Cell death mechanisms in eukaryotes

Nirmala, J. Grace; Lopus, Manu

doi:10.1007/s10565-019-09496-2

Cited by 209 publications

(146 citation statements)

References 128 publications

Supporting

Mentioning

139

Contrasting

Unclassified

Order By: Relevance

“…Much more than DNA integrity is needed to accomplish embryo formation, and probably here lies the reason for impaired VOs development. Another possibility might be that VOs undergo necroptosis [i.e., another type of programmed cell death, independent from caspases and also known as inflammatory cell death ( 49 )] due to the exposure to Z-VAD-FMK and its inhibitory effect on caspase 8 ( 49 ). If this was the case, the absence of an increase in apoptotic markers would not be indicative of oocyte health, and the poor embryo development might be due to a non-apoptotic degeneration.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Apoptotic Pathways Improves DNA Integrity but Not Developmental Competence of Domestic Cat Immature Vitrified Oocytes

et al. 2020

View full text Add to dashboard Cite

Being a model for endangered wild felids, cryopreservation protocols for domestic cat oocytes are under continuous development. Immature vitrified oocytes (VOs) are a valuable resource for fertility preservation programs, but they often degenerate after warming and their in vitro development is poor. Since the exact mechanisms are not clear, this study assessed whether vitrification might trigger two apoptotic markers (DNA fragmentation and caspase activity, Experiment I) and the effects of a chemical inhibitor (i.e., the pan-caspase inhibitor Z-VAD-FMK) on the same markers (Experiment II) and on VOs in vitro development (Experiment III). The overarching aim was to check whether apoptosis inhibition might be a strategy to improve cat oocytes cryotolerance. In Experiment I, vitrification induced DNA fragmentation and increased caspase activity in VOs incubated for 24 h after warming (DNA fragmentation: 59.38%; caspase activity: 414.6 ± 326.8) compared to a fresh control (9.68%; 199.6 ± 178.3; p = 0.02). In Experiment II, the addition of Z-VAD-FMK to vitrification-warming and incubation media decreased DNA fragmentation and caspase activity (8.82%; 243.7 ± 106.9) compared to control (untreated) VOs (69.44%; 434.5 ± 248.3; p < 0.001). In Experiment III, Z-VAD-FMK brought maturation rates of treated VOs close to those of fresh oocytes (53.13 and 65.38%, respectively, p = 0.057), but there were no differences in VOs embryo development (cleavage rates; Z-VAD-FMK-treated VOs: 34.38%; control VOs: 31.78%; p = 0.69). In summary, vitrification increased apoptotic markers in cat VOs, and while Z-VAD-FMK was able to hinder DNA damage and caspase activity, its addition was not determinant for embryo development. To make the best use of VOs, other oocyte in vitro maturation and embryo culture strategies, such as the addition of other inhibitors or their prolonged use, should be investigated.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibition of Apoptotic Pathways Improves DNA Integrity but Not Developmental Competence of Domestic Cat Immature Vitrified Oocytes

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Neuronal apoptosis also significantly contributes to secondary injury [36,37]. In addition to apoptosis, necroptosis, a recently identified programmed cell death bearing resemblance to both apoptosis and necrosis, has also been demonstrated to play an indispensable role in secondary neuronal cell death and neuroinflammation post-TBI [38,39]. Mechanically, upon pathogenic stimuli following TBI, TNF-α-induced receptor-interacting protein 1 activation contributes to the formation of the so-called necrosome, a complex necessary for necroptosis [40,41].…”

Section: Introductionmentioning

confidence: 99%

Dual roles of astrocytes in plasticity and reconstruction after traumatic brain injury

et al. 2020

View full text Add to dashboard Cite

Traumatic brain injury (TBI) is one of the leading causes of fatality and disability worldwide. Despite its high prevalence, effective treatment strategies for TBI are limited. Traumatic brain injury induces structural and functional alterations of astrocytes, the most abundant cell type in the brain. As a way of coping with the trauma, astrocytes respond in diverse mechanisms that result in reactive astrogliosis. Astrocytes are involved in the physiopathologic mechanisms of TBI in an extensive and sophisticated manner. Notably, astrocytes have dual roles in TBI, and some astrocyte-derived factors have double and opposite properties. Thus, the suppression or promotion of reactive astrogliosis does not have a substantial curative effect. In contrast, selective stimulation of the beneficial astrocytederived molecules and simultaneous attenuation of the deleterious factors based on the spatiotemporalenvironment can provide a promising astrocyte-targeting therapeutic strategy. In the current review, we describe for the first time the specific dual roles of astrocytes in neuronal plasticity and reconstruction, including neurogenesis, synaptogenesis, angiogenesis, repair of the blood-brain barrier, and glial scar formation after TBI. We have also classified astrocyte-derived factors depending on their neuroprotective and neurotoxic roles to design more appropriate targeted therapies.

show abstract

“…Nevertheless, it is necessary to mediate the probably correct apoptosis as well as programmed cell death genetically. In the cancer cell line, apoptosis might occur through a diver's molecular mechanisms [44,45].…”

Section: Schisandrin B and Cancermentioning

confidence: 99%

A Comprehensive Review on Schisandrin B and Its Biological Properties

Nasser

Zhu

Chen

et al. 2020

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Nature is a vast source of bioactive molecules and has provided an active and efficient reservoir for drug discovery. Among natural compounds, one of the most promising is Schisandrin B (Sch B), isolated from Schisandra chinensis, which was documented to possess diversified pharmacokinetic propriety, among them antioxidant, anti-inflammation, cardioprotection, and neuroprotection. Due to its large biological properties, Sch B was recorded to be a potent cure for several diseases by targeting several signaling pathways. This review is aimed at emphasizing the recent data on the biological properties of Sch B among the molecular mechanism of this drug on tumoral, cardiac, and neural diseases. The data suggest that the antitumor activities of Sch B were mainly through apoptosis and cell cycle arrest at the diver’s stage. It is reported that Sch B could be used as effective chemotherapy, neuroprotection, and cardioprotection since it possesses a spectrum of biological activities; however, further investigations on the mechanism of its action and preclinical trials are still mandatory to further validate the potential of this natural drug candidate.

show abstract

Cell death mechanisms in eukaryotes

Cited by 209 publications

References 128 publications

Inhibition of Apoptotic Pathways Improves DNA Integrity but Not Developmental Competence of Domestic Cat Immature Vitrified Oocytes

Inhibition of Apoptotic Pathways Improves DNA Integrity but Not Developmental Competence of Domestic Cat Immature Vitrified Oocytes

Dual roles of astrocytes in plasticity and reconstruction after traumatic brain injury

A Comprehensive Review on Schisandrin B and Its Biological Properties

Contact Info

Product

Resources

About