Cell-Culture System for Continuous Production of Toxoplasma gondii Tachyzoites

Evans, R.; Chatterton, J. M. W.; Ashburn, David A.; Joss, A. W. L.; Ho-Yen, D. O.

doi:10.1007/s100960050423

Cited by 35 publications

(30 citation statements)

References 17 publications

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“…Alternative in vitro methods, using MRC5 fibro-blasts complemented with FCS, were used for short studies assessing the effect of antimicrobial agents on T. gondii [3,10,21]. Additionally, continuous cell lines such as HeLa, LLC and Vero were also found to be suitable for the propagation of tachyzoites of T. gondii for the needs of a general hospital laboratory [19]. Recently, the good performances of the dye test [39] were reported with tachyzoites grown in cell cultures supplemented with FCS [1].…”

Section: Discussionmentioning

confidence: 99%

“…The batch of FCS, used in this study, may have contained factors which adversely affected tachyzoite growth. HeLa cells were previously reported [19] as being more effective than Vero cells for the production of T. gondii tachyzoites in FCS supplemented medium. Since the primary goal of this study was the establishment of a serum free medium for the production of N. caninum tachyzoites, Vero cells were selected to support tachyzoite proliferation [13,28,30].…”

Section: Discussionmentioning

confidence: 99%

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Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

et al. 2002

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-Neospora caninum and Toxoplasma gondii are cyst-forming coccidian parasites of human and veterinary clinical relevance. In vitro cultivation of the protozoans using Vero cells is usually performed in order to produce antigenic materials. Quantitative and qualitative comparisons of Vero cells grown in RPMI medium supplemented either with foetal calf serum (FCS), horse serum (HS) or a specific serum-free additive (DefCell) were performed. A serum-free cell culture system used to propagate N. caninum (NC-1 isolate) and T. gondii tachyzoites (Rh stain) were compared with the other two cell culture systems. FCS supplemented media was found to be more effective than the others in promoting Vero cells and N. caninum tachyzoites. However, it was found unable to support adequate T. gondii tachyzoite proliferation. Vero cells, T. gondii and N. caninum tachyzoite production gave similar growth patterns with either HS or DefCell supplemented media. Defcell was considered as a good alternative to supplement culture medium.Neospora caninum / Toxoplasma gondii / cell culture / serum-free medium Résumé -Utilisation d'un milieu sans sérum pour la production in vitro de tachyzoites de Neospora caninum et Toxoplasma gondii sur cellules Vero. Neospora caninum et Toxoplasma gondii sont deux sporozoaires présentant un intérêt en médecine humaine et vétérinaire. La culture in vitro utilisant, entre autres, les cellules Vero comme support de la multiplication des deux parasites, est généralement employée en vue de l'obtention de tachyzoïtes. Au cours de cette étude, une évaluation quantitative et 159 Vet. Res. 33 (2002) 159-168

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

et al. 2002

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“…Sin embargo, la producción de taquizoítos de T. gondii en sistemas de cultivo celular es favorable porque es de fácil manejo, bajo mantenimiento y no tiene tantas implicaciones éticas como el manejo de modelos animales (12)(13)(14).…”

Section: Discussionunclassified

Eficiencia de cultivo in vitro de Toxoplasma gondii en las líneas celulares THP1 y Vero

Cuéllar

Hernández

Villegas³

et al. 2012

biomedica

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planearon y realizaron los experimentos. Jorge Enrique Gómez participó en el diseño de los experimentos y en el análisis de los resultados. Todos los autores participaron en la redacción y aprobaron el texto del artículo. Introducción. El cultivo in vitro es un método importante para la obtención de Toxoplasma gondii con fines de diagnóstico clínico o biotecnológico. Objetivo. Determinar el porcentaje de invasión y producción de T. gondii en las líneas celulares THP1 y Vero. Materiales y métodos. Se determinó la curva de crecimiento para las células Vero y THP1 por conteo en hemocitómetro. Posteriormente, se identificó el porcentaje de invasión de T. gondii en células THP1 y Vero por citometría de flujo, en diferentes proporciones célula/taquizoíto de 1/5, 1/20, 1/50. Por otro lado, se calculó el índice de rendimiento de T. gondii, cepa RH, y del aislamiento CIBM1 en células THP1. Resultados. Las células Vero crecen más rápidamente que las células THP1, con un crecimiento exponencial en un periodo de siete días. El aislamiento CIBM1 infecta las células THP1 en las tres proporciones diferentes de 1/5,1/20 y 1/50 con porcentajes de invasión de 57,1 %, 15,5 % y 12,2 %, y en células Vero, de 25,3 %, 17,8 % y 8,8 %. La cepa RH de T. gondii mostró porcentajes de invasión más bajos, de 32,6 %, 14,8 % y 8,1 % en células THP1 y de 22,3 %, 14,1 % y 3,4 % en células Vero. Conclusiones. El aislamiento CIBM1 presentó mayor rendimiento con respecto a la cepa RH de T. gondii en células THP1, siendo estas células una buena línea para estudiar el proceso de invasión y probar candidatos farmacológicos para reducir la infección por T. gondii. Conclusions. The CIBM1 isolate had a higher yield than the RH strain of T. gondii in THP1 cells. THP1 cells were indicated to be a good model for the study of invasion and for the assays of new pharmacological candidates against T. gondii. NOTA TÉCNICA Eficiencia de cultivo in vitro de

