are characterized by a much higher roughness compared with silicon. It is well known that the presence of surface irregularities enhances the cell adhesion to substrates . In contrast to Si samples, aluminium surfaces did not show any sign of RA adsorption.…”

Section: Resultsmentioning

confidence: 70%

“…Contrary to the silicon polished substrates, aluminium foils have a relatively rough surface, which promotes cell adhesion . On the other hand, this feature makes the visualization of the cell morphology more complicated in comparison with the smoother silicon surface.…”

Section: Resultsmentioning

confidence: 99%

Micro‐Raman detection of the differentiation state of SH‐SY5Y cells grown on silicon and aluminium substrates

Ricci

Sagini

Caponi

et al. 2018

J Raman Spectroscopy

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SH‐SY5Y cell line is a model for neural diseases originating from neuroblastoma, a paediatric embryonal malignancy that may differentiate and regress to a benign state. By monitoring the differentiation in single living cells, it could be possible to reach a better understanding of the process and, consequently, to develop new therapeutic approaches. In this work, we investigated the potential of Raman microspectroscopy to monitor biochemical changes in single living SH‐SY5Y cells treated with a combination of retinoic acid (RA) and brain‐derived neurotrophic factor to stimulate the differentiation toward a neuronal phenotype. The results show that the spectroscopic analysis is able to detect biochemical alterations consistent with the differentiation process. Moreover, we investigated also the influence of the cell culture substrate on the treatment outcome. Silicon and aluminium are 2 materials suitable for the spectroscopic analysis and of interest in neuroscience applications. Even though silicon has an ensured biocompatibility, it is subject to alterations after the treatment with RA. Aluminium revealed to be a cost‐effective cell culture substrate for the RA and brain‐derived neurotrophic factor treatment of SH‐SY5Y cells and particularly suitable for Raman microspectroscopy.

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“…24 It is closely related with the surface charge effect because most of mammalian cells and their microenvironment including adhesion proteins known to negatively charged, and inhibited to adhere on negatively charged surface. 35,36 ECM proteins such as fibronectin and collagen would mediate cell attachment and proliferation by providing favorable surface charge compared to uncoated surface. 37 Our result demonstrated that cancer cells were not effectively attach and spread on negatively charged uncoated gold electrode surface because enough electrostatic binding between cells and negatively charged surface was not created to generate detectable impedance value upon cell attachment.…”

Section: Surface Properties Effects On Cell Growthmentioning

confidence: 99%

Measurement of cell-substrate impedance and characterization of cancer cell growth kinetics with mathematical model

Park

Choi

Kim

2015

Int. J. Precis. Eng. Manuf.

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Non-invasive cell-substrate impedance sensing technique was used to monitor cancer cell attachment, spreading and proliferation as function of culture time. Impedance values were measured with cells cultured on collagen coated surface which exhibited moderate contact angle (54.5) and positively charge surface with the zeta potential (ζ) of +3.91 mV. Impedance was gradually increased and reached steady state after 2.6 hrs and 2.9 hrs with different impedance for SCC7 (2.7 Ω) and HeLa cells (1.1 Ω) respectively. Modified Gompertz mathematical growth model was used to determine growth kinetics, and showed maximum growth rate (µ m ) for SCC7 (7.9× 10 -4 hr -1 ) and HeLa cells (2×10 -5 hr -1 ) as well as both cells showed exponential increase of impedance following the lag period (λ) which required for cells to recover from the trypsinization and initiate attaching on electrode surface. The duration of lag period for SCC7 (0.7 hr) and HeLa cells (0.25 hrs ) demonstrated that HeLa cells exit lag period earlier than SCC7 cells and enter exponential growth which supports the rapid attachment and spread on substrate. Our result supported that the impedance measurement revealed cell-type specific growth characteristics and electrophysical properties that would be used to determine meaningful fingerprint for cancer cell study.

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Cell Adhesion to Biomaterials: Concept of Biocompatibility

Abstract: According to Oudet [7], polymers have different physical properties. The most important are their thermal conductivity reflecting polymers' behaviour under temperature changes.

Cited by 57 publications

References 54 publications

Wettability studies of topologically distinct titanium surfaces

Wettability studies of topologically distinct titanium surfaces

Micro‐Raman detection of the differentiation state of SH‐SY5Y cells grown on silicon and aluminium substrates

Measurement of cell-substrate impedance and characterization of cancer cell growth kinetics with mathematical model

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