Cell disassembly during apoptosis

Smith, A. N.; Parkes, Michael A. F.; Atkin-Smith, Georgia K.; Tixeira, Rochelle; Poon, Ivan K. H.

doi:10.15347/wjm/2017.008

Cited by 4 publications

(2 citation statements)

References 18 publications

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“…Yellow circle around nucleus : 4N DNA content; double-/single-headed arrows between cells : reversible/ irreversible phenotypic transitions in response to changing environments; green arrow : change in growth factor levels; red : change in Trail exposure. Image credits : apoptotic cell [78]; quiescent cell: https://en.wikipedia.org/wiki/Cell_culture#/media/File:HeLa_cells_stained_with_Hoechst_33258.jpg ; mitotic spindle: https://en.wikipedia.org/wiki/Cell_division#/media/File:Kinetochore.jpg .…”

Section: Resultsmentioning

confidence: 99%

Boolean model of growth signaling, cell cycle and apoptosis predicts the molecular mechanism of aberrant cell cycle progression driven by hyperactive PI3K

et al. 2019

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The PI3K / AKT signaling pathway plays a role in most cellular functions linked to cancer progression, including cell growth, proliferation, cell survival, tissue invasion and angiogenesis. It is generally recognized that hyperactive PI3K / AKT1 are oncogenic due to their boost to cell survival, cell cycle entry and growth-promoting metabolism. That said, the dynamics of PI3K and AKT1 during cell cycle progression are highly nonlinear. In addition to negative feedback that curtails their activity, protein expression of PI3K subunits has been shown to oscillate in dividing cells. The low- PI3K /low- AKT1 phase of these oscillations is required for cytokinesis, indicating that oncogenic PI3K may directly contribute to genome duplication. To explore this, we construct a Boolean model of growth factor signaling that can reproduce PI3K oscillations and link them to cell cycle progression and apoptosis. The resulting modular model reproduces hyperactive PI3K -driven cytokinesis failure and genome duplication and predicts the molecular drivers responsible for these failures by linking hyperactive PI3K to mis-regulation of Polo-like kinase 1 ( Plk1 ) expression late in G2. To do this, our model captures the role of Plk1 in cell cycle progression and accurately reproduces multiple effects of its loss: G2 arrest, mitotic catastrophe, chromosome mis-segregation / aneuploidy due to premature anaphase, and cytokinesis failure leading to genome duplication, depending on the timing of Plk1 inhibition along the cell cycle. Finally, we offer testable predictions on the molecular drivers of PI3K oscillations, the timing of these oscillations with respect to division, and the role of altered Plk1 and FoxO activity in genome-level defects caused by hyperactive PI3K . Our model is an important starting point for the predictive modeling of cell fate decisions that include AKT1 -driven senescence, as well as the non-intuitive effects of drugs that interfere with mitosis.

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Section: Resultsmentioning

confidence: 99%

Boolean model of growth signaling, cell cycle and apoptosis predicts the molecular mechanism of aberrant cell cycle progression driven by hyperactive PI3K

et al. 2019

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“…Apoptotic cells can be distinguished by their unique morphological changes from other cells. These signatures of apoptotic cells in appearance include shrinkage of cells, chromatin condensation, plasma membrane blebbing, and formation of apoptotic bodies, which was previously described by Smith et al (2017).…”

Section: Morphology Changes In Cells Exposed To Simpor Leaf Extract (...mentioning

confidence: 60%

Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Rahayu¹,

Chang²,

Supiyani³

et al. 2023

J App Pharm Sc

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Cancer is a malignant tumor with characteristics of uncontrolled cell growth, poor metastasis, leading to the dysfunction of organs and death of patients. Many efforts have been made, including the exploration of natural products. The purpose of this study was to determine the cytotoxicity of the Dillenia suffruticosa leaf extract against the cell lines MCF-7 and HepG2. The method used is an experimental research design with a completely randomized design. The treatment group consisted of the leaf extract of 500, 1,000, 2,000, 5,000, and 9,000 ppm for the brine shrimp lethality test (BSLT). The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) test used 10, 15, 20, 25, and 50 ppm concentrations and control (without extract). The data on the BSLT test was determined by analyzing the lethal concentration 50 (LC 50 ) value using the probit analysis, while the MTT test was determined by analyzing the IC 50 and analysis of variance test. The BSLT test results showed an LC 50 value of 5,221 ppm, which means that the leaf extract is not toxic. Simpor leaf extract at 25 ppm with IC50 of 88.52 was seen as the best concentration for liver cancer treatment. It can be concluded that D. suffruticosa shows cytotoxicity against the cell lines MCF-7 and HepG2 and can be used as a candidate for breast and liver cancer treatment.

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Recent advances in nanotechnology-based cell toxicity evaluation approaches relevant to biofuels and bioenergy applications

Nagappan¹,

Jeno²,

Viveka³

et al. 2021

Nanomaterials

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Cell disassembly during apoptosis

Cited by 4 publications

References 18 publications

Boolean model of growth signaling, cell cycle and apoptosis predicts the molecular mechanism of aberrant cell cycle progression driven by hyperactive PI3K

Boolean model of growth signaling, cell cycle and apoptosis predicts the molecular mechanism of aberrant cell cycle progression driven by hyperactive PI3K

Simpor leaf extract- (Dillenia suffruticosa Martelli) induced apoptosis of the MCF-7 and HepG2 cell lines

Recent advances in nanotechnology-based cell toxicity evaluation approaches relevant to biofuels and bioenergy applications

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