Cell free DNA; diagnostic and prognostic approaches to oncology

Arshad, None Sjawal; Khawar, Muhammad Babar; Hassan, Ali; Ali, Amir R.; Sohail, Abdullah Muhammad; Mukhtar, Maryam; Abbasi, Muddasir Hassan; Shiekh, Nadeem; Azam, None Arwa; Shahzaman, Sara; Hamid, Syeda Eisha

doi:10.1016/j.adcanc.2022.100052

Cited by 5 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The solution was then further purified via dialysis against distilled water. CaCO 3 -PEI [18]. CaCO 3 -PEI NPs were prepared via chemical precipitation.…”

Section: Synthesis Of Scavengersmentioning

confidence: 99%

“…DAMPs and PAMPs have been clinically found to be increased in trauma [ 7 , 8 , 9 ], inflammatory disease [ 10 , 11 , 12 , 13 ], and cancer [ 14 , 15 , 16 ]. Cell-free nucleic acids are being used for early disease detection, and high levels of DAMPs and PAMPs have been correlated to lower prognoses in many disease states [ 7 , 12 , 13 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 ]. NABNs can reduce TLR overactivation by acting as molecular scavengers that bind nucleic acids and other charged molecules, including DAMPs and PAMPs [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Comparative Analysis of Nucleic Acid-Binding Polymers as Potential Anti-Inflammatory Nanocarriers

Bhansali,

Akinade,

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

Conventionally, nanocarriers are used to regulate the controlled release of therapeutic payloads. Increasingly, they can also be designed to have an intrinsic therapeutic effect. For example, a positively charged nanocarrier can bind damage-associated molecular patterns, inhibiting toll-like receptor (TLR) pathway activation and thus modulating inflammation. These nucleic acid-binding nanomaterials (NABNs), which scavenge pro-inflammatory stimuli, exist in diverse forms, ranging from soluble polymers to nanoparticles and 2D nanosheets. Unlike conventional drugs that primarily address inflammation symptoms, these NABPs target the upstream inflammation initiation pathway by removing the agonists responsible for inflammation. Many NABNs have demonstrated effectiveness in murine models of inflammatory diseases. However, these scavengers have not been systematically studied and compared within a single setting. Herein, we screen a subset of the most potent NABNs to define their relative efficiency in scavenging cell-free nucleic acids and inhibiting various TLR pathways. This study helps interpret existing in vivo results and provides insights into the future design of anti-inflammatory nanocarriers.

show abstract

“…The solution was then further purified via dialysis against distilled water. CaCO 3 -PEI [18]. CaCO 3 -PEI NPs were prepared via chemical precipitation.…”

Section: Synthesis Of Scavengersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Nucleic Acid-Binding Polymers as Potential Anti-Inflammatory Nanocarriers

Bhansali,

Akinade,

et al. 2023

Pharmaceutics

View full text Add to dashboard Cite

show abstract

“…Directed distribution of exRNA-loaded EVs for gene therapy and treatments targeting exRNAs have been implicated in kidney disease development, which is a potential therapeutic strategy [ 106 ]. Recently, a study investigated the effectiveness of cell-free DNA (cfDNA) [ 108 ] and exRNAs in cancer cases by identifying viable biomarkers [ 109 ].…”

Section: Exrnas As Therapeuticsmentioning

confidence: 99%

Multifaceted roles of extracellular RNAs in different diseases

Sohail

Khawar²,

Ali

et al. 2022

Military Med Res

Self Cite

View full text Add to dashboard Cite

Extracellular RNAs (exRNAs) are novel circulating factors that can be used as biomarkers in various diseases. Their unique and diverse kinds, as well as their role as biomarkers, make them significant biomarkers. There has been immense work carried out since the discovery of exRNAs in circulation and other biological fluids to catalog and determine whether exRNAs may be utilized as indicators for health and illness. In this review, we aim to understand the current state of exRNAs in relation to various diseases and their potential as biomarkers. We will also review current issues and challenges faced in using exRNAs, with clinical and lab trials, that can be used as viable markers for different diseases.

show abstract