Cell Enrichment and Immunochemical Staining

An efficient method to limit microglia-dependent effects in astroglial cultures

“…Newer techniques are based on physical criteria like size, shape, and density. They include filtration and centrifugation approaches (Fuss et al, 2009;Lara and Chalmers, 2005;Graham, 2001), and also affinity approaches based upon biochemical cell surface and biophysical characteristics (Cho et al, 2010). Other approaches are the use of anti-mitotic agents to inhibit microglial growth in primary astrocyte cultures (Hamby et al, 2006a;Solenov et al, 2004) or the differential adherence of various glial cells on cell culture plates (Jana et al, 2007).…”