“…The primary screen was conducted through 10 HT-GPCA experiments, each measuring interactions of NS2 with $80 individual UPS proteins, 11 random, a priori non-interacting proteins (random reference set [RRS1]), and 5 known NS2 interacting partners (positive reference set [PRS]; Barouch-Bentov et al, 2016;Cai et al, 2016;Xiao et al, 2018;Tables S2 and S3). Luminescence values were measured, and data were analyzed by boxplot.…”