Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction From Toddalia asiatica (L.) Lam.

Li, Xun; Qiu, Zi-Dong; Jin, Qinghao; Chen, Guilin; Chen, Guofang

doi:10.3389/fphar.2018.00629

Cited by 24 publications

(15 citation statements)

References 49 publications

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“…When cells undergo apoptotic change due to cell membrane damage, PS translocate from the inner leaflet of the plasma membrane to the outer leaflet, which is considered as an early event in apoptosis. 27 In the present study, we found that S. cumini-induced apoptosis in OSCC cells through the induction of phosphatidylserine externalization and these morphological changes related to apoptosis further substantiate our dual staining investigation.…”

Section: Discussionsupporting

confidence: 87%

Syzygium cumini extract induced reactive oxygen species‐mediated apoptosis in human oral squamous carcinoma cells

Ezhilarasan

Apoorva

Vardhan

2018

J Oral Pathology Medicine

342

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Background Syzygium cumini (L.) Skeels (jambolan) is commonly used in Indian traditional medicine to treat a variety of diseases such as obesity, diabetes etc. The cytotoxic potential of S. cumini (SC) against oral cancer cell line remains elusive. Therefore, in this study, we evaluated the cytotoxic effect of S. cumini in human oral squamous cell carcinoma (OSCC) cell line (SCC‐25 cells). Material and Methods Oral squamous cell carcinoma cells are treated with different concentrations (10, 20, and 40 μg/mL) of S. cumuni for 24 hours and cytotoxicity was analyzed by MTT assay. The intracellular reactive oxygen species (ROS) was measured using the indicator dye, 2′,7′‐dichlorofluorescin diacetate staining. Apoptosis‐related morphological changes were evaluated by dual acridine orange/ethidium bromide (AO/EB) fluorescent staining and phosphatidylserine externalization was measured by annexin V assays. The protein and gene expression of cadherin‐1 was evaluated by western blotting and PCR analysis. Results Syzygium cumini treatments caused cytotoxicity of OSCC cell line and induced intracellular ROS accumulation. This treatment also caused apoptosis‐related morphological changes and externalization of phosphatidylserine in OSCC cells. Further, S. cumini treatments increased protein and gene expression of cadherin‐1. Conclusion Syzygium cumini extract inhibits the proliferation of OSCC cells and induces apoptosis through ROS accumulation and therefore, it could be used for the prevention of OSCC.

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Section: Discussionsupporting

confidence: 87%

Syzygium cumini extract induced reactive oxygen species‐mediated apoptosis in human oral squamous carcinoma cells

Ezhilarasan

Apoorva

Vardhan

2018

J Oral Pathology Medicine

342

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“…Treating HT-29 human colon cancer cell line with this plant product promoted both intrinsic and extrinsic pathways of apoptosis by enhancing the activity of caspases -8, -9, and -3. Moreover, ROS generation appears to be the main reason for the cell cycle arrest in these cells following extract exposure [ 66 ]. Ethanolic extract of the rhizome of Cyperus rotundus (known as coco-grass, Java grass, nutgrass, purple nutsedge or purple nutsedge, red nut sedge, Khmer kravanh chruk) suppresses the proliferation of triple-negative human breast cancer cell line, MDA-MB-231.…”

Section: Major Apoptotic Pathwaysmentioning

confidence: 99%

The Most Competent Plant-Derived Natural Products for Targeting Apoptosis in Cancer Therapy

et al. 2021

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Cancer is a challenging problem for the global health community, and its increasing burden necessitates seeking novel and alternative therapies. Most cancers share six basic characteristics known as “cancer hallmarks”, including uncontrolled proliferation, refractoriness to proliferation blockers, escaping apoptosis, unlimited proliferation, enhanced angiogenesis, and metastatic spread. Apoptosis, as one of the best-known programmed cell death processes, is generally promoted through two signaling pathways, including the intrinsic and extrinsic cascades. These pathways comprise several components that their alterations can render an apoptosis-resistance phenotype to the cell. Therefore, targeting more than one molecule in apoptotic pathways can be a novel and efficient approach for both identifying new anticancer therapeutics and preventing resistance to therapy. The main purpose of this review is to summarize data showing that various plant extracts and plant-derived molecules can activate both intrinsic and extrinsic apoptosis pathways in human cancer cells, making them attractive candidates in cancer treatment.

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“…T. asiatica has played a very important role in traditional medicine, and the whole plant can be used as medicine, especially its root often used in the folk ( Yang et al, 2013 , Li et al, 2018 ). The studies of chemical composition showed that the main chemical components were alkaloids and coumarins in T. asiatica ( Wang et al, 2009 , Hu et al, 2014 , Sukieum et al, 2018 , Lin and Chen, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

A systematic review on traditional medicine Toddalia asiatica (L.) Lam.: Chemistry and medicinal potential

Zeng

Tian

Feng

et al. 2021

Saudi Pharmaceutical Journal

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Toddalia asiatica (L.) Lam., belonging to Toddalia genus of Rutaceae family, is a folk medicine in China used for hundreds of years. The whole plant can be used as medicine, especially the root that used to be applied in the folk. In recent decades, with the in-depth research from domestic and foreign researchers, it has gradually been discovered that the chemical components in T. asiatica are mainly coumarins and alkaloids. Its pharmacological effects are manifested in anti-inflammatory and analgesic, hemostatic coagulation, anti-tumor, treatment of cardiovascular diseases, etc. It has a wide range of clinical applications and significant effects on rheumatism, pain, wound bleeding, and bruises. Due to its important research value, in this article, the chemical compositions and pharmacological effects of T. asiatica are comprehensively expounded in recent years in order to provide a reference for the related research and application of this medicinal material, which were carried out through a bibliometric search using the Science Citation Index- Expanded (SCIE) database, web of science, Google scholar and Chinese National Knowledge Infrastructure (CNKI) and all that.

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Cell Cycle Arrest and Apoptosis in HT-29 Cells Induced by Dichloromethane Fraction From Toddalia asiatica (L.) Lam.

Cited by 24 publications

References 49 publications

Syzygium cumini extract induced reactive oxygen species‐mediated apoptosis in human oral squamous carcinoma cells

Syzygium cumini extract induced reactive oxygen species‐mediated apoptosis in human oral squamous carcinoma cells

The Most Competent Plant-Derived Natural Products for Targeting Apoptosis in Cancer Therapy

A systematic review on traditional medicine Toddalia asiatica (L.) Lam.: Chemistry and medicinal potential

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