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“…11 They fo und the op ti mal mul tip li city of in fec ti on was re ac hed at 1:1 tach yzo i te to cell ra ti o. We al so used this ra ti o du ring our study.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Cultivation of Toxoplasma Gondii in Various Cell Cultures

Akarsu¹,

Biriken²

2010

Turkiye Klinikleri J Med Sci

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A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: :The pur po se of this study was to eva lu a te va ri o us cell cul tu res such as Afri can gre en mon key kid ney cells (Ve ro), hu man cer vix ade no car si no ma cells (He La) and pri mary fe tal bo vi ne kid ney cells (FBK), which we cul tu red and sub cul tu red in our la bo ra tory for To xoplas ma growth ra te and yi eld. M Ma a t te e r ri i a al l a an nd d M Me et t h ho od ds s: : To xop las ma gon di i tach yzo i tes we re ob ta i ned by was hing pe ri to ne al ca vi ti es of al bi no mi ce which we re ino cu la ted in tra pe ri to ne ally thre e days pri or to cell cul tu re in fec ti on. The tach yzo i tes in the pe ri to ne al exu da tes we re pu ri fi ed, po o led and used to in fect thre e types of cells: Ve ro, He La and pri mary fe tal bo vi ne kid ney cells. Tach yzo i te multip li ca ti on ra te and yi eld of the se thre e cells we re eva lu a ted mic ros co pi cally both in the cell cul ture flasks and from the har vests. R Re e s su ul lt ts s: : All of the cell types ga ve rep ro du cib le re sults, with over 1x10 6 tach yzo i tes in the har vests, mo re than 90% of which we re vi ab le. Tach yzo i te yi eld from Vero cells was sig ni fi cantly hig her than He La cells. Ve ro and FBK cells we re in fec ted with tach yzo ites fas ter than He La cell cul tu re and the har vest ti mes we re mo re stab le. C Co on nc c l lu u s si i o on n: : To xop las ma gon di i tach yzo i te growth was hig her in Ve ro cells than in He La cells and it is a con ti nu o us cell line, dif fe ring from pri mary FBK cells. The re fo re, Ve ro cell li ne was pre fe rab le among thre e cell types as a tach yzo i te so ur ce for con se cu ti ve di ag nos tic and in ves ti ve use.K Ke ey y W Wo or rd ds s: : Toxoplasma; cell culture techniques Ö ÖZ ZE ET T A Am ma aç ç: : Bu ça lış ma da, la bo ra tu va rı mız da pri mer kül tür ve pa saj la rı nı yap tı ğı mız Af ri ka ye şil may mun böb rek hüc re le ri (Ve ro), in san ser viks ade no kar si nom hüc re le ri (He La) ve pri mer fe tal da na böb rek hüc re le ri (FBK) gi bi çe şit li hüc re kül tür le ri ni, To xop las ma gon di i' nin üre me hı zı ve verim li lik le ri açı sın dan de ğer len dir me yi amaç la dık. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : To xop las ma gon di i ta ki zoit le ri, hüc re kül tür le ri ne ekil me sin den üç gün ön ce in tra pe ri to ne al ola rak ino kü le edi len al bi no fa re ler den, pe ri ton boş lu ğu nun yı kan ma sı ile el de edil miş tir. Pe ri ton ek sü da la rın da ki ta ki zo it ler ay rış tı rıl mış, bir leş ti ril miş ve Ve ro, He La ve FBK hüc re kül tür le ri ne ekim için kul la nıl mış tır. Hüc -re kül tür le rin de ki ta ki zo it ço ğal ma hı zı ve mik ta rı, hem hüc re kül tür ta ba ka la rı nın hem de top lanan be si yer le ri nin mik ros ko pik ola rak de ğer len di ril me siy le be lir len miş tir. B Bu ul l g gu u l la ar r: : Tüm hüc re tip le ri, 1x10 6 'nın üze rin de ve %90'ın dan faz la sı can lı olan ta ki zo it üre me so nuç la rı ...

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Cell-Culture System for Continuous Production of Toxoplasma gondii Tachyzoites

Cited by 35 publications

References 17 publications

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

Use of a serum-free medium to produce in vitro Neospora caninum and Toxoplasma gondii tachyzoites on Vero cells

Eficiencia de cultivo in vitro de Toxoplasma gondii en las líneas celulares THP1 y Vero

In Vitro Cultivation of Toxoplasma Gondii in Various Cell Cultures

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