Cell cycle arrest induced by MPPa-PDT in MDA-MB-231 cells

Abstract

Photodynamic therapy (PDT) is a medical treatment using a photosensitizing agent and light source to treat cancers. Pyropheophorbidea methyl ester (MPPa), a derivative of chlorophyll, is a novel potent photosensitizer. To learn more about this photosensitizer, we examined the cell cycle arrest in MDA-MB-231. Cell cycle and apoptosis were measured by flow cytometer. Checkpoints of the cell cycle were measured by western blot. In this study, we found that the expression of Cyclin D1 was obviously decreased, while the expression of Chk2 and P21 was increased after PDT treatment. This study showed that MPPa-PDT affected the checkpoints of the cell cycle and led the cells to apoptosis.

Photodynamic therapy induced cell cycle arrest and cancer cell synchronization: review

“…Liang and co-workers showed that pyropheophorbide-a methyl ester (MPPa), a derivative of chlorophyll, has PS effects in PDT on MDA-MB-231 cells and induces cell death via apoptosis and CCA at S phase. Additionally, they verified that following PDT therapy, Chk2 and P21 expression increased, whereas cyclin D1 expression was dramatically reduced (145). Recently, nanoparticles, as carrier to deliver PS drugs, have attracted researchers in this area.…”

“…illustrated that curcumin in metal and encapsulation complex enhanced PDT efficiency. They found that encapsulated curcumin at concentration of 15 μM can arrest CC at G2/M phase in A549 cells ( 141 , 145 ). In a study conducted by Lange et al., cell death pathways were compared under the porphyrin derivative 5,10,15,20-tetra( m -hydroxyphenyl) chlorin (mTHPC, temoporfin), as named Foscan in Europe (trade name) on various cell lines (A-427, BHY, KYSE-70, RT-4, and SISO) of head and neck cancers.…”