2024
DOI: 10.1101/2024.02.12.580006
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Cell-free expression and biochemical characterization of polysaccharide-synthesizing glycosyltransferases

Dharanidaran Jayachandran,
Amar D. Parvate,
Jory T. Brookreson
et al.

Abstract: Polysaccharides are a major class of natural polymers found abundantly across all major life forms and play a critical role as structural, metabolic, or functional components in biomolecular processes. Some polysaccharides like cellulose and hyaluronan are synthesized by membrane-bound family-2 glycosyltransferases (GTs). Despite the fact that the GT-2 family has the maximum number of deposited sequences, the biochemistry of GT-2 family enzymes is still poorly understood due to difficulties associated with GT … Show more

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