Cell cycle–dependent phosphorylation of Sec4p controls membrane deposition during cytokinesis

Lepore, Dante; Spassibojko, Olya; Pinto, Gabrielle; Collins, Ruth

doi:10.1083/jcb.201602038

Cited by 14 publications

(13 citation statements)

References 78 publications

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“…Correspondingly, the Ypt1 phosphomutant retained its localization to the Golgi yet was unable to provide the essential function of Ypt1. A previous study demonstrated that phosphorylation of another Rab GTPase, Sec4, is a negative regulatory mechanism coupled to the cell cycle 55 .…”

Section: D Phosphoproteome Analysis Provides Insights Into Function mentioning

confidence: 99%

In-depth and 3-Dimensional Exploration of the Budding Yeast Phosphoproteome

Lanz

Yugandhar

Gupta

et al. 2019

Preprint

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Phosphorylation is one of the most dynamic and widespread post-translational modifications regulating virtually every aspect of eukaryotic cell biology. Here we present a comprehensive phosphoproteomic dataset for budding yeast, comprised of over 30,000 high confidence phosphorylation sites identified by mass spectrometry. This single dataset nearly doubles the size of the known phosphoproteome in budding yeast and defines a set of cell cycle-regulated phosphorylation events. With the goal of enhancing the identification of functional phosphorylation events, we performed computational positioning of phosphorylation sites on available 3D protein structures and systematically identified events predicted to regulate protein complex architecture. Results reveal a large number of phosphorylation sites mapping to or near protein interaction interfaces, many of which result in steric or electrostatic "clashes" predicted to disrupt the interaction. Phosphorylation site mutants experimentally validate our predictions and support a role for phosphorylation in negatively regulating protein-protein interactions. With the advancement of Cryo-EM and the increasing number of available structures, our approach should help drive the functional and spatial exploration of the phosphoproteome.

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Section: D Phosphoproteome Analysis Provides Insights Into Function mentioning

confidence: 99%

In-depth and 3-Dimensional Exploration of the Budding Yeast Phosphoproteome

Lanz

Yugandhar

Gupta

et al. 2019

Preprint

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“…These results raise the possibility that abscission inhibition in cells with chromatin bridges requires modulation of exocyst-dependent, membrane-remodeling events at the abscission site. To gain insight into cytokinesis membrane trafficking events in cells with chromatin bridges, we followed the kinetics of the vesicle marker Sec4, which accumulates in the bud neck at the end of mitosis and controls membrane trafficking during cytokinesis ( Figure 8B ; Lepore et al. , 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Distinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeast

Masgrau

Battola

Sanmartín

et al. 2017

MBoC

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“…Furthermore, the polo-like kinase triggers activation of both the FEAR and MEN pathways during anaphase, promoting Cdc14 release and activation [10]. Finally, Cdc5 kinase activity is also important to cytokinesis [161][162][163][164].…”

Section: Interplay Between the Mitotic Checkpoints: A Central Role Fomentioning

confidence: 99%

Regulation of Mitotic Exit by Cell Cycle Checkpoints: Lessons From Saccharomyces cerevisiae

Matellán

Monje-Casas

2020

Genes

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In order to preserve genome integrity and their ploidy, cells must ensure that the duplicated genome has been faithfully replicated and evenly distributed before they complete their division by mitosis. To this end, cells have developed highly elaborated checkpoints that halt mitotic progression when problems in DNA integrity or chromosome segregation arise, providing them with time to fix these issues before advancing further into the cell cycle. Remarkably, exit from mitosis constitutes a key cell cycle transition that is targeted by the main mitotic checkpoints, despite these surveillance mechanisms being activated by specific intracellular signals and acting at different stages of cell division. Focusing primarily on research carried out using Saccharomyces cerevisiae as a model organism, the aim of this review is to provide a general overview of the molecular mechanisms by which the major cell cycle checkpoints control mitotic exit and to highlight the importance of the proper regulation of this process for the maintenance of genome stability during the distribution of the duplicated chromosomes between the dividing cells.

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Cell cycle–dependent phosphorylation of Sec4p controls membrane deposition during cytokinesis

Abstract: The GTPase Sec4p is a critical regulator of polarized membrane traffic. Lepore et al. show that the polo-like kinase Cdc5p phosphorylates Sec4p, which promotes coordinated membrane deposition during cytokinesis.

Cited by 14 publications

References 78 publications

In-depth and 3-Dimensional Exploration of the Budding Yeast Phosphoproteome

In-depth and 3-Dimensional Exploration of the Budding Yeast Phosphoproteome

Distinct roles of the polarity factors Boi1 and Boi2 in the control of exocytosis and abscission in budding yeast

Regulation of Mitotic Exit by Cell Cycle Checkpoints: Lessons From Saccharomyces cerevisiae

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