Cell culture surfaces with immobilized gold nanostars: a new approach for laser-induced plasmonic cell optoporation

Vanzha, Ekaterina; Pylaev, Timofey; Prilepskii, Artur Y.; Голубев, А. А.; Khlebtsov, Boris N.; Богатырев, В. А.; Khlebtsov, Nikolai G.

doi:10.1117/12.2269948

Cited by 5 publications

(7 citation statements)

References 12 publications

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“…Vanzha et al. ( 2017 ) proposed a new approach to solve this drawback. They immobilized gold nanostars on the culture plate and cells grown on it.…”

Section: Physical Methods Of Gene Deliverymentioning

confidence: 99%

Advanced physical techniques for gene delivery based on membrane perforation

et al. 2018

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Gene delivery as a promising and valid tool has been used for treating many serious diseases that conventional drug therapies cannot cure. Due to the advancement of physical technology and nanotechnology, advanced physical gene delivery methods such as electroporation, magnetoporation, sonoporation and optoporation have been extensively developed and are receiving increasing attention, which have the advantages of briefness and nontoxicity. This review introduces the technique detail of membrane perforation, with a brief discussion for future development, with special emphasis on nanoparticles mediated optoporation that have developed as an new alternative transfection technique in the last two decades. In particular, the advanced physical approaches development and new technology are highlighted, which intends to stimulate rapid advancement of perforation techniques, develop new delivery strategies and accelerate application of these techniques in clinic.

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“…Vanzha et al. ( 2017 ) proposed a new approach to solve this drawback. They immobilized gold nanostars on the culture plate and cells grown on it.…”

Section: Physical Methods Of Gene Deliverymentioning

confidence: 99%

Advanced physical techniques for gene delivery based on membrane perforation

et al. 2018

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“…In the case of plasmon resonant particles, their behaviour can be regulated by light excitation. The shape of some particles can serve as antennas for converting optical energy into heat [ 125 ]. Such a structure can allow the penetration of cell membranes in a controlled manner, which can be used for cell transfection.…”

Section: Biological Effect and Applicationmentioning

confidence: 99%

“…The use of Au NPs has been documented for diverse applications [ 153 ] in biological systems due to their unique physio-chemical properties, which can be easily tuned by tuning the chemical routes [ 125 ]. Au NPs possess the ability to undergo localized surface plasmon resonance (SPR), which depends on the particle’s size, shape, and surface morphology.…”

Section: Biological Effect and Applicationmentioning

confidence: 99%

Nanomaterial Shape Influence on Cell Behavior

Kladko

Falchevskaya

Serov

et al. 2021

IJMS

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Nanomaterials are proven to affect the biological activity of mammalian and microbial cells profoundly. Despite this fact, only surface chemistry, charge, and area are often linked to these phenomena. Moreover, most attention in this field is directed exclusively at nanomaterial cytotoxicity. At the same time, there is a large body of studies showing the influence of nanomaterials on cellular metabolism, proliferation, differentiation, reprogramming, gene transfer, and many other processes. Furthermore, it has been revealed that in all these cases, the shape of the nanomaterial plays a crucial role. In this paper, the mechanisms of nanomaterials shape control, approaches toward its synthesis, and the influence of nanomaterial shape on various biological activities of mammalian and microbial cells, such as proliferation, differentiation, and metabolism, as well as the prospects of this emerging field, are reviewed.

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“…Recently, we proposed a simple universal centrifuge (CF) based approach for AuNP assembling directly on the culture plastic . Highly efficient (about 97%) and safe optotransfection of HeLa cells with plasmid DNA was provided on the platforms of Au nanostars layers using an 808 nm continuous-wave (CW) laser. However, the main disadvantage of CW irradiation is bulk heating of the cell medium that has a sufficient negative effect both on cargoes and cells .…”

Section: Introductionmentioning

confidence: 99%

Optoporation and Recovery of Living Cells under Au Nanoparticle Layer-Mediated NIR-Laser Irradiation

Pylaev

Efremov

Avdeeva

et al. 2021

ACS Appl. Nano Mater.

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Laser optoporation systems are now increasingly used for intracellular delivery. However, data on the response of cells to radiation-induced nondamaging changes in the integrity of the membrane lipid bilayer remain limited. Traditionally, confocal laser scanning microscopy and electron microscopy are used for such studies, but they have limitations for in situ experiments. The modern capabilities of atomic force microscopy (AFM) combine the resolution of electron microscopy and the possibility of noninvasive lifetime imaging of cells in vitro. Herein we used long-term AFM mapping integrated with fluorescence microscopy imaging for investigation of the whole cell cycle from irradiation time point to the total recovery to the intact cell state. For the first time we performed a comprehensive study of long-term posteffects of continuous laser and pulsed laser on the mechanical properties and the membrane recovery of HeLa cells grown on the Au nanoparticle layers of various morphologies. The set of nonpenetrating agents with various sizes ranging from 1 to 1.5 nm for propidium iodide (PI) up to 6–8 nm for 40 kDa FITC-labeled dextran was used to control the delivery efficacy. The main parameters recorded with AFM scanning of cells are Young’s modulus (YM) and the cell surface topography. We revealed that self-healing of HeLa cell from the moment of irradiation to complete restoration of the membrane integrity is lasting 22–30 h when using a continuous-wave source and 2–5 h when using a pulsed laser, respectively. The estimated time elapse was in good correspondence with the relative change in YM during the entire experiment. Our findings demonstrate the capability of AFM coupled with fluorescent microscopy for further in situ investigations of the morphological and functional state of the cells exposed to the influence of other external conditions.

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Cell culture surfaces with immobilized gold nanostars: a new approach for laser-induced plasmonic cell optoporation

Cited by 5 publications

References 12 publications

Advanced physical techniques for gene delivery based on membrane perforation

Advanced physical techniques for gene delivery based on membrane perforation

Nanomaterial Shape Influence on Cell Behavior

Optoporation and Recovery of Living Cells under Au Nanoparticle Layer-Mediated NIR-Laser Irradiation

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