Cell Cycle Activation During Imbibition and Visible Germination in Embryos and Megagametophytes of Jack Pine (Pinus banksianaLamb.)

Wyman, Janet; Tremblay, M. Francine; Laliberté, Sylvie

doi:10.1006/anbo.1996.0118

Cited by 6 publications

(2 citation statements)

References 29 publications

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“…Thus, DNA replication precedes pepper seedling growth. An increase in 4C nuclei also marks an advancement in germination in jack pine (Pinus banksiana), sugarbeet, coffee (Coffea arabica) and barley (Hordeum vulgare) (Wyman et al, 1996;da Silva et al, 2008;Gendreau et al, 2008). The 4C/2C ratio remains relatively constant (about 0.1) until 12 h, slightly increasing up to 60 h and then increasing fourfold to exceed 1 at 72 h of germination.…”

Section: Dna Replication During Germinationmentioning

confidence: 99%

“…The 4C/2C ratio remains relatively constant (about 0.1) until 12 h, slightly increasing up to 60 h and then increasing fourfold to exceed 1 at 72 h of germination. An increase in 4C nuclei also marks an advancement in germination in jack pine ( Pinus banksiana ), sugarbeet, coffee ( Coffea arabica ) and barley ( Hordeum vulgare ) (Wyman et al , 1996; Sliwinska et al , 1999; da Silva et al , 2008; Gendreau et al , 2008). In contrast, in the nuclei of the radicle and young seedlings (up to an axis length of 2 mm) of barrel clover ( Medicago truncatula ) there is no nuclear DNA replication but the proportion of 4C nuclei is as high as 45% (Faria et al , 2005).…”

Section: Dna Replication During Germinationmentioning

confidence: 99%

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Nuclear DNA replication and seed quality

Śliwińska

2009

Seed Sci. Res.

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The quality of a seed (germination and vigour) is established during its development and maturation, but can be improved by post-harvest processing and pre-sowing treatments. During commercial seed production, maturity is usually estimated visually, relying on experience of the growers, but seed researchers are working to find molecular markers that can be applied easily to help in establishing optimal harvest time. One marker is cell cycle activity expressed as DNA replication in the seeds, analysed by flow cytometry. This fast and accurate method for the estimation of DNA content in plant nuclei allows detection of nuclei at different replication stages in different seed tissues and thus makes it possible to follow changes in the physiological state of a seed. DNA replication, as a late event during germination, can also be used to mark completion of germination and transition to early seedling growth. This information can be useful in the evaluation of seed quality and for following the advancement of priming. Flow cytometric analysis of ploidy can be also used as a basis for control of purity of some polyploid species seed lots.

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Section: Dna Replication During Germinationmentioning

confidence: 99%