Cell-derived matrices (CDM)—Methods, challenges and applications

Cheng, Haobo; Yuan, Minting; Li, C W; Chan, Barbara Pui

doi:10.1016/bs.mcb.2020.01.001

Cited by 11 publications

(9 citation statements)

References 68 publications

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“…The use of CDMs to study fundamental principles of cell-matrix interactions is an attractive approach that has increasingly grown in popularity in recent years 8,10,41,68 In addition to structural differences, CDMs had protein compositions specific to their developmental stage. Fetal CDMs were enriched for the three primary chains of Col 6, the loss of which is associated with impaired skeletal muscle function and regeneration due to metabolic deficits 62,63 .…”

Section: Discussionmentioning

confidence: 99%

“…Cell-derived matrices (CDMs) have emerged as a tractable experimental platform to model the ECM in vitro. CDMs are comprised of the isolated matrix produced by cultured cells -typically stromal cells from the tissue of interest 7,8 , which produce a large proportion of interstitial ECM 9 .…”

Section: Introductionmentioning

confidence: 99%

“…However, current methods for CDM matrix production and decellularization present caveats that limit CDM fidelity to mimic native ECM properties, with unknown influences on subsequent cell biology studies. Many protocols aim to improve the durability of ECM by promoting adhesion with pre-adsorbed and/or crosslinked matrix components, such as gelatin (denatured collagen) or fibronectin (FN) 7,10,11 , and increasing matrix assembly and thickness by promoting collagen I synthesis and secretion 8,10 . These types of approaches perturb the endogenous ECM composition by artificially increasing individual matrix proteins, sometimes to supraphysiological levels.…”

Section: Introductionmentioning

confidence: 99%

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Cardiac Cell-Derived Matrices Impart Age-Specific Functional Properties to Human Cardiomyocytes

Rockwood²,

Silva³

et al. 2020

Preprint

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Cell-derived matrices (CDMs) isolated from cultured cells provide complex and tissue-specific biochemical and physical cues derived from the extracellular matrix (ECM) that are lacking in typical tissue culture environments. However, current methods enhance ECM adhesion and thickness via introduction and promotion of singular matrix proteins, skewing the matrix composition, and confounding comparisons between CDMs. Here we developed a protocol that enhances CDM stability and deposition, respectively, by combining an L-polydopamine surface coating with Ficoll macromolecular crowing prior to hypotonic decellularization. This methodology was applied to the study of age-dependent phenotypic and functional changes observed in cardiac ECM by comparing the morphologic, electrophysiological and metabolic response of cardiomyocytes in response to CDMs produced by fetal and adult cardiac fibroblasts. Furthermore, mass spectrometry proteomics identified the enrichment of collagen VI in fetal CDMs, which we determined via siRNA-mediated silencing during CDM production to be necessary for maximal oxidative respiration in cardiomyocytes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Cardiac Cell-Derived Matrices Impart Age-Specific Functional Properties to Human Cardiomyocytes

Rockwood²,

Silva³

et al. 2020

Preprint

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“…78 Moreover, culturing chondrocytes in 2D cultures is challenging. 87 Chondrocyte cultured in vitro in monolayers can easily dedifferentiate to a more fibroblastic phenotype after long expansion time and multiple passaging. 88 The dedifferentiation is characterized by higher expression of COL1 genes and decreased expression and production of cartilage-specific proteins such as type-II collagen (COL2), GAGs, and aggrecan (ACAN).…”

Section: Integration Of 3d Col1 Based Scaffolds With Cellsmentioning

confidence: 99%

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

et al. 2021

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The bones can be viewed as both an organ and a material. As an organ, the bones give structure to the body, facilitate skeletal movement, and provide protection to internal organs. As a material, the bones consist of a hybrid organic/inorganic threedimensional (3D) matrix, composed mainly of collagen, noncollagenous proteins, and a calcium phosphate mineral phase, which is formed and regulated by the orchestrated action of a complex array of cells including chondrocytes, osteoblasts, osteocytes, and osteoclasts. The interactions between cells, proteins, and minerals are essential for the bone functions under physiological loading conditions, trauma, and fractures. The organization of the bone's organic and inorganic phases stands out for its mechanical and biological properties and has inspired materials research. The objective of this review is to fill the gaps between the physical and biological characteristics that must be achieved to fabricate scaffolds for bone tissue engineering with enhanced performance. We describe the organization of bone tissue highlighting the characteristics that have inspired the development of 3D cell-laden collagenous scaffolds aimed at replicating the mechanical and biological properties of bone after implantation. The role of noncollagenous macromolecules in the organization of the collagenous matrix and mineralization ability of entrapped cells has also been reviewed. Understanding the modulation of cell activity by the extracellular matrix will ultimately help to improve the biological performance of 3D cell-laden collagenous scaffolds used for bone regeneration and repair as well as for in vitro studies aimed at unravelling physiological and pathological processes occurring in the bone.

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“…They have been used for numerous tissue engineering applications including repair of musculotendinous structures, lower urinary tract reconstruction, esophageal replacement, skin healing (chronic wound), and upper airway reconstruction [Niklason, 2018;Sarrafian et al, 2018]. However, due to the complex architecture of the ECM, designing and engineering a mimetic of this complex structure poses a greater challenge in tissue engineering [Hussey et al, 2018;Cheng et al, 2020].…”

Section: Introductionmentioning

confidence: 99%

Effect of Cell-Derived Matrices on Growth and Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells

Selvaraj

Rupert

Nandabalan

et al. 2022

Cells Tissues Organs

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Cell derived matrices (CDMs) are scaffolds constructed by decellularization of cellular matrices from different tissues and organs. Since cell derived matrices mimic the ECM of native tissues, CDM plays an essential role in the preparation of bioscaffolds. CDM scaffolds from Mesenchymal Stem Cells (MSCs) have been reported to support cell adhesion and proliferation of its own cells. Therefore, in this study we aimed to test if growth of human Wharton’s jelly derived MSCs (hWJ-MSCs) may be enhanced when cultured on their own cell derived matrices. To do this, MSCs were induced to generate ECM using ascorbic acid. Thus, obtained matrices were decellularized and characterized quantitatively for changes in their biochemical components (total protein, collagen, glycosaminoglycans) and qualitatively for fibronectin, laminin and collagen (I & IV) by immunostaining. Our results show the retention of essential ECM components in the decellularized WJ-CDM. The influence of WJ-MSC-derived CDM on proliferation and differentiation of WJ-MSCs were evaluated by comparing their growth on collagen and fibronectin only coated plates. A non-coated tissue culture polystyrene plate (TCPS/WC) served as control. Our cell proliferation results show that no significant changes were observed in the proliferation of MSCs when cultured on WJ-MSC derived CDM as compared to the bio-coated and non-coated cultures. However, gene expression analysis of the differentiation process showed that osteogenic and adipogenic differentiation potential of the WJ-MSCs was significantly increased upon culturing them on WJ-MSC-CDM. In conclusion, the present study reveals that the WJ-MSCs cultured on WJ-MSC-CDM may augment osteogenic and adipogenic differentiation.

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Cell-derived matrices (CDM)—Methods, challenges and applications

Cited by 11 publications

References 68 publications

Cardiac Cell-Derived Matrices Impart Age-Specific Functional Properties to Human Cardiomyocytes

Cardiac Cell-Derived Matrices Impart Age-Specific Functional Properties to Human Cardiomyocytes

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

Effect of Cell-Derived Matrices on Growth and Differentiation of Human Wharton’s Jelly-Derived Mesenchymal Stem Cells

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