Cell death in HeLa cells upon imperatorin and cisplatin treatment

Abstract

Abstract:There is growing evidence that commonly applied chemotherapy regimens can be improved by introducing new, specific, active and low side-effect drugs, or by combining substances to obtain the required clinical effect. The aim of the present study was to investigate the effects of imperatorin and cisplatin, applied separately or in combination, on apoptosis, necrosis and autophagy induction in the human cervical carcinoma cell line (HeLa). Imperatorin appeared to be a potent autophagy inducer, rather than a necrotic or apoptotic one. In contrast, cisplatin induced mainly apoptosis and necrosis after 6 h and 24 h, while longer incubation resulted only in necrosis induction. When HeLa cells were incubated with both drugs, autophagy appeared most frequently, although to a smaller extent than that observed after imperatorin administered alone. At the molecular level, autophagy was correlated with the presence of the cleaved form of microtubule-associated protein 1 light chain LC3 -LC3II. It was also accompanied by the inhibition of heat shock proteins Hsp27 and Hsp72 expression. Our results indicate that imperatorin alone, or in combination with cisplatin, is mainly an autopahgy

Quantitative Analysis of Isopimpinellin from Ammi majus L. Fruits and Evaluation of Its Biological Effect on Selected Human Tumor Cells

“…Therefore, it is extremely important to search for new bioactive substances of plant origin that can increase the effectiveness and reduce the systemic toxicity observed during anticancer treatment. Therefore, in cancer therapy, attention is paid to natural substances [ 45 , 46 , 47 , 48 , 49 , 50 ], and numerous studies have confirmed the effectiveness of furanocoumarins in anticancer therapy, including multidrug-resistant (MDR) cancers, for which no ideal treatment is available at present [ 46 , 51 , 52 ]. For example, bergapten and xanthotoxin were found to have cytotoxicity against gastric (EPG85.257RDB), ovarian (A2780RCIS), and breast cancer (MCF7MX) cell lines overexpressing multidrug-resistant proteins (MDR1, MRP2, and BCRP) [ 53 ].…”

“…It was found that isopimpinellin affected PI3K activity and, consequently, neutrophil motility during the recruitment phase of inflammation. Additionally, some furanocoumarins have been reported to have chemotherapeutic synergistic potential when applied in combination with other anti-tumor drugs [ 45 , 46 ].…”

In vitro Cytotoxicity and Apoptosis-inducing Activity of Quercus infectoria Extracts in HeLa Cells

“…The finding indicated that cisplatin induced cell death through apoptosis and necrosis pathway. 36 Another significant feature of a potential anticancer agent is its ability to induce cell cycle arrest. The molecular mechanism underlying Pharmacognosy Journal, Vol 13, Issue 2, Mar-Apr, 2021 a compound's cytotoxicity can be understood from the cell cycle analysis 37 .…”

Enhancing Magnetic Hyperthermia Efficacy through Targeted Heat Shock Protein 90 Inhibition: Unveiling Immune-Mediated Therapeutic Synergy in Glioma Treatment

“…Further, HSP90 was also observed to be present in control cells owing to its natural expression in a variety of cancers. 35 In comparison to 2D cultures, higher expression of HSPs on HS exposure in 3D models suggests that cells in the 3D cultures are better able to mimic the actual TME. 36 Thereby, justifying the lesser cell death in 3D models as compared to 2D as reported in our study.…”