Cell cycle arrest mediated by WEE1 is involved in the unfolded protein response in plants

Ko, Ki Seong; Yoo, Ji Myong; Ramasamy, Nirmal Kumar; Harmoko, Rikno; Vu, Bich Ngoc; Park, Ji Ye; Lee, Kyun Oh

doi:10.1007/s11816-018-0496-5

Cited by 3 publications

(3 citation statements)

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“…Therefore, WEE1 in plants does possess molecular features to target other proteins and residues that should be considered for the understanding of its role. Hence, unlike in human, WEE1 could be involved in more plant developmental processes and especially in various stress responses 14 – 16 , 26 , 27 . Previous studies concurred in showing a conservation of the expression profiles of WEE1 for plants subjected to a variety of stresses or under development processes including taxonomically distant species like Solanum lycopersicum 5 , Zea mays 3 , A. thaliana 4 , 6 , M. truncatula 14 , 15 and Brachypodium dystachion 16 .…”

Section: Introductionmentioning

confidence: 99%

Structural conservation of WEE1 and its role in cell cycle regulation in plants

Détain

Redecker

Leborgne-Castel

et al. 2021

Sci Rep

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The WEE1 kinase is ubiquitous in plant development and negatively regulates the cell cycle through phosphorylations. However, analogies with the control of the human cell cycle by tyrosine- (Tyr-) phosphorylation of cyclin-dependent kinases (CDKs) are sometimes questioned. In this in silico study, we assessed the structural conservation of the WEE1 protein in the plant kingdom with a particular focus on agronomically valuable plants, the legume crops. We analyzed the phylogenetic distribution of amino-acid sequences among a large number of plants by Bayesian analysis that highlighted the general conservation of WEE1 proteins. A detailed sequence analysis confirmed the catalytic potential of WEE1 proteins in plants. However, some substitutions of an arginine and a glutamate at the entrance of the catalytic pocket, illustrated by 3D structure predictions, challenged the specificity of this protein toward the substrate and Tyr-phosphorylation compared to the human WEE1. The structural differences, which could be responsible for the loss of specificity between human and plants, are highlighted and suggest the involvement of plant WEE1 in more cell regulation processes.

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Section: Introductionmentioning

confidence: 99%

Structural conservation of WEE1 and its role in cell cycle regulation in plants

Détain

Redecker

Leborgne-Castel

et al. 2021

Sci Rep

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“…ROS are important signaling molecules involved in many cellular processes, including stress responses. Normally, a balance is maintained between ROS generation and their removal by antioxidant defense systems, but this balance can be disrupted under stressful conditions, leading to ROS accumulation, which, if uncontrolled, can result in oxidative stress and cell death [51,52]. The impact of HY5 mutations on ROS levels in Chinese cabbage under TM-induced ER stress was examined.…”

Section: Mutations In the Hy5 Gene Reduce Ros Generation In Chinese C...mentioning

confidence: 99%

“…The nitroblue tetrazolium (NBT, Sigma-Aldrich Co., St. Louis, USA) and 3,3 -diam inobenzidine (DAB, Sigma-Aldrich Co., St. Louis, USA) staining methods were adapted for in situ detection of O 2 − and H 2 O 2 , respectively [51,58,59]. Chinese cabbage seedlings were grown on normal growth media and on media treated with 150 ng/mL TM for 7 d. Untreated seedlings, grown under identical conditions, served as experimental controls.…”

Section: Nitroblue Tetrazolium (Nbt) and 33 -Diaminobenzidine (Dab) S...mentioning

confidence: 99%

CRISPR/Cas9-Mediated HY5 Gene Editing Reduces Growth Inhibition in Chinese Cabbage (Brassica rapa) under ER Stress

Lee,

Ko,

Lee

et al. 2023

IJMS

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Various stresses can affect the quality and yield of crops, including vegetables. In this study, CRISPR/Cas9 technology was employed to examine the role of the ELONGATED HYPOCOTYL 5 (HY5) gene in influencing the growth of Chinese cabbage (Brassica rapa). Single guide RNAs (sgRNAs) were designed to target the HY5 gene, and deep-sequencing analysis confirmed the induction of mutations in the bZIP domain of the gene. To investigate the response of Chinese cabbage to endoplasmic reticulum (ER) stress, plants were treated with tunicamycin (TM). Both wild-type and hy5 mutant plants showed increased growth inhibition with increasing TM concentration. However, the hy5 mutant plants displayed less severe growth inhibition compared to the wild type. Using nitroblue tetrazolium (NBT) and 3,3′-diaminobenzidine (DAB) staining methods, we determined the amount of reactive oxygen species (ROS) produced under ER stress conditions, and found that the hy5 mutant plants generated lower levels of ROS compared to the wild type. Under ER stress conditions, the hy5 mutant plants exhibited lower expression levels of UPR- and cell death-related genes than the wild type. These results indicate that CRISPR/Cas9-mediated editing of the HY5 gene can mitigate growth inhibition in Chinese cabbage under stresses, improving the quality and yield of crops.

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