Cell block preparation in urine cytology: examination of utility and workflow in an academic practice

Dantey, Kossivi; Pantanowitz, Liron; Xing, Juan; Cuda, Jackie; Nestler, Rick

doi:10.1016/j.jasc.2018.11.001

Cited by 15 publications

(23 citation statements)

References 28 publications

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“…Xing et al suggested that clinical findings (eg, papillary tumor by imaging or cystoscopy) and/or cell block preparation, and increased numbers of monotonous single urothelial cells and 3‐dimensional cellular clusters without fibrovascular cores could be very helpful for identifying LGUN. The use of cell blocks (CBs) in urine cytology is utilized infrequently, and their diagnostic value has only been described in a limited number of studies, most of which were conducted on LUT specimens 15,64,65 …”

Section: Low‐grade Urothelial Neoplasia In Uut Cytologymentioning

confidence: 99%

“…The use of cell blocks (CBs) in urine cytology is utilized infrequently, and their diagnostic value has only been described in a limited number of studies, most of which were conducted on LUT specimens. 15,64,65 Simon et al 54 reported that evaluation of UUT cytology specimens both pre-and post-TPS showed higher sensitivity for the detection of HGUC versus…”

Section: Low-grade Urothelial Neoplasia In Uut Cytologymentioning

confidence: 99%

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A review of upper urinary tract cytology performance before and after the implementation of The Paris System

Zhang

Miki

Hang

et al. 2020

Cancer Cytopathology

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Urinary cytology (UC) is one of the primary diagnostic modalities used for the screening and surveillance of urothelial carcinoma. Despite its widespread use, UC has suffered from a lack of standardized or reproducible criteria and wide interobserver variability, particularly of the designation of atypical urothelial cells. The Paris System for Reporting Urinary Cytology (TPS), published in 2016, aimed to provide a standardized approach for evaluating UC by creating diagnostic categories with specific cytomorphologic criteria. Recent studies have primarily investigated the application of TPS on lower urinary tract specimens and have mostly shown that TPS implementation has improved the performance of UC specimens. Only a few studies have reported the impact of TPS on upper urinary tract (UUT) cytology. Additionally, there is uncertainty as to which cytological features are most predictive of high‐grade urothelial carcinoma (HGUC) in the UUT. This review summarizes the literature regarding the utility and performance of UUT cytology and highlights findings before and after the implementation of TPS.

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Section: Low‐grade Urothelial Neoplasia In Uut Cytologymentioning

confidence: 99%

Section: Low-grade Urothelial Neoplasia In Uut Cytologymentioning

confidence: 99%

A review of upper urinary tract cytology performance before and after the implementation of The Paris System

Zhang

Miki

Hang

et al. 2020

Cancer Cytopathology

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“…6 This study aims to determine whether urine cell block technique can improve the diagnostic sensitivity as compared to urine cytology alone for detecting urothelial carcinoma and also to evaluate the importance of p53 mutations and its diagnostic and prognostic value in bladder cancer Some of the previous international studies strongly support this view while according to some authors, cell block technique has no significant role in improving the diagnostic utility of urine cell cytology. 7,8 We didn't find any local study done regarding cell block technique in urine cytology. P53 expression is the most common gene involved in human cancers.…”

Section: Original Articlementioning

confidence: 99%

Utility of Cell Block with p53 Immunostain in Diagnosing Urothelial Carcinoma

Nabi¹,

Ali²,

Fatima³

et al. 2022

J Islamabad Med Dental Coll

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Background: Urine cytology is an initial noninvasive screening test done in patients with suspected urothelial carcinoma. The objective of this study was to compare the sensitivity of conventional smear cytology with cell block technique for the diagnosis of malignant cells in urine and to assess the efficacy of p53 immuno-marker in the diagnosis and prognosis of urinary bladder cancer. Methodology: The study was carried out in the department of Histopathology, Pakistan Institute of Medical Sciences(PIMS), Islamabad. Cell blocks and cytology smears and were prepared from urine samples of 80 patients of urothelial carcinoma and p53 stain was applied. Data was analyzed using version 21. Sensitivity of conventional smear and cell block techniques were calculated for both low and high grade urothelial carcinomas. Results:The sensitivity of detecting atypical cells using conventional smear was 31% and 70.6% for low grade and high grade urothelial tumors respectively. The sensitivity increased to 41.4% and 84.3% for low and high grade urothelial carcinomas respectively with cell block technique. Positive p53 stain was seen in 13.8% of cases with low grade tumors and 66.7% cases with high grade tumors. Chi-square test was used to find the association of cytology findings with the histologic grade. Conclusion: Sensitivity of urine cell blocks is higher than the conventional smear method for detecting high grade lesions. It can be used as an efficient non-invasive technique and an adjunct tool for the determination of disease relapse and overall survival in patients. Keywords: Carcinoma, Cytology, P53 Protein, Urine

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“…Morphological analysis of microscopic images has the advantages of traceability, visualization, meeting the gold standard of microscopy, drawing lessons from doctors' experience, and reporting particle openness, but it is an important technology that needs to improve detection methods and technical means [24], for example, effective detection methods, high-quality image acquisition methods, subsequent image processing, segmentation, pattern recognition, algorithm effectiveness, and accuracy.…”

Section: Morphological Analysis Of Microscopic Imagesmentioning

confidence: 99%

Aggregation of Nanochemical Microcrystals in Urine Promotes the Formation of Urinary Calculi

Lou

Song

2020

Journal of Chemistry

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With the increasing incidence and recurrence rate of urinary calculi, urinary calculi have become a serious health risk, and the research on urinary calculi has become the focus of public attention. At present, the research results on the formation mechanism of urinary calculi are not ideal, and there is no unified conclusion. In order to further study the influencing factors of the formation of urinary calculi and provide new ideas for the prevention and clinical treatment of urinary calculi, the influence of agglomeration of nanochemical microcrystals in urine on urinary calculi was studied in this paper. In this study, fresh morning urine was collected from 10 urological stone patients and 10 healthy controls without urological stone in the urology department of a hospital. After processing the experimental specimens, we first use flame atomic absorption spectrometry and alcian blue colorimetric method to detect the content of Ca2+ and citrate in the urine and then use the nanoparticle size analyzer to detect the microcrystals in the urine. Diameter, distribution, degree of aggregation and potential, and finally HRTEM observation to observe the morphology, chemical composition, and element composition of the nanocrystals. The results showed that the content of Ca2+ and lemon hydrochloric acid in the urine of the experimental group was lower than that of the control group. The particle size of the nanocrystals increased with the increase in the pore size of the membrane. The average particle size of the experimental group increased gradually from 163 ± 31 nm to 3219 ± 863 nm, while the average particle size of the control group increased from 183 ± 65 nm to 997 ± 522 nm. The mean value of the potential decreased with the increase in the pore size of the filter membrane. The change amplitude of the experimental group was 6.57 mV, while the change amplitude of the control group was only 1.75 mV. In the composition of nanocrystals, element O accounts for the most, accounting for 42.54% of all elements. This indicates that the aggregation of nanocrystals in urine will lead to the rapid increase in the size of nanocrystals, which will eventually lead to the formation of stones.

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Cell block preparation in urine cytology: examination of utility and workflow in an academic practice

Cited by 15 publications

References 28 publications

A review of upper urinary tract cytology performance before and after the implementation of The Paris System

A review of upper urinary tract cytology performance before and after the implementation of The Paris System

Utility of Cell Block with p53 Immunostain in Diagnosing Urothelial Carcinoma

Aggregation of Nanochemical Microcrystals in Urine Promotes the Formation of Urinary Calculi

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