Cell death in wheat roots induced by the powdery mildew fungus Blumeria graminis f. sp. tritici

Deng, Xin; Li, Jiwei; Zhou, Zhuqing; Fan, Haiyan

doi:10.1007/s11104-009-0080-4

Cited by 15 publications

(8 citation statements)

References 37 publications

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“…DNA fragmentation, an indicator of PCD as assessed by TUNEL-positive nuclei, occurred in cortical cells of wheat and barley seminal roots and was used to phenotype RCS. (Henry and Deacon 1981;Liljeroth and Bryngelsson 2002;Deng et al 2010). In addition, the use of a single-cell pressure probe enabled the quantification of RCS along a root (Bingham 2007).…”

Section: Quantifying Rcsmentioning

confidence: 99%

Functional implications of root cortical senescence for soil resource capture

Schneider

Lynch

2017

Plant Soil

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Background Root phenes play a primary role in plant adaptation to edaphic stress. Understanding the functional implications of root phenotypes will enable the development of crop varieties with improved soil resource acquisition. Root cortical senescence (RCS) is a type of programmed cell death in cortical cells of several Poaceae species. Until recently there has been very little attention to the functional implications of RCS for water and nutrient capture. Scope We explore the functional implications of RCS as an adaptive trait for water and nutrient acquisition. The present review summarizes evidence from our own studies and other published work, and provides novel insights into the fitness landscape of RCS. Progress has recently been achieved in understanding the development and physiological implications of RCS. We propose that RCS is a useful trait for water and nutrient acquisition, particularly in edaphic stress conditions. Conclusions Further research is needed to understand the utility and tradeoffs of RCS in the context of specific environments, management practices, and phenotypic backgrounds. The utility of RCS for improved plant performance under edaphic stress merits investigation in the field. RCS may be a useful breeding target for improved soil resource capture in several major crop species including wheat, barley, and triticale.

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Section: Quantifying Rcsmentioning

confidence: 99%

Functional implications of root cortical senescence for soil resource capture

Schneider

Lynch

2017

Plant Soil

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“…The samples were first fixed in 3 % paraformaldehyde and embedded in the Tissue-Tek OCT 4583 compound (Sakura Finetek,USA), and then, sections (20 μm) were made on a Leica CM l850 (Germany). Detection methods followed Deng et al (2010): Frozen sections were stained with 5 μM H 2 DCF-DA for 1 h and then washed by 0.1 M phosphate buffer. Frozen sections were photographed under a fluorescence microscope (Nikon Eclipse 80i; Japan).…”

Section: Ros Fluorescence Detectionmentioning

confidence: 99%

Reactive oxygen species regulate programmed cell death progress of endosperm in winter wheat (Triticum aestivum L.) under waterlogging

Cheng

Zhang

et al. 2015

Protoplasma

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Previous studies have proved that waterlogging stress accelerates the programmed cell death (PCD) progress of wheat endosperm cells. A highly waterlogging-tolerant wheat cultivar Hua 8 and a waterlogging susceptible wheat cultivar Hua 9 were treated with different waterlogging durations, and then, dynamic changes of reactive oxygen species (ROS), gene expressions, and activities of antioxidant enzymes in endosperm cells were detected. The accumulation of ROS increased considerably after 7 days of waterlogging treatment (7 DWT) and 12 DWT in both cultivars compared with control group (under non-waterlogged conditions), culminated at 12 DAF (days after flowering) and reduced hereafter. Waterlogging resulted in a great increase of H2O2 and O2 (-) in plasma membranes, cell walls, mitochondrias, and intercellular spaces with ultracytochemical localization. Moreover, the deformation and rupture of cytomembranes as well as the swelling and distortion of mitochondria were obvious. Under waterlogging treatment conditions, catalase (CAT) gene expression increased in endosperm of Hua 8 but activity decreased. In addition, Mn superoxide dismutase (MnSOD) gene expression and superoxide dismutase (SOD) activity increased. Compared with Hua 8, both CAT, MnSOD gene expressions and CAT, SOD activities decreased in Hua 9. Moreover, ascorbic acid and mannitol relieve the intensifying of PCD processes in Hua 8 endosperm cells induced by waterlogging. These results indicate that ROS have important roles in the PCD of endosperm cells, the changes both CAT, MnSOD gene expressions and CAT, SOD activities directly affected the accumulation of ROS in two different wheat cultivars under waterlogging, ultimately led to the PCD acceleration of endosperm.

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“…The fluorescence intensity at 530 nm normalised to the protein content was used to express ROS presence. Fluorescence of DCF derived from ROS oxidation was also observed under a fluorescence binocular (Olympus SZX12; excitation 450-490 nm, emission C520 nm) (Deng et al 2010). …”

Section: Measurement Of Ros Levelmentioning

confidence: 99%

Biological responses of maize (Zea mays) plants exposed to chlorobenzenes. Case study of monochloro-, 1,4-dichloro- and 1,2,4-trichloro-benzenes

et al. 2011

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A 7-day-exposure time experiment was designed to investigate the phytotoxicity of chlorobenzenes (CBs) on Zea mays seedlings, focusing on the growth and generation of oxidative stress. Significant growth inhibition (based on biomass gain) was observed for exposure to monochlorobenzene (MCB), dichlorobenzene (DCB) and trichlorobenzene (TCB) concentrations higher than 10 mg l(-1). It would seem that CBs inhibit cell division, since the mitotic index decreased for roots exposed to DCB at 80 mg l(-1) dose (8%) and to all the TCB concentrations tested (20% inhibition). CBs exposure resulting in an increase in the oxidative stress response in maize seedlings [reactive oxygen species like H(2)O(2), antioxidant enzymes (POD, GR), lipid peroxidation] correlated to the compound's degree of chlorination, where damage increasing with the number of chlorine atoms (MCB < DCB < TCB). This biological response was also dependent on the dose-exposure. Z. mays exposed to CBs at concentrations <10 mg l(-1) did not induce sufficient oxidative damage to cause root cell death. Therefore, CBs at current environmental concentrations are unlikely to produce evident phytotoxic effects on Z. mays seedlings.

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Cell death in wheat roots induced by the powdery mildew fungus Blumeria graminis f. sp. tritici

Cited by 15 publications

References 37 publications

Functional implications of root cortical senescence for soil resource capture

Functional implications of root cortical senescence for soil resource capture

Reactive oxygen species regulate programmed cell death progress of endosperm in winter wheat (Triticum aestivum L.) under waterlogging

Biological responses of maize (Zea mays) plants exposed to chlorobenzenes. Case study of monochloro-, 1,4-dichloro- and 1,2,4-trichloro-benzenes

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