2011
DOI: 10.1007/978-1-61779-080-5_7
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Cell Culture Contamination

Abstract: Microbial contamination is a major issue in cell culture, but there are a range of procedures which can be adopted to prevent or eliminate contamination. Contamination may arise from the operator and the laboratory environment, from other cells used in the laboratory, and from reagents. Some infections may present a risk to laboratory workers: containment and aseptic technique are the key defence against such risks. Remedial management of suspected infection may simply mean discarding a single potentially infe… Show more

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Cited by 41 publications
(23 citation statements)
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“…Scalp fibroblast cultures were more prone to fungal and bacterial contamination using the India ink test. This was identified by observation of an increase in turbidity, a change in color of the culture media, and by microscopic inspection [8].…”
Section: Resultsmentioning
confidence: 99%
“…Scalp fibroblast cultures were more prone to fungal and bacterial contamination using the India ink test. This was identified by observation of an increase in turbidity, a change in color of the culture media, and by microscopic inspection [8].…”
Section: Resultsmentioning
confidence: 99%
“…Direct culture is the most effective and a sensitive method for detecting Mycoplasma , but it is also the most difficult and time consuming (requiring up to 28 days). While DNA fluorochrome staining is an easy and relatively fast indirect procedures to perform (requiring up to 4 to 5 days), however it also has several limitations such as high cost, some equivocal staining results to interpret, and mandatory use of suitable positive and negative control slides (15). …”
Section: Discussionmentioning
confidence: 99%
“…For research purposes, mammalian cells are typically cultured in static culture and propagated by passaging at regular intervals, with supplemental medium changes as needed. This method is limited by the requirement for frequent manipulations, which results in variability of culture conditions and increased risk of contamination [3]ā€“[7]. Further, these culture methods are time intensive and require trained technicians to maintain large-scale cultures.…”
Section: Introductionmentioning
confidence: 99%