Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics

Jia, Xu; Wu, Jian; Chen, Xiaohong; Hou, Simeng; Li, Yangyang; Zhao, Ling; Zhu, Yingting; Li, Zhidong; Deng, Caibin; Su, Wenru; Zhuo, Yehong

doi:10.1016/j.isci.2023.108024

Cited by 5 publications

(2 citation statements)

References 39 publications

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“…10, K and L ). Demographic information for the normal control and POAG macaques, along with the details of POAG characteristics in the macaques (e.g., deeper anterior chamber, increased cup-to-disc ratio, and thinning retinal nerve fiber layer), has been reported in the previous study ( 77 ). Regarding the distribution of DA receptors in the macaque retina, DRD1 and DRD2 were the main expressed subtypes, predominantly found in the interneurons ( Fig.…”

Section: Resultsmentioning

confidence: 97%

Modulating amacrine cell–derived dopamine signaling promotes optic nerve regeneration and preserves visual function

Zhang,

Xue,

Tang

et al. 2024

Sci. Adv.

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As part of the central nervous system, the optic nerve, composed of axons from retinal ganglion cells (RGCs), generally fails to regenerate on its own when injured in adult mammals. An innovative approach to promoting optic nerve regeneration involves manipulating the interactions between amacrine cells (ACs) and RGCs. Here, we identified a unique AC subtype, dopaminergic ACs (DACs), that responded early after optic nerve crush by down-regulating neuronal activity and reducing retinal dopamine (DA) release. Activating DACs or augmenting DA release with levodopa demonstrated neuroprotective effects and modestly enhanced axon regeneration. Within this context, we pinpointed the DA receptor D1 (DRD1) as a critical mediator of DAC-derived DA and showed that RGC-specific Drd1 overexpression effectively overcame subtype-specific barriers to regeneration. This strategy markedly boosted RGC survival and axon regeneration after crush and preserved vision in a glaucoma model. This study unveils the crucial role of DAC-derived DA signaling in optic nerve regeneration, holding promise for therapeutic insights into neural repair.

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Section: Resultsmentioning

confidence: 97%

Modulating amacrine cell–derived dopamine signaling promotes optic nerve regeneration and preserves visual function

Zhang,

Xue,

Tang

et al. 2024

Sci. Adv.

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“…Among these genes, ACTC1 had the highest node degree, which was 31. ATAC1 influences the contractile properties of smooth muscle [ 23 ]. Several nodes with high absolute values of log 2 FC were COMP, TNMD and PVALB.…”

Section: Resultsmentioning

confidence: 99%

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Li,

Wang,

Sun

et al. 2024

BMC Genomics

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Background Skeletal muscle is composed of muscle fibers with different physiological characteristics, which plays an important role in regulating skeletal muscle metabolism, movement and body homeostasis. The type of skeletal muscle fiber directly affects meat quality. However, the transcriptome and gene interactions between different types of muscle fibers are not well understood. Results In this paper, we selected 180-days-old Large White pigs and found that longissimus dorsi (LD) muscle was dominated by fast-fermenting myofibrils and soleus (SOL) muscle was dominated by slow-oxidizing myofibrils by frozen sections and related mRNA and protein assays. Here, we selected LD muscle and SOL muscle for transcriptomic sequencing, and identified 312 differentially expressed mRNA (DEmRs), 30 differentially expressed miRNA (DEmiRs), 183 differentially expressed lncRNA (DElRs), and 3417 differentially expressed circRNA (DEcRs). The ceRNA network included ssc-miR-378, ssc-miR-378b-3p, ssc-miR-24-3p, XR_308817, XR_308823, SMIM8, MAVS and FOS as multiple core nodes that play important roles in muscle development. Moreover, we found that different members of the miR-10 family expressed differently in oxidized and glycolytic muscle fibers, among which miR-10a-5p was highly expressed in glycolytic muscle fibers (LD) and could target MYBPH gene mRNA. Therefore, we speculate that miR-10a-5p may be involved in the transformation of muscle fiber types by targeting the MYHBP gene. In addition, PPI analysis of differentially expressed mRNA genes showed that ACTC1, ACTG2 and ACTN2 gene had the highest node degree, suggesting that this gene may play a key role in the regulatory network of muscle fiber type determination. Conclusions We can conclude that these genes play a key role in regulating muscle fiber type transformation. Our study provides transcriptomic profiles and ceRNA interaction networks for different muscle fiber types in pigs, providing reference for the transformation of pig muscle fiber types and the improvement of meat quality.

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Trabecular meshwork cell differentiation in response to collagen and TGFβ-2 spatial interactions

Lamont,

Wright,

Devries

et al. 2024

Acta Biomaterialia

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Cell atlas of trabecular meshwork in glaucomatous non-human primates and DEGs related to tissue contract based on single-cell transcriptomics

Cited by 5 publications

References 39 publications

Modulating amacrine cell–derived dopamine signaling promotes optic nerve regeneration and preserves visual function

Modulating amacrine cell–derived dopamine signaling promotes optic nerve regeneration and preserves visual function

Identification of different myofiber types in pigs muscles and construction of regulatory networks

Trabecular meshwork cell differentiation in response to collagen and TGFβ-2 spatial interactions

